Abstract:
:Loss of p53's tumor-suppressive function, either via TP53 mutation or hyperactive p53 inhibitory proteins, is one of the most frequent events in the development of human cancer. Here, we describe a strategy of pharmacologically inhibiting iASPP, a negative regulator of p53, to restore wild-type p53's tumor-suppressive function. iASPP knockdown in the colon cancer cell line HCT116 efficiently promoted p53's transcriptional activity and induced p53-dependent cell death, suggesting a key role for iASPP in silencing p53 in this cell line. Screening of a preclinical and clinical drug library using isogenic HCT116 cell models revealed that cyclin-dependent kinase 9 (CDK9) inhibitors preferentially inhibit p53+/+, rather than p53-/-, cells. Mechanistically, CDK9 inhibitors downregulated iASPP at the transcriptional level. This downregulation was dose- and time-dependent. CDK9 inhibitors further showed synergistic effects in killing p53+/+ HCT116 cells when combined with the MDM2 inhibitor Nutlin-3. In a large TCGA pan-cancer cohort, iASPP overexpression predicted poor overall survival (OS) in wild-type p53 patients, with worse OS observed when MDM2 was simultaneously overexpressed. Our study identifies CDK9 inhibitors as p53-reactivating agents, and proposes a strategy to treat cancer by efficiently reactivating p53 via the concurrent inhibition of iASPP and MDM2.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Wu J,Liang Y,Tan Y,Tang Y,Song H,Wang Z,Li Y,Lu Mdoi
10.1016/j.cellsig.2019.109508subject
Has Abstractpub_date
2020-03-01 00:00:00pages
109508eissn
0898-6568issn
1873-3913pii
S0898-6568(19)30304-3journal_volume
67pub_type
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journal_title:Cellular signalling
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pub_type: 杂志文章,评审
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journal_title:Cellular signalling
pub_type: 杂志文章
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