Erythropoietin receptor-mediated Egr-1 activation: structural requirements and functional implications.

Abstract:

:The transcription factor Egr-1 is encoded by an immediate early response gene and has been shown to be a key regulator in the induction of apoptosis, mitogenesis and differentiation. It is rapidly induced by different stimuli including the glycoprotein hormone erythropoietin. In this report, we analyse the role of different erythropoietin receptor substructures for the activation of Egr-1 and the functional consequences of Egr-1 overexpression in the erythroleukemic cell line ELM-I-1. The investigation of receptor variants revealed that the activity of JAK2 and the phosphorylation of receptor tyrosine residues are essential preconditions for the ability to target Egr-1. Furthermore, we observed a close correlation of the abilities of receptors to activate the Ras-MAPK pathway and Egr-1. Using mass spectrometry we identified the Ras-GTPase-activating protein-SH3-domain-binding protein 1 (G3BP-1), a component of the Ras network of proteins, as an Egr-1 interacting protein in EPO stimulated ELM-I-1 cells. The overexpression of Egr-1 in these cells resulted in an enhanced rate of spontaneous erythroid differentiation.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Schulze C,Büchse T,Mikkat S,Bittorf T

doi

10.1016/j.cellsig.2008.06.013

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

1848-54

issue

10

eissn

0898-6568

issn

1873-3913

pii

S0898-6568(08)00187-3

journal_volume

20

pub_type

杂志文章
  • Effects of retinoic acid on signalling by prostaglandin E2 in osteoblast-like cells.

    abstract::We investigated the effects of retinoic acid (RA) on the signalling pathways by prostaglandin E2 (PGE2) in osteoblast-like MC3T3-E1 cells. The pretreatment with RA significantly inhibited the formation of inositol phosphates induced by 10 microM PGE2 in a dose-dependent manner in the range between 0.1 nM and 0.1 micro...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(93)90080-6

    authors: Kotoyori J,Tokuda H,Oiso Y,Kozawa O

    更新日期:1993-07-01 00:00:00

  • Hsp90 functions to balance the phosphorylation state of Akt during C2C12 myoblast differentiation.

    abstract::The function of the 90-kDa heat shock protein (Hsp90) is essential for the regulation of a myriad of signal transduction cascades that control all facets of a cell's physiology. Akt (PKB) is an Hsp90-dependent serine-threonine kinase that plays critical roles in the regulation of muscle cell physiology, including role...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2005.03.006

    authors: Yun BG,Matts RL

    更新日期:2005-12-01 00:00:00

  • Mechanisms of action of antidepressants: from neurotransmitter systems to signaling pathways.

    abstract::Antidepressants are commonly used in the treatment of anxiety and depression, medical conditions that affect approximately 17-20% of the population. The clinical effects of antidepressants take several weeks to manifest, suggesting that these drugs induce adaptive changes in brain structures affected by anxiety and de...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2004.12.007

    authors: Taylor C,Fricker AD,Devi LA,Gomes I

    更新日期:2005-05-01 00:00:00

  • DDX17 (P72), a Sox2 binding partner, promotes stem-like features conferred by Sox2 in a small cell population in estrogen receptor-positive breast cancer.

    abstract::We have previously demonstrated the existence of two phenotypically distinct cell subsets in estrogen receptor (ER)-positive breast cancer (BC) based on their differential response to a Sox2 reporter (SRR2), with reporter responsive (RR) cells being more tumorigenic and stem-like than reporter unresponsive (RU) cells....

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2015.11.004

    authors: Alqahtani H,Gopal K,Gupta N,Jung K,Alshareef A,Ye X,Wu F,Li L,Lai R

    更新日期:2016-02-01 00:00:00

  • Loss of Raf-1-binding activity of v-Ha-Ras by the deletion of amino acid residues 64-72 and 143-151.

    abstract::In order to elucidate the molecular events in signal transduction, examination of the interaction between Ras and Raf-1 seems crucial. Many Raf-1 mutants have been investigated in terms of their binding activities to Ras, where only a few Ras mutants have been examined thus far. We have investigated the Raf-1-binding ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(96)00078-2

    authors: Hiwasa T,Kasama M,Nakadai T,Sawada T,Sakiyama S

    更新日期:1996-08-01 00:00:00

  • Tamoxifen blocks both proliferation and voltage-dependent K+ channels of neuroblastoma cells.

    abstract::The effects of tamoxifen (TAM) on cell proliferation and voltage-dependent K+ channels were studied on the mouse neuroblastoma cells NG 108-15. TAM inhibited cell proliferation with an effective dose inducing a half maximum effect (ED50) of 2 microM and was cytotoxic from and beyond 2.5 microM. TAM accelerated the app...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(90)90069-m

    authors: Rouzaire-Dubois B,Dubois JM

    更新日期:1990-01-01 00:00:00

  • Homer1 knockdown protects dopamine neurons through regulating calcium homeostasis in an in vitro model of Parkinson's disease.

    abstract::Homer1 protein is an important scaffold protein at postsynaptic density and has been demonstrated to play a central role in calcium signaling in the central nervous system. The aim of this study was to investigate the effects of Homer1 knockdown on MPP(+) induced neuronal injury in cultured dopamine (DA) neurons. We f...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.09.004

    authors: Chen T,Yang YF,Luo P,Liu W,Dai SH,Zheng XR,Fei Z,Jiang XF

    更新日期:2013-12-01 00:00:00

  • CIKS (Act1 or TRAF3IP2) mediates high glucose-induced endothelial dysfunction.

    abstract::Hyperglycemia-induced endothelial dysfunction is characterized by enhanced inflammatory cytokine and adhesion molecule expression, and endothelial-monocyte adhesion. The adapter molecule CIKS (connection to IKK and SAPK/JNK; also known as Act1 or TRAF3IP2) is an upstream regulator of NF-κB and AP-1, and plays a role i...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2012.10.009

    authors: Venkatesan B,Valente AJ,Das NA,Carpenter AJ,Yoshida T,Delafontaine JL,Siebenlist U,Chandrasekar B

    更新日期:2013-01-01 00:00:00

  • Difference between follistatin isoforms in the inhibition of activin signalling: activin neutralizing activity of follistatin isoforms is dependent on their affinity for activin.

    abstract::We demonstrate the difference between the follistatin isoforms (FS-288 and FS-315), two activin-binding proteins, in the neutralizing activity for activin signalling. Transcriptional reporter assay using 3TP-Lux, an activin-responsive reporter construct, showed that the inhibitory effect of FS-288 on activin-induced t...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(00)00099-1

    authors: Hashimoto O,Kawasaki N,Tsuchida K,Shimasaki S,Hayakawa T,Sugino H

    更新日期:2000-08-01 00:00:00

  • RanBPM interacts with TβRI, TRAF6 and curbs TGF induced nuclear accumulation of TβRI.

    abstract::Transforming growth factor β (TGF-β), a cytokine, and its receptors play a vital role during normal embryogenesis, cell proliferation, differentiation, apoptosis and migration. Ran-binding protein in the microtubule-organizing center (RanBPM) serves as a scaffold protein that has been shown to interact with many other...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2013.09.019

    authors: Zhang J,Ma W,Tian S,Fan Z,Ma X,Yang X,Zhao Q,Tan K,Chen H,Chen D,Huang BR

    更新日期:2014-01-01 00:00:00

  • IL-2 activation of a PI3K-dependent STAT3 serine phosphorylation pathway in primary human T cells.

    abstract::Interleukin-2 (IL-2) is the major growth factor of activated T lymphocytes. By inducing cell cycle progression and protection from apoptosis in these cells, IL-2 is involved in the successful execution of an immune response. Upon binding its receptor, IL-2 activates a variety of signal transduction pathways, including...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/s0898-6568(03)00003-2

    authors: Fung MM,Rohwer F,McGuire KL

    更新日期:2003-06-01 00:00:00

  • Cellular signaling in pseudoxanthoma elasticum: an update.

    abstract::Pseudoxanthoma elasticum is an autosomal recessive genodermatosis with variable expression, due to mutations in the ABCC6 or ENPP1 gene. It is characterized by elastic fiber mineralization and fragmentation, resulting in skin, eye and cardiovascular symptoms. Significant advances have been made in the last 20 years wi...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2018.12.009

    authors: Van Gils M,Nollet L,Verly E,Deianova N,Vanakker OM

    更新日期:2019-03-01 00:00:00

  • Homeostatic interactions between MEKK3 and TAK1 involved in NF-kappaB signaling.

    abstract::Several members of the mitogen-activated protein kinase kinase kinase (MAP3K) family including MEKK3 and TGFbeta-activating kinase (TAK1) play nonredundant roles in activation of the NF-kappaB transcription factor. However, the mechanism by which MEKK3 mediates NF-kappaB signaling is not fully understood. In this repo...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2007.12.007

    authors: Di Y,Li S,Wang L,Zhang Y,Dorf ME

    更新日期:2008-04-01 00:00:00

  • Regulation of C1-Ten protein tyrosine phosphatase by p62/SQSTM1-mediated sequestration and degradation.

    abstract::C1-Ten is a member of the tensin family of focal adhesion molecules but recent studies suggest it plays a more active role in many biological processes because of its potential association with diabetes and cancers. However, relatively little is known about the regulation of C1-Ten, such as changes in its protein leve...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.07.033

    authors: Koh A,Park D,Jeong H,Lee J,Lee MN,Suh PG,Ryu SH

    更新日期:2014-11-01 00:00:00

  • Leptin upregulates VEGF in breast cancer via canonic and non-canonical signalling pathways and NFkappaB/HIF-1alpha activation.

    abstract::High levels of VEGF and leptin are strongly linked to worse prognosis of breast cancer. Leptin signalling upregulates VEGF in human and mouse mammary tumor cells (MT), but the specific molecular mechanisms are largely unknown. Pharmacologic and genetic approaches were used to dissect the mechanism of leptin regulation...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2010.05.003

    authors: Gonzalez-Perez RR,Xu Y,Guo S,Watters A,Zhou W,Leibovich SJ

    更新日期:2010-09-01 00:00:00

  • HSP27 modulates survival signaling networks in cells treated with curcumin and TRAIL.

    abstract::The combination of curcumin and TRAIL and their role in enhancing apoptotic cell death has been reported by many studies. However, the exact molecular mechanism of apoptosis mediated by curcumin and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is not yet completely understood. In this study, we obse...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2012.03.009

    authors: Kim J,Kim SY,Kang S,Yoon HR,Sun BK,Kang D,Kim JH,Song JJ

    更新日期:2012-07-01 00:00:00

  • Gax inhibits perivascular preadipocyte biofunction mediated by IGF-1 induced FAK/Pyk2 and ERK2 cooperative pathways.

    abstract::Perivascular adipocyte (PVAC) biofunctions were closely related to cardiovascular diseases; its specific biological mechanisms remained unclear. How to adjust PVAC functions of vascular cells is an important topic. The present study was designed to investigate whether FAK/Pyk2 and ERK1/2 MAPK signaling pathways partic...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.09.017

    authors: Liu P,Feng J,Kong F,Lu Q,Xu H,Meng J,Jiang Y

    更新日期:2014-12-01 00:00:00

  • Adenosine inhibits divalent cation influx across human neutrophil plasma membrane via surface adenosine A2 receptors.

    abstract::Adenosine and its analogues inhibited increases in divalent cation influx stimulated by platelet-activating factor (PAF) and formyl-methionyl-leucyl-phenylalanine (FMLP) in a dose-dependent fashion. This effect was antagonized by theophylline, an adenosine receptor antagonist. When extracellular adenosine was removed ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(92)90023-2

    authors: Tsuruta S,Ito S,Mikawa H

    更新日期:1992-09-01 00:00:00

  • TGFbeta-induced GRK2 expression attenuates AngII-regulated vascular smooth muscle cell proliferation and migration.

    abstract::Through diametric actions, the transforming growth factor beta (TGFbeta) and Angiotensin II (AngII) play important roles in regulating various biological responses such as cell proliferation and migration. Signaling initiated by TGFbeta and AngII occurs through two structurally and functionally distinct receptor super...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2009.01.037

    authors: Guo J,Chen H,Ho J,Mancini J,Sontag T,Laporte SA,Richard DE,Lebrun JJ

    更新日期:2009-06-01 00:00:00

  • Effect of H-ras proteins on the activity of polyphosphoinositide phospholipase C in HL60 membranes.

    abstract::The present study was undertaken to investigate whether purified ras proteins can affect the activity of polyphosphoinositide specific phospholipase C in a cell-free membrane system. For this purpose we used homogenous preparations of the proto-oncogenic (H-ras(gly 12)) and the oncogenic (H-ras(val 12)) forms of the h...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/0898-6568(90)90050-k

    authors: Cockcroft S,Bar-Sagi D

    更新日期:1990-01-01 00:00:00

  • Expression and significance of HERG (KCNH2) potassium channels in the regulation of MDA-MB-435S melanoma cell proliferation and migration.

    abstract::The human ether-a-go-go related gene (HERG) potassium channel has elicited intense scientific interest due to its role in cardiac repolarization and its association with arrhythmia and sudden cardiac death. Increasing evidence indicates the involvement of HERG channels in the pathophysiology of cancer. In the present ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2009.09.010

    authors: Afrasiabi E,Hietamäki M,Viitanen T,Sukumaran P,Bergelin N,Törnquist K

    更新日期:2010-01-01 00:00:00

  • The PI3K effector Arap3 interacts with the PI(3,4,5)P3 phosphatase SHIP2 in a SAM domain-dependent manner.

    abstract::Arap3 is a phosphoinositide (PI) 3 kinase effector that serves as a GTPase activating protein (GAP) for both Arf and Rho G-proteins. The protein has multiple pleckstrin homology (PH) domains that bind preferentially phosphatidyl-inositol-3,4,5-trisphosphate (PI(3,4,5,)P3) to induce translocation of Arap3 to the plasma...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2006.12.015

    authors: Raaijmakers JH,Deneubourg L,Rehmann H,de Koning J,Zhang Z,Krugmann S,Erneux C,Bos JL

    更新日期:2007-06-01 00:00:00

  • Apoptosis induced by cAMP requires Smac/DIABLO transcriptional upregulation.

    abstract::Smac/DIABLO is a mitochondrial protein that participates in apoptotic cell death by means of sequestering several members of the inhibitor of apoptosis protein family. This action allows caspase activation, cleavage of key cellular substrates and death. Release from mitochondria is considered the main regulatory step ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2007.01.001

    authors: Martinez-Velazquez M,Melendez-Zajgla J,Maldonado V

    更新日期:2007-06-01 00:00:00

  • Molecular insights into connective tissue growth factor action in rat pancreatic stellate cells.

    abstract::Pancreatic fibrosis, a key feature of chronic pancreatitis and pancreatic cancer, is mediated by activated pancreatic stellate cells (PSC). Connective tissue growth factor (CTGF) has been suggested to play a major role in fibrogenesis by enhancing PSC activation after binding to alpha5beta1 integrin. Here, we have foc...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2008.06.016

    authors: Karger A,Fitzner B,Brock P,Sparmann G,Emmrich J,Liebe S,Jaster R

    更新日期:2008-10-01 00:00:00

  • Angiotensin II induces nephrin dephosphorylation and podocyte injury: role of caveolin-1.

    abstract::Nephrin, an important structural and signal molecule of podocyte slit-diaphragm (SD), has been suggested to contribute to the angiotensin II (Ang II)-induced podocyte injury. Caveolin-1 has been demonstrated to play a crucial role in signaling transduction. In the present study, we evaluated the role of caveolin-1 in ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2011.09.022

    authors: Ren Z,Liang W,Chen C,Yang H,Singhal PC,Ding G

    更新日期:2012-02-01 00:00:00

  • Early growth response genes 2 and 3 induced by AP-1 and NF-κB modulate TGF-β1 transcription in NK1.1- CD4+ NKG2D+ T cells.

    abstract::NK1.1- CD4+ NKG2D+ T cells are a subpopulation of regulatory T cells that downregulate the functions of CD4+ T, CD8+ T, natural killer (NK) cells, and macrophages through TGF-β1 production. Early growth response genes 2 (Egr2) and 3 (Egr3) maintain immune homeostasis by modulating T lymphocyte development, inhibiting ...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2020.109800

    authors: Han S,Zhu T,Ding S,Wen J,Lin Z,Lu G,Zhang Y,Xiao W,Ding Y,Jia X,Chen H,Gong W

    更新日期:2020-12-01 00:00:00

  • Characteristics of circular RNAs generated by human Survival Motor Neuron genes.

    abstract::Circular RNAs (circRNAs) belong to a diverse class of stable RNAs expressed in all cell types. Their proposed functions include sponging of microRNAs (miRNAs), sequestration and trafficking of proteins, assembly of multimeric complexes, production of peptides, and regulation of transcription. Backsplicing due to RNA s...

    journal_title:Cellular signalling

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellsig.2020.109696

    authors: Ottesen EW,Singh RN

    更新日期:2020-09-01 00:00:00

  • PKCδ stimulates macropinocytosis via activation of SSH1-cofilin pathway.

    abstract::Macropinocytosis is an actin-dependent endocytic mechanism mediating internalization of extracellular fluid and associated solutes into cells. The present study was designed to identify the specific protein kinase C (PKC) isoform(s) and downstream effectors regulating actin dynamics during macropinocytosis. We utilize...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2018.09.018

    authors: Singla B,Lin HP,Ghoshal P,Cherian-Shaw M,Csányi G

    更新日期:2019-01-01 00:00:00

  • Lysyl oxidase-like 2 (LOXL2) controls tumor-associated cell proliferation through the interaction with MARCKSL1.

    abstract::Lysyl oxidase-like 2 (LOXL2) is a member of the lysyl oxidase gene family that contributes to the invasiveness and metastasis in tumor progression. However, the role of LOXL2 in cellular signaling is incompletely understood. In this study, we investigated a possible mechanism of LOXL2 function in tumor metastases in v...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2014.05.010

    authors: Kim BR,Dong SM,Seo SH,Lee JH,Lee JM,Lee SH,Rho SB

    更新日期:2014-09-01 00:00:00

  • Mechanism of induction of muscle protein degradation by angiotensin II.

    abstract::Angiotensin I and II have been shown to directly induce protein degradation in skeletal muscle through an increased activity and expression of the ubiquitin-proteasome proteolytic pathway. This investigation determines the role of the nuclear transcription factor nuclear factor-kappaB (NF-kappaB) in this process. Usin...

    journal_title:Cellular signalling

    pub_type: 杂志文章

    doi:10.1016/j.cellsig.2005.09.009

    authors: Russell ST,Wyke SM,Tisdale MJ

    更新日期:2006-07-01 00:00:00