Abstract:
:M1 macrophages serve one edge as proinflammatory and M2 macrophages serve the other edge as an anti-inflammatory macrophage. It appears that a related "switch" in macrophage morphology may also happen in the course of atherosclerosis, which has not yet been elucidated. An atherogenic diet (AD) was given to rats, and induction of macrophage differentiation and the nuclear localization of nuclear factor-kappa B (NFκB) were investigated by Western blot and immunofluorescence. Chemokines were analyzed using an antibody array with 32 target proteins. M2 macrophage transformation was confirmed in diosgenin-treated aorta by immunofluorescence and was validated in vitro using THP-1 cells. MAC387 (macrophage marker) and NFκBp65 (inflammatory hub) were upregulated in oxidatively-modified low-density lipoprotein (OxyLDL) and AD-induced condition. Macrophage differentiation, which induced the formation of inflammatory mediators, was not significantly suppressed by the inhibition of NFκB using dexamethasone. M1 macrophage polarization was identified in OxyLDL-induced monocytes, which are proinflammatory in nature, whereas M2 macrophage polarization was noticed in diosgenin-treated monocytes, which exhibit anti-inflammatory properties. M1-and M2-specific chemokines were analyzed using chemokine antibody array. Furthermore, the expression of proinflammatory macrophage (M1) was noticed in AD-induced aorta and anti-inflammatory macrophage (M2) was observed in diosgenin-treated aorta. This is the first report where, unifying the mechanism of diosgenin as aan nti-atherosclerotic and the expression of M1 and M2 specific chemokines is shown by downregulating NFκB and not by preventing the differentiation of monocyte into a macrophage, but by allowing macrophage to differentiate into M2, which aids in preventing the atherosclerotic progression.
journal_name
J Biochem Mol Toxicoljournal_title
Journal of biochemical and molecular toxicologyauthors
Binesh A,Devaraj SN,Devaraj Hdoi
10.1002/jbt.22422subject
Has Abstractpub_date
2020-02-01 00:00:00pages
e22422issue
2eissn
1095-6670issn
1099-0461journal_volume
34pub_type
杂志文章abstract::Mitochondria play an important role in myocardial tissue homeostasis; therefore, deterioration in mitochondrial function will eventually lead to cardiomyocyte and endothelial cell death and consequently cardiovascular dysfunction. Lithium (Li+ ) is an effective drug for bipolar disorder with known cardiotoxic side eff...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21836
更新日期:2017-02-01 00:00:00
abstract::Here, we aimed to investigate the carcinogenic effects of apolipoprotein C1 (APOC1) in prostate cancer (PCa). APOC1 expression was evaluated in PCa and normal prostate specimens, and lentivirus-mediated RNA interference was used to knockdown APOC1 in DU145 cells. The effects of APOC1 silencing on cell proliferation, c...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22158
更新日期:2018-05-02 00:00:00
abstract::Casearia esculenta root (Roxb.) is widely used in traditional system of medicine to treat diabetes in India. An active compound, 3-hydroxymethyl xylitol (3-HMX), has been isolated, and its optimum dose has been determined in a short duration study and patented. In addition, the long-term effect of 3-HMX in type 2 diab...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20317
更新日期:2010-03-01 00:00:00
abstract::Certain ginsenosides, also known as triterpene glycosides, have been recently reported to have a characteristic effect on cultured intestinal and leukemia cell growth. Ginsenoside aglycones 20(S)-protopanaxadiol (PD), 20(S)-protopanaxatriol (PT), and ginsenoside Rh2 have been identified as having a strong effect on re...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20019
更新日期:2004-01-01 00:00:00
abstract::Garlic has been used as a traditional medicine to treat various diseases. Garlic reduces the risk of some diseases. This protective effect is due to the organosulfur compounds of garlic. The aim of this study was to investigate the inhibition effects of garlic-derived compounds on human immunodeficiency virus type 1 (...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22215
更新日期:2018-11-01 00:00:00
abstract::Contrary to the fact that doxorubicin is a powerful chemotherapeutic agent for the treatment of neoplastic diseases, cardiotoxicity is too important to be ignored. Thymoquinone serves as a powerful free radical scavenger. In the study, the effects of thymoquinone against doxorubicin-cardiotoxicity will be evaluated. F...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22618
更新日期:2021-01-01 00:00:00
abstract::Cisplatin is a highly cytotoxic anticarcinogenic drug used to treat several kinds of solid tumors such as liver tumors. With the increase in the incidences associated with hepatic tumors and a lack of selectivity of cisplatin to cancer cells, it is important to explore new therapeutic strategies against them. The pres...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22722
更新日期:2021-01-23 00:00:00
abstract::Sodium dichromate [Cr(VI)] and cadmium chloride [Cd(II)] are both cytotoxic and mutagenic. This study examined the toxic and apoptotic potentials of these two cations on three cell types in vitro, namely, human chronic myelogenous leukemic (CML) K562 cells, promyelocytic leukemic HL-60 cells, and normal human peripher...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0461(2000)14:1<33::aid-jbt5>3.0
更新日期:2000-01-01 00:00:00
abstract::We investigated the effects of acute diazepam (DZP) administration on thiobarbituric acid-reactive substance (TBARS) levels, protein carbonyl content, and on the activities of the antioxidant enzymes catalase, glutathione peroxidase, and superoxide dismutase in the brain of rats. Additionally, we investigated the anti...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21815
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:Cadmium is a nonessential toxic heavy metal, which enters the body easily and damages the cellular system. The sonic hedgehog (Shh) signaling pathway is one of the key regulatory pathways, which define neural growth and development. OBJECTIVES:This study aimed to explore how cadmium exposure affects neural ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22274
更新日期:2019-04-01 00:00:00
abstract::The effects of benzo[a]pyrene (B[a]P) on some drug-metabolizing and antioxidant systems in liver, lung, and stomach were investigated in normal and protein malnutrition (PM) rats. PM significantly inhibited tissue glutathione (GSH) content and increased hepatic lipid peroxidation. Cytochrome P450 isoform CYP1A1 was si...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.10064
更新日期:2003-01-01 00:00:00
abstract::Significant changes in drug-metabolizing enzyme (DME) expression occur during ontogeny. Such changes can have a profound effect on therapeutic efficacy in the fetus and child, as well as the risk for adverse drug reactions. To gain a better understanding of DME ontogeny, enzyme contents for six key cytochromes P450 we...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章,评审
doi:10.1002/jbt.20179
更新日期:2007-01-01 00:00:00
abstract::In this work to determine the effects of metals exposure of Oreochromis niloticus on erythrocyte antioxidant systems, fish were exposed to 5.0 mg/L Zn, 1.0 mg/L Cd, and 5.0 mg/L Zn + 1.0 mg/L Cd mixtures for 7 and 14 days and reduced glutathione (GSH) level, catalase (CAT), and glucose-6-phosphate dehydrogenase (G6PD)...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20327
更新日期:2010-07-01 00:00:00
abstract::Carbohydrate-derived aldehydes cause irreversible loss of protein function via glycation. We previously observed that glyceraldehyde 3-phosphate (Glyc3P) abolishes the enzyme activity of cardiac aspartate aminotransferase (cAAT). We also examined the protective effects of carnosine against Glyc3P-induced loss of enzym...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0461(2000)14:4<215::aid-jbt6>3.
更新日期:2000-01-01 00:00:00
abstract::Ochratoxin A (OTA) is nephrotoxic, immunosuppressive, and teratogenic in many species and is a possible human carcinogen. In this study, we investigated the OTA pollution situations of grains in northern China and the signaling pathway that mediated OTA-induced apoptosis in human tubular kidney cells (HKCs). Samples o...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20420
更新日期:2012-03-01 00:00:00
abstract::Minimizing the adverse effects of chemotherapeutic agents remains a therapeutic challenge. Cisplatin (CP) induces hepatotoxicity through activation of oxidative stress, inflammation, and apoptosis cascades which is considered a significant drawback. Thus, this study aimed to highlight the possible hepatoprotective rol...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22714
更新日期:2021-01-25 00:00:00
abstract::This experiment was performed to evaluate the possible embryotoxic and teratogenic effects of doxycycline during rat development. Twenty-one female rats were used and distributed into three groups equally (seven animals/group). The low dose group received doxycycline at a dose of 5 mg/kg bw/day orally from the 6th to ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22613
更新日期:2021-01-01 00:00:00
abstract::Aminoglycoside antibiotics are widely used against Gram-negative infections. On the other hand, nephrotoxicity is a deleterious side effect associated with aminoglycoside therapy. Gentamicin is the most nephrotoxic aminoglycoside. Because of serious health complications ensue the nephrotoxicity induced by aminoglycosi...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21667
更新日期:2015-02-01 00:00:00
abstract::A series of novel indenopyrazole derivatives 2a-j and 3a-j were synthesized from the reaction of 1-(4-(hydroxy(1-oxo-1,3-dihydro-2 H-inden-2-ylidene)methyl)phenyl)-3-phenylurea derivatives 1a-j with hydrazine and phenylhydrazine, respectively. The obtained novel indenopyrazoles ( 2a-j and 3a-j) were evaluated for anti...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22285
更新日期:2019-05-01 00:00:00
abstract::Exposure to arsenic has been linked to the development of type 2 diabetes though its mechanism of toxicity remains unresolved. In this study berberine (BBR) effects on arsenic-induced sirtuin 3 (Sirt3) modifications in isolated mitochondria from rat pancreas were evaluated and compared with metformin (MET). With arsen...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22368
更新日期:2019-09-01 00:00:00
abstract::Diabetes mellitus is a serious worldwide metabolic disease, which is accompanied by hyperglycaemia and affects all organs and body system. Zinc (Zn) is a basic cofactor for many enzymes, which also plays an important role in stabilising the structure of insulin. Liver is the most important target organ after pancreas ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22617
更新日期:2021-01-01 00:00:00
abstract::The objective of this work is to study the protective effects of Quercetin against sodium nitrite-induced hypoxia on liver, lung, kidney and cardiac tissues, also to explore novel mechanism of this compound. Male albino rats were injected with sodium nitrite (75 mg/kg). Quercetin (200 mg kg-1 ,- i.p.) was administrate...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21883
更新日期:2017-05-01 00:00:00
abstract::The present study aims to investigate the protective effect of quercetin against Aroclor-1254-induced hepatotoxicity in rats. Male Wistar rats were grouped into Group I control received vehicle (corn oil; 1 mL/kg bwt); Group II quercetin alone (50 mg/kg bwt/day orally); Group III Aroclor-1254 (2 mg/kg bwt/day intraper...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21466
更新日期:2012-12-01 00:00:00
abstract::This study investigated the protective potential of Naringin (NIN) against cadmium chloride (CdCl2 ) mediated hepatotoxicity using human hepatocellular carcinoma (HepG2) cells. An optimal concentration of NIN (5 μM) was potent enough to confer cytoprotection against CdCl2 (50 μM) as was observed by MTT assay. Precondi...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21915
更新日期:2017-08-01 00:00:00
abstract::Toxicogenomics is a term that represents the merging of toxicology with novel genomics techniques. Data generated in the new-age era of toxicology is relatively complex, requires new bioinformatics tools for adequate interpretation, and allows for the rapid generation of testable hypotheses. Hazard identification and ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章,评审
doi:10.1002/jbt.10006
更新日期:2001-01-01 00:00:00
abstract::In this study, a series of novel bis-sulfone compounds (2a-2j) were synthesized by oxidation of the bis-sulfides under mild reaction conditions. The bis-sulfone derivatives were characterized by 1 H-NMR, 13 C-NMR, Fourier-transform infrared spectroscopy, and elemental analysis techniques. Nuclear Overhauser effect exp...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22401
更新日期:2019-11-01 00:00:00
abstract::Renal xenobiotic transporters are important determinants of urinary secretion and reabsorption of chemicals. In addition to glomerular filtration, these processes are key to the overall renal clearance of a diverse array of drugs and toxins. Alterations in kidney transporter levels and function can influence the effic...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章,评审
doi:10.1002/jbt.21435
更新日期:2012-10-01 00:00:00
abstract::Alkaline phosphatase (ALP) activity in the serum and liver from rats administered with cadmium (Cd) in drinking water was studied. After metal administration, Cd showed a time-dependent accumulation in the liver, meanwhile metallothionein had a maximum increase at 1 month, remaining in this level until the end of the ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21732
更新日期:2015-12-01 00:00:00
abstract::It is well established that crosstalk between cancer-associated fibroblasts (CAFs) and cancer cells plays a critical role in the occurrence and development of oral squamous cell carcinoma (OSCC). The molecular mechanisms underlying such interaction, however, remain far from clear. Accumulating data have indicated that...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21777
更新日期:2016-04-01 00:00:00
abstract::Aldose reductase (AR) is the key enzyme for the polyol pathway and responsible for sorbitol accumulation during the hyperglycemia. The present article focuses on the role of phenol, pyrogallol, hydroquinone, resorcinol, catechol, and phloroglucinol in in vitro inhibition of AR. For this purpose, AR was purified from t...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21935
更新日期:2017-09-01 00:00:00