Abstract:
:Directed evolution (DE) is a powerful approach for generating proteins with new chemical and physical properties. It mimics the principles of Darwinian evolution by imposing selective pressure on a large population of molecules harboring random genetic variation in DNA, such that sequences with specific desirable properties are generated and selected. We propose that combining DE and genome-editing (DE-GE) technologies represents a powerful tool to discover and integrate new traits into plants for agronomic and biotechnological gain. DE-GE has the potential to deliver a new green (r)evolution research platform that can provide novel solutions to major trait delivery aspirations for sustainable agriculture, climate-resilient crops, and improved food security and nutritional quality.
journal_name
Trends Plant Scijournal_title
Trends in plant scienceauthors
Gionfriddo M,De Gara L,Loreto Fdoi
10.1016/j.tplants.2019.08.004subject
Has Abstractpub_date
2019-11-01 00:00:00pages
999-1007issue
11eissn
1360-1385issn
1878-4372pii
S1360-1385(19)30217-1journal_volume
24pub_type
杂志文章,评审abstract::Substantial amounts of nutrients are lost from soils via leaching and as gaseous emissions. These losses can be environmentally damaging and expensive in terms of lost agricultural production. Plants have evolved many traits to optimize nutrient acquisition, including the formation of arbuscular mycorrhizas (AM), asso...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2015.03.004
更新日期:2015-05-01 00:00:00
abstract::Pectin methylesterases catalyse the demethylesterification of cell wall polygalacturonans. In dicot plants, these ubiquitous cell wall enzymes are involved in important developmental processes including cellular adhesion and stem elongation. Here, I highlight recent studies that challenge the accepted views of the mec...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/s1360-1385(01)02045-3
更新日期:2001-09-01 00:00:00
abstract::Meiosis is pivotal in the life history of plants. In addition to providing an opportunity for genetic reassortment, it marks the transition from diploid sporophyte to haploid gametophyte. Recent molecular data suggest that, like animals, plants possess a common set of genes (also conserved in eukaryotic microorganisms...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/s1360-1385(00)01861-6
更新日期:2001-03-01 00:00:00
abstract::The plant fungal pathogen Fusarium oxysporum (Fox) is the causal agent of root rot or wilt diseases in several plant species, including crops such as tomato (Solanum lycopersicum), banana (Musa sapientum) and asparagus (Asparagus officinalis). Colonization of plants by Fox leads to the necrosis of the infected tissues...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2007.12.004
更新日期:2008-03-01 00:00:00
abstract::It is well documented that ancient sickle harvesting led to tough rachises, but the other seed dispersal properties in crop progenitors are rarely discussed. The first steps toward domestication are evolutionary responses for the recruitment of humans as dispersers. Seed dispersal-based mutualism evolved from heavy hu...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2020.01.005
更新日期:2020-04-01 00:00:00
abstract::Higher plants are constructed of three organs--the stem, the root and the leaf. The stem and the root have two axes, apical-basal and central-peripheral, which cross orthogonally. Leaves develop from the shoot apical meristem as lateral organs that have three different axes, apical-basal, adaxial-abaxial and right-lef...
journal_title:Trends in plant science
pub_type: 新闻
doi:10.1016/s1360-1385(01)02155-0
更新日期:2001-12-01 00:00:00
abstract::Gating of K(+) and other ion channels is 'hard-wired' within the channel protein. So it remains a puzzle how closely related channels in plants can show an unusually diverse range of biophysical properties. Gating of these channels lies at the heart of K(+) mineral nutrition, signalling, abiotic and biotic stress resp...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2009.04.001
更新日期:2009-07-01 00:00:00
abstract::Plastid endosymbiosis was accompanied by the appearance of a novel type of semi-cristalline storage polysaccharide (starch). Interestingly, starch is found in the cytoplasm of Rhodophyceae and Glaucophyta but is localized to the chloroplast stroma of Chloroplastida. The pathway is presumed to have been cytosolic in th...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2008.08.009
更新日期:2008-11-01 00:00:00
abstract::Hormesis is a biphasic dose-response relationship with contrasting effects of low versus high doses of stress. Hormesis is rapidly developing in plant science research and has wide implications for risk assessment, stress biology, and agriculture. Here, we explore selected areas of importance to the concept of hormesi...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2020.05.006
更新日期:2020-11-01 00:00:00
abstract::We propose that symbiotically modified organisms (SMOs) should be taken into account in sustainable agriculture. In this opinion article, we present the results of a meta-analysis of the literature, with a particular focus on the potential of SMOs in forage and turf grass production, to determine the impact of endophy...
journal_title:Trends in plant science
pub_type: 杂志文章,meta分析
doi:10.1016/j.tplants.2013.03.003
更新日期:2013-08-01 00:00:00
abstract::Heterosis describes the superior performance of heterozygous hybrid plants over their homozygous parental inbred lines. Despite the rediscovery of this phenomenon a century ago and its paramount agronomic importance, the genetic and molecular basis of heterosis remains enigmatic. Recently, various pioneer studies desc...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2007.08.005
更新日期:2007-09-01 00:00:00
abstract::The Waxy (Wx) gene that governs amylose synthesis is an old but widely used target in improving the quality of starchy crops. New genome-editing strategies are being deployed to create beneficial Wx alleles with finely tuned amylose content (AC). Precise targeting must be combined with traditional approaches to develo...
journal_title:Trends in plant science
pub_type: 杂志文章
doi:10.1016/j.tplants.2020.07.009
更新日期:2020-10-01 00:00:00
abstract::The commercial release of third-generation sequencing technologies (TGSTs), giving long and ultra-long sequencing reads, has stimulated the development of new tools for assembling highly contiguous genome sequences with unprecedented accuracy across complex repeat regions. We survey here a wide range of emerging seque...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2019.05.003
更新日期:2019-08-01 00:00:00
abstract::Plant-derived pharmaceuticals are poised to become the next major commercial development in biotechnology. The advantages they offer in terms of production scale and economy, product safety, ease of storage and distribution cannot be matched by any current commercial system; they also provide the most promising opport...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2005.10.009
更新日期:2005-12-01 00:00:00
abstract::To understand Ca2+ signaling, we need to identify all the Ca2+ transporters and their regulatory components. The first Ca2+ transporters to be cloned from plants and shown to have regulated activity were calmodulin-dependent Ca2+ -pumps. The regulation of these pumps suggests that being able to change the rate of Ca2+...
journal_title:Trends in plant science
pub_type: 新闻
doi:10.1016/s1360-1385(01)02023-4
更新日期:2001-09-01 00:00:00
abstract::Recent studies indicate that a multitude of microbial processes are involved in nitric oxide production and consumptions in the pedosphere. Due to its dual function as a toxic metabolite and signaling compound, we speculate that this pedospheric nitric oxide of microbial origin can significantly interact with mycorrhi...
journal_title:Trends in plant science
pub_type: 杂志文章
doi:10.1016/j.tplants.2020.11.007
更新日期:2021-02-01 00:00:00
abstract::Elucidation of the molecular basis of gene-for-gene interactions between disease-resistance (R) genes and pathogen avirulence (avr) genes has been a Holy Grail of plant pathology for the past decade. Recent studies of the R-avr interaction between RRS1-R and popP2 by Laurent Deslandes et al. provide new insights and s...
journal_title:Trends in plant science
pub_type: 杂志文章
doi:10.1016/j.tplants.2003.11.002
更新日期:2004-01-01 00:00:00
abstract::Plants 'forage' for light in plant canopies using a variety of photosensory systems. Far-red radiation (FR) reflected by neighbours is an early signal of competition that elicits anticipatory shade-avoidance responses. In Arabidopsis and cucumber, perception of reflected FR requires phytochrome B. Horizontal blue (B) ...
journal_title:Trends in plant science
pub_type: 杂志文章
doi:10.1016/s1360-1385(99)01383-7
更新日期:1999-03-01 00:00:00
abstract::Image analysis has become a powerful technique for most plant scientists. In recent years dozens of image analysis tools have been published in plant science journals. These tools cover the full spectrum of plant scales, from single cells to organs and canopies. However, the field of plant image analysis remains in it...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2017.05.002
更新日期:2017-07-01 00:00:00
abstract::Natural genetic variation within plant species is at the core of plant science ranging from agriculture to evolution. Whereas much progress has been made in mapping quantitative trait loci (QTLs) controlling this natural variation, the elucidation of the underlying molecular mechanisms has remained a bottleneck. Recen...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2007.11.008
更新日期:2008-02-01 00:00:00
abstract::Serine/threonine-specific phosphoprotein phosphatases (PPPs) are ubiquitous enzymes in all eukaryotes, but their regulatory functions are largely unknown in higher plants. The Arabidopsis genome encodes 26 PPP catalytic subunits related to type 1, type 2A and so-called novel phosphatases, including four plant-specific...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2007.03.003
更新日期:2007-04-01 00:00:00
abstract::Being continuously exposed to variable environmental conditions, plants produce phytohormones to react quickly and specifically to these changes. The phytohormone ethylene is produced in response to multiple stresses. While the role of ethylene in defense responses to pathogens is widely recognized, recent studies in ...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2018.01.003
更新日期:2018-04-01 00:00:00
abstract::Current agricultural practice depends on a wide use of pesticides, bactericides, and fungicides. Increased demand for organic products indicates consumer preference for reduced chemical use. Therefore, there is a need to develop novel sustainable strategies for crop protection and enhancement that do not rely on genet...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2015.01.004
更新日期:2015-04-01 00:00:00
abstract::A range of bio- and nanotechnologies have been developed that could be adapted towards monitoring the presence and expression of transgenes, in real time and in the field, in plants of agronomic and ecological importance. Transgene escape from crop hosts to wild relatives or landraces is one example in which monitorin...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2005.06.003
更新日期:2005-08-01 00:00:00
abstract::The secretory pathway encloses functionally interlinked organelles for the synthesis and deposition of most of the building blocks of eukaryotic cells, such as lipids, proteins and sugars. The coat protein complex II (COPII) is a specialized protein complex for the transport between secretory organelles, specifically ...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2010.05.010
更新日期:2010-09-01 00:00:00
abstract::Serine (Ser) has a fundamental role in metabolism and signaling in living organisms. In plants, the existence of different pathways of Ser biosynthesis has complicated our understanding of this amino acid homeostasis. The photorespiratory glycolate pathway has been considered to be of major importance, whereas the non...
journal_title:Trends in plant science
pub_type: 杂志文章
doi:10.1016/j.tplants.2014.06.003
更新日期:2014-09-01 00:00:00
abstract::The study of insular systems has a long history in ecology and biogeography. Island plants often differ remarkably from their noninsular counterparts, constituting excellent models for exploring eco-evolutionary processes. Trait-based approaches can help to answer important questions in island biogeography, yet plant ...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2019.12.022
更新日期:2020-04-01 00:00:00
abstract::Plants have mechanisms to promote outbreeding and thereby to increase their genetic diversity. In species that are self-incompatible, self-pollen is rejected by the stigma. This mechanism has been the subject of intense study for many years and, in the past two years, significant progress has been made in identifying ...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/s1360-1385(00)01759-3
更新日期:2000-10-01 00:00:00
abstract::Proteins often act in complexes assembled via protein-protein interaction domains. The sterile alpha motif (SAM) domain is one of the most prominent interaction domains in animals and is present in proteins of diverse functions. This domain allows head-to-tail closed oligomerisation or polymer formation resulting in h...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/j.tplants.2017.06.006
更新日期:2017-08-01 00:00:00
abstract::To exploit plants as living substrates, biotrophic fungi have evolved remarkable variations of their tubular cells, the hyphae. They form infection structures such as appressoria, penetration hyphae and infection hyphae to invade the plant with minimal damage to host cells. To establish compatibility with the host, co...
journal_title:Trends in plant science
pub_type: 杂志文章,评审
doi:10.1016/s1360-1385(02)02297-5
更新日期:2002-08-01 00:00:00