Abstract:
:The amount of H antigens on buccal epithelial cells (BEC) of secretors and non-secretors was measured by flow cytometry. H type 1 and H type 2 on BEC were detected with fluorescein-labelled Ulex europaeus lectin, and H type 2 was measured with mouse monoclonal antibody. Between 3.5 and 6 times more H antigen was detected on cells from secretors compared with cells from non-secretors. The level of H type 2 was the same on BEC of secretors and non-secretors. Adsorption of H type 1 antigen from secretor saliva onto non-secretors BEC was demonstrated. A simple and sensitive ELISA method was developed for the determination of H in saliva with biotin-labelled Ulex lectin. This system provides a simple means for measuring the amount of H present in saliva and for the determination of secretor status.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Rahat A,Stewart J,Blackwell CC,Weir DMdoi
10.1111/j.1423-0410.1990.tb05019.xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
101-5issue
2eissn
0042-9007issn
1423-0410journal_volume
59pub_type
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