Semi-quantitative determination of H type 1 and type 2 antigens on buccal epithelial cells and in saliva of secretors and non-secretors.

Abstract:

:The amount of H antigens on buccal epithelial cells (BEC) of secretors and non-secretors was measured by flow cytometry. H type 1 and H type 2 on BEC were detected with fluorescein-labelled Ulex europaeus lectin, and H type 2 was measured with mouse monoclonal antibody. Between 3.5 and 6 times more H antigen was detected on cells from secretors compared with cells from non-secretors. The level of H type 2 was the same on BEC of secretors and non-secretors. Adsorption of H type 1 antigen from secretor saliva onto non-secretors BEC was demonstrated. A simple and sensitive ELISA method was developed for the determination of H in saliva with biotin-labelled Ulex lectin. This system provides a simple means for measuring the amount of H present in saliva and for the determination of secretor status.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Rahat A,Stewart J,Blackwell CC,Weir DM

doi

10.1111/j.1423-0410.1990.tb05019.x

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

101-5

issue

2

eissn

0042-9007

issn

1423-0410

journal_volume

59

pub_type

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