Abstract：
INTRODUCTION:Bioscaffolds derived from animal organs are promising materials for xenotransplantation and regenerative medicine. For effective generation of biological scaffolds from diverse organs, there have been many technical challenges. In this study, we introduced a novel approach to create multiorgan bioscaffolds through systemic decellularization. METHODS AND MATERIALS:To obtain acellular bioscaffolds, the healthy adult rats were systemically perfused with ionic detergent through the carotid artery. Additional liver perfusion was set up to prevent potential obstruction from the influx of the decellularized debris via the portal vein. The perfusion system was controlled to maintain a constant physiological cardiac output of approximately 50 mL/min and was designed to minimize air entrapment. After decellularization, every organ designated for bioscaffold was harvested for evaluation of vascular structure and histology. RESULTS:The perfusion times were different for each organ. In our histological analysis, the decellularized bioscaffolds harvested from most organs including major solid organs (ie, heart, liver, and kidney) as well as the others (such as stomach, intestines, spleen, etc) represented no evidence of residual cellular materials. Furthermore, the well-preserved collagen materials and intact vascular structures were also confirmed. CONCLUSION:The results from this study suggested that this systemic decellularization has the advantages to obtain a variety of bioscaffolds from single donor, and we can even decellularize organs with complex influx vascular structures. This method may also be used to study organ bioengineering for patients who need simultaneous combined organ transplantation.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Park KM,Woo HMdoi
10.1016/j.transproceed.2012.03.017subject
Has Abstractpub_date
2012-05-01 00:00:00pages
1151-4issue
4eissn
0041-1345issn
1873-2623pii
S0041-1345(12)00237-0journal_volume
44pub_type
杂志文章abstract：:This study examined the combinatory effect on graft survival of neonatal pig pancreatic cell clusters (NPCC) with nordihydroguaiaretic acid (NDGA), a 5-lipoxygenase inhibitor, with systemic CTLA4Ig expression, with local CTLA4Ig and with interleukin-1 (IL-1) receptor antagonist (IL-1ra) expression using a pig to mouse...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.10.089
更新日期：2006-12-01 00:00:00
abstract：:The 2 main indications for emergency liver transplantation are severe acute hepatic insufficiency and emergency retransplantation. In Spain, since the creation of the National Transplant Organisation (NTO), known as "the Spanish model," there have been high rates of donation, with a mean of 33.9 donors per million inh...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.09.131
更新日期：2005-11-01 00:00:00
abstract：:In September 2001, the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) founded the Collaborative Islet Transplant Registry (CITR). Each year, CITR produces a complete set of analyses available to the public. In its second year, CITR represents the efforts of 19 North American islet transplant ...
journal_title：Transplantation proceedings
pub_type： 杂志文章,多中心研究
doi：10.1016/j.transproceed.2006.10.014
更新日期：2007-01-01 00:00:00
abstract：AIM:This study investigated changes in the mucosal barrier of transplanted intestines with particular emphasis on antigen sampling by Peyer's patches (PPs). METHODS:Heterotopic small bowel transplantation (SBTx) was performed as described previously. C57BL/6 mice were used as donors and BALB/c (allogeneic) or C57BL/6 ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.08.057
更新日期：2010-11-01 00:00:00
abstract：BACKGROUND:To evaluate the diagnostic accuracy of intravoxel incoherent motion (IVIM) parameters in estimation of hepatocellular carcinoma (HCC) grading. MATERIALS AND METHODS:Twenty-nine patients with histopathologically diagnosed as 42 HCC at explant were included in this retrospective study. All patients were exami...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.02.027
更新日期：2019-01-01 00:00:00
abstract：:Liver transplantation for chronic HBV-induced cirrhosis is associated with a high rate of recurrence and poor long-term survival. Prolonged and combined prophylaxis with hepatitis B immunoglobulin (HBIg) and lamivudine has been demonstrated to prevent HBV recurrence, but its lifelong administration is highly expensive...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/s0041-1345(03)00633-x
更新日期：2003-08-01 00:00:00
abstract：BACKGROUND:Kidney transplantation is known to increase the survival of dialysis patients by ameloriating cardiac status, including both systolic and diastolic functions. We aimed to evaluate the role of immunosuppressive drug regimens on cardiac functions of kidney transplant recipients (KTRs). METHODS:We prospectivel...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.02.007
更新日期：2017-04-01 00:00:00
abstract：INTRODUCTION:An important side effect of sirolimus, a drug often used in organ transplantation, is pulmonary toxicity. MATERIALS AND METHODS:We present five kidney transplant patients who developed this toxicity associated with sirolimus. All underwent chest radiography computed tomography, fiberoptic bronchoscopy wit...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.08.037
更新日期：2006-10-01 00:00:00
abstract：PURPOSE:We aim to see the rate of progression to chronic kidney disease stage III after living donor nephrectomy in a single institution annually. METHODS:Between May 2006 and July 2017, a total of 753 living kidney donors who were followed up more than 6 months were enrolled in the study. We divided normal function v...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.03.058
更新日期：2019-10-01 00:00:00
abstract：:Ipsilateral acute iliofemoral deep vein thrombosis (DVT) immediately after kidney transplantation is rare but highly morbid, resulting in allograft failure, rupture, or even death. Treatment modalities for iliofemoral DVT occurring just after transplantation are limited due to bleeding risk and impaired renal function...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.05.041
更新日期：2011-07-01 00:00:00
abstract：AIM:To investigate depressive symptoms in recipients of renal transplantation (RTx) compared with patients undergoing hemodialysis (HD) in China. METHODS:Forty-two Chinese recipients of transplant and 42 Chinese patients on hemodialysis were randomly selected from 2011 to 2012. These 2 groups were assessed with the va...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.07.029
更新日期：2018-12-01 00:00:00
abstract：:Urologic complications are common in renal transplant surgery. Numerous innovations have been developed to circumvent ureterovesical anastomotic failure. In addition to the popular modified Lich-Gregoir technique, we evaluated Taguchi's method which is both quick and easy to perform. One hundred forty four patients we...
journal_title：Transplantation proceedings
pub_type： 临床试验,杂志文章
doi：10.1016/j.transproceed.2005.08.006
更新日期：2005-09-01 00:00:00
abstract：:Total incidence and outcome of cancer in all renal allograft recipients in one geographic area are known: 126 (7%) of 1884 patients developed cancer during an 11-year period. Proportions with cancer 1 and 5 years following successful transplantation were 11% and 24%, respectively. Numbers, proportion of malignancies, ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：BACKGROUND:The influence of recombinant human prolactin (rh-PRL) added to Biolasol solution (concentration 1 μg/L) on selected markers (pH, osmolarity, Na(I) and K(I) concentration) and enzymatic activity (alanine aminotransferase [ALT], aspartate aminotransferase [AST], and lactate dehydrogenase [LDH]) in perfundates ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2015.12.140
更新日期：2016-06-01 00:00:00
abstract：INTRODUCTION:Our previous reports suggested that African Americans (AA) are more likely to develop end-stage renal disease (ESRD) following kidney donation when compared with white counterparts. We sought information on age, gender, and race of kidney donors to determine which groups were over-represented on the kidney...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.03.104
更新日期：2008-06-01 00:00:00
abstract：:We report a case of living related renal transplantation that used the recipient's saphenous vein as a graft to extend the length of the right donor renal vein. A 41-year-old woman underwent ABO-incompatible living related renal transplantation from her 74-year-old mother in November 2014. A retroperitoneal laparoscop...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.03.089
更新日期：2018-10-01 00:00:00
abstract：:Cyclosporine absorption has been key to obtaining adequate results in immunosuppressive regimens. Since 2005, we have used a different monitoring program for Cyclosporine among pediatric liver transplant recipients namely, two hours post dosing concentrations (O(2)). This study of 128 patients included 50.8% males and...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.11.016
更新日期：2010-01-01 00:00:00
abstract：INTRODUCTION:Intestinal manipulation leads to local bowel wall inflammation that subsequently spreads over the entire gastrointestinal tract. Previously, this gastrointestinal field effect had been demonstrated by us in a rodent model. We herein postulated an immunologic mechanism mediated by activated leukocytes. The ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.06.087
更新日期：2006-07-01 00:00:00
abstract：:Among 71 patients, 19 (26.7%) experienced tacrolimus-related complications including 15 neurologic reactions and four problems with nephrotoxicity. Seven of these patients received grafts from cadaveric donors and 12 from living donors. Nine patients were children. The cohort included 5 female and 14 male subjects of ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.12.114
更新日期：2006-03-01 00:00:00
abstract：:A 27-year-old Japanese man underwent liver transplantation because of uncompensated cirrhosis due to Dorfman-Chanarin syndrome (DCS). At birth, the patient displayed ichthyosis and liver dysfunction. Moreover, mental retardation appeared and intracytoplasmic vacuoles were observed within peripheral blood neutrophils. ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.07.105
更新日期：2010-11-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) plays a critical role in transplant rejection. Herein we addressed whether HGF expression could be used for accurate and early diagnosis of acute and chronic rejection in cardiac transplantation. We used a heterotopic cardiac transplantation model using nonhuman primates (Macaca fuscata,...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.10.075
更新日期：2006-12-01 00:00:00
abstract：BACKGROUND:From 1968 until 1997, transplantation-related activities were not properly regulated and were informally practiced. During 20 years, many legal and political changes influenced it. OBJECTIVE:To provide a historical overview of the 20 years with a descriptive data analysis of a 20-year data set. METHODOLOGY...
journal_title：Transplantation proceedings
pub_type： 历史文章,杂志文章
doi：10.1016/j.transproceed.2020.01.075
更新日期：2020-06-01 00:00:00
abstract：INTRODUCTION:The occurrence of thrombocytopenia in the perioperative period after a liver transplant is not uncommon. However, there are few studies on persistent thrombocytopenia during the longer follow up period of patients after liver transplantation. We examined the prevalence of and contributing factors to persis...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.07.001
更新日期：2004-10-01 00:00:00
abstract：BACKGROUND:Primary pulmonary hypertension can lead to hypertrophy of the right ventricle and ultimately to its insufficiency. Lung transplantation remains the only viable treatment for patients with certain forms of this disease. Usage of extracorporeal membrane oxygenation in veno-arterial form (VA-ECMO) after transpl...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2020.02.113
更新日期：2020-09-01 00:00:00
abstract：:Episodes of acute rejection may represent an important risk factor for the development of chronic allograft nephropathy. Various studies have shown that pretransplant cytokine profiles in recipient blood are associated with transplant outcome. Serum samples were collected 24 hours before transplantation from 57 patien...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.08.031
更新日期：2005-09-01 00:00:00
abstract：BACKGROUND:An extended-release formulation of tacrolimus designed for once-daily administration (LCP-TAC) is a new prolonged-release tacrolimus (TAC-PR) formulation using a drug delivery technology designed to enhance the bioavailability of drugs compared with TAC-PR. The aim of this study was to retrospectively compar...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.03.084
更新日期：2019-11-01 00:00:00
abstract：OBJECTIVES:The human major histocompatibility complex class I chain-related antigen A, B (MICA, B) are believed to be "cell stress sensors," which encode stress-inducible surface proteins that bind to NKG2D, an activating receptor of NK, alpha beta and gamma delta T cells. While cyclosporine (CsA) has improved patient ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.06.080
更新日期：2006-09-01 00:00:00
abstract：:Downstaging treatment of hepatocellular carcinoma (HCC) prior to liver transplant (LT) is an accepted strategy to meet the Milan criteria. However, after transplant surgery, a reality is noted that the number or/and the size of some HCCs measured from the liver explants is different from that measured from the pre-LT ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.03.020
更新日期：2019-06-01 00:00:00
abstract：BACKGROUND:The aim of this study was to examine which demographic and comorbidity factors affected the activation of patients with end-stage renal disease on the national kidney transplantation waiting list. METHODS:This was a prospective cohort study across 13 transplantation centers in the United Kingdom from Octobe...
journal_title：Transplantation proceedings
pub_type： 杂志文章,多中心研究
doi：10.1016/j.transproceed.2013.03.033
更新日期：2013-07-01 00:00:00
abstract：BACKGROUND:Mycophenolate mofetil (MMF) is an effective immunosuppressive agent that has been frequently used in laboratory animals including swine; however, the pharmacokinetic properties of MMF in swine have not been studied. This short-term study was designed to evaluate the feasibility and the pharmacokinetic profil...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.08.040
更新日期：2014-12-01 00:00:00
