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A Laboratory Strain of Leishmania major: Protective Effects on Experimental Leishmaniasis.

Abstract：

PURPOSE:Leishmaniasis, as one of the most important vector-borne and zoonotic diseases, can be seen in different forms and is more prevalent in developing countries worldwide. Due to the absence of effective strategies in its prevention, treatment, and control, investigation of effective control strategies against the disease is necessary. In this research, we evaluated the immunogenicity of a cold-adapted laboratory strain of Leishmania major (LMC) in the mouse model. METHODS:Twenty BALB/c mice were divided into two groups. LMC group received 4 × 106 of LMC strain in 0.5 ml DMEM, and VLM group, as the control group, received 0.5 ml Dulbecco's modified Eagle's medium. Both groups were challenged with virulent L. major 3 weeks after inoculation. RESULTS:The data obtained from the analysis of immune responses and histopathological changes interestingly revealed protection against L. major in immunized mice. Compared with the VLM group, the mice immunized with LMC strain of L. major in the LMC group showed a significant increase in IFN-γ and IgG2a levels (P < 0.05) which are important indexes for Th1-related immune responses. Additionally, significant differences in concentration of IgG1 and IgG total before and after the challenge was observed in LMC group (P < 0.05). Furthermore, the immunized mice showed a significant reduction in mean sizes of skin lesion and liver damage compared to the VLM group. CONCLUSION:Based on the present findings on immunogenicity of LMC strain, it seems this strain is able to induce both humoral and cellular immunity and a significant protection against L. major in the mouse model.

journal_name

Acta Parasitol

journal_title

Acta parasitologica

authors

Namavari M,Namazi F,Asadi-Manesh R,Hosseini MH,Nazifi S,Asadpour M

doi

10.2478/s11686-019-00068-2

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

645-651

issue

3

eissn

1230-2821

issn

1896-1851

pii

10.2478/s11686-019-00068-2

journal_volume

64

pub_type

杂志文章

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