Abstract:
:In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19/260) and 5.8% (15/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6/260) and 1.5% (4/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.
journal_name
Acta Parasitoljournal_title
Acta parasitologicaauthors
Liu J,Guan G,Liu A,Li Y,Yin H,Luo Jdoi
10.2478/s11686-014-0222-6subject
Has Abstractpub_date
2014-03-01 00:00:00pages
132-8issue
1eissn
1230-2821issn
1896-1851journal_volume
59pub_type
杂志文章abstract::Giardia duodenalis is an ubiquitous flagellate that infects humans and many species of animals. This species exhibits great biotypic and genetic diversity. In the present study, we established short- and long-term in vitro cultures of G. duodenalis trophozoites originating from red deer and Thomson's gazelle (artiodac...
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更新日期:2020-12-01 00:00:00
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更新日期:2015-06-01 00:00:00
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更新日期:2013-03-01 00:00:00
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更新日期:2013-12-01 00:00:00
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更新日期:2015-09-01 00:00:00
abstract::A high infestation of the monogenean Gussevia tucunarense in a cultivation of bujurqui-tucunare was reported. The prevalence was 100%. The mean intensity and abundance of the parasite was 164.4 of parasites per individual. This is the first report of a high infestation by G. tucunarense in C. semifasciatus cultured fr...
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更新日期:2013-06-01 00:00:00
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更新日期:2019-06-01 00:00:00
abstract::A new feather mite species, Trouessartia sechellarum sp. n. (Astigmata: Analgoidea: Trouessartiidae), is described from the Seychelles magpie-robin Copsychus sechellarum (Passeriformes: Muscicapidae), an endangered endemic passerine bird inhabiting the Seychelles Islands. The most clear features distinguishing this mi...
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