Vaccination with a cocktail of Ancylostoma ceylanicum recombinant antigens leads to worm burden reduction in hamsters.

Abstract:

:Hookworms, a group to which Ancylostoma ceylanicum belongs, are gastrointestinal nematodes that infect more than 700 million people around the world. They are a leading cause of anemia in developing countries. In order to effectively prevent hookworm infections research is conducted to develop an effective vaccine using recombinant antigens of the parasite. The aim of this study was to examine the influence of the hosts' on protection against ancylostomiasis and the shaping of the humoral immune response among Syrian hamsters after immunization with a cocktail of five A. ceylanicum recombinant antigens. Ace-ASP-3, Ace-ASP-4, Ace-APR-1, Ace-MEP-6 and Ace-MEP-7 were obtained in the pET expression system. Immunization with a vaccine cocktail resulted in a 33.5% worm burden reduction. The immunogenicity of the recombinant proteins were determined using ELISA. Statistical analysis showed that vaccinated hamsters developed stronger humoral responses to four of five recombinant antigens (the exception being Ace-ASP-3) compared to hamsters from the control group.

journal_name

Acta Parasitol

journal_title

Acta parasitologica

authors

Wiśniewski M,Łapiński M,Daniłowicz-Luebert E,Jaros S,Długosz E,Wędrychowicz H

doi

10.1515/ap-2016-0074

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

556-61

issue

3

eissn

1230-2821

issn

1896-1851

pii

/j/ap.2016.61.issue-3/ap-2016-0074/ap-2016-0074.xm

journal_volume

61

pub_type

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