Abstract:
:Hookworms, a group to which Ancylostoma ceylanicum belongs, are gastrointestinal nematodes that infect more than 700 million people around the world. They are a leading cause of anemia in developing countries. In order to effectively prevent hookworm infections research is conducted to develop an effective vaccine using recombinant antigens of the parasite. The aim of this study was to examine the influence of the hosts' on protection against ancylostomiasis and the shaping of the humoral immune response among Syrian hamsters after immunization with a cocktail of five A. ceylanicum recombinant antigens. Ace-ASP-3, Ace-ASP-4, Ace-APR-1, Ace-MEP-6 and Ace-MEP-7 were obtained in the pET expression system. Immunization with a vaccine cocktail resulted in a 33.5% worm burden reduction. The immunogenicity of the recombinant proteins were determined using ELISA. Statistical analysis showed that vaccinated hamsters developed stronger humoral responses to four of five recombinant antigens (the exception being Ace-ASP-3) compared to hamsters from the control group.
journal_name
Acta Parasitoljournal_title
Acta parasitologicaauthors
Wiśniewski M,Łapiński M,Daniłowicz-Luebert E,Jaros S,Długosz E,Wędrychowicz Hdoi
10.1515/ap-2016-0074subject
Has Abstractpub_date
2016-09-01 00:00:00pages
556-61issue
3eissn
1230-2821issn
1896-1851pii
/j/ap.2016.61.issue-3/ap-2016-0074/ap-2016-0074.xmjournal_volume
61pub_type
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