Abstract:
:Opportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (AIDS). We investigated the existence of opportunistic protozoans that significantly affect the quality of life in HIV-1 infected patients using conventional and molecular methods. The study group comprised 115 HIV-1 positive patients. In the identification of Cyclospora cayetanensis and Cryptosporidium, the formol-ether precipitation method was used and smears were evaluated in optical microscope by staining modified Ziehl-Neelsen (ZN). The primers and probes used for PCR were Heat shock protein 70 for C. cayetanensis and the oocysts wall protein for Cryptosporidium spp.. Cyclospora and Cryptosporidium spp. oocysts were detected in one and two patients, respectively, by staining, whereas we detected C. cayetanensis in three patients out of 115 (2.6%) by PCR, and Cryptosporidium spp. in a further three patients (2.6%). C. cayetensis was detected in patients with CD4 counts of 64 cells/μm, 182 cells/μm and 287 cells/μm, respectively. Cryptosporidium spp. was detected in patients with CD4 counts of 176 cells/μm, 241 cells/μm and 669 cells/μm. As conclusion, PCR method is faster and more sensitive than microscopic methods and to screen intestinal pathogens routinely in patients infected with HIV should not be neglected in developing countries like Turkey.
journal_name
Acta Parasitoljournal_title
Acta parasitologicaauthors
Uysal HK,Adas GT,Atalik K,Altiparmak S,Akgul O,Saribas S,Gurcan M,Yuksel P,Yildirmak T,Kocazeybek B,Ziver T,Oner YAdoi
10.1515/ap-2017-0067subject
Has Abstractpub_date
2017-09-26 00:00:00pages
557-564issue
3eissn
1230-2821issn
1896-1851pii
/j/ap.2017.62.issue-3/ap-2017-0067/ap-2017-0067.xmjournal_volume
62pub_type
杂志文章abstract::Giardia duodenalis is an ubiquitous flagellate that infects humans and many species of animals. This species exhibits great biotypic and genetic diversity. In the present study, we established short- and long-term in vitro cultures of G. duodenalis trophozoites originating from red deer and Thomson's gazelle (artiodac...
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journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-014-0270-y
更新日期:2014-09-01 00:00:00
abstract:BACKGROUND:Giardia duodenalis is a widespread protozoan parasite affecting humans and many species of animals, including dogs and cats. Due to its zoonotic potential, it is important to know the frequency of this parasite in companion animals. The aim of this study was to determine current epidemiological status of G. ...
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更新日期:2020-10-11 00:00:00
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更新日期:2019-09-01 00:00:00
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更新日期:2017-03-01 00:00:00
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更新日期:2020-03-01 00:00:00
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更新日期:2020-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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pub_type: 杂志文章
doi:10.2478/s11686-013-0168-0
更新日期:2013-12-01 00:00:00
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doi:10.1515/ap-2015-0096
更新日期:2015-12-01 00:00:00
abstract:PURPOSE:Larval stages of trombiculid mites infest a wide variety of wild and domestic animals. The most common clinical signs related with the presence of these parasites are alopecia, crusts, erythema, excoriation, erosion, papules, pustules and vesicles. Most of trombiculid infestations may not be perceived by the cl...
journal_title:Acta parasitologica
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doi:10.1007/s11686-020-00247-6
更新日期:2020-07-15 00:00:00
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doi:10.1515/ap-2016-0074
更新日期:2016-09-01 00:00:00
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journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-013-0181-3
更新日期:2013-12-01 00:00:00
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journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-020-00202-5
更新日期:2020-09-01 00:00:00
abstract::An in vitro study has been carried out to monitor changes to the female reproductive system in adult triclabendazole (TCBZ)-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh ("Mirazid"). Flukes were immersed for 6 h and 24 h in myrrh extract at a concentration of 200 µg/ml, then pr...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.1515/ap-2017-0041
更新日期:2017-06-01 00:00:00
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pub_type: 杂志文章
doi:10.2478/s11686-013-0129-7
更新日期:2013-06-01 00:00:00
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doi:10.2478/s11686-018-00006-8
更新日期:2019-03-01 00:00:00
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更新日期:2016-01-01 00:00:00
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journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.1515/ap-2017-0077
更新日期:2017-09-26 00:00:00
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journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-019-00039-7
更新日期:2019-12-01 00:00:00
abstract::Larval trematodes infecting the snail Heleobia australis (Cochliopidae) from the Bahía Blanca estuary, Argentina were surveyed for two years. A total of 7,504 snail specimens was dissected and the larval stages of 15 different trematodes were recovered and examined morphologically. These larvae included four species t...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-014-0209-3
更新日期:2014-03-01 00:00:00
abstract::During July 2011, a single Cordillera striped shrew-rat (Chrotomys whiteheadi) was collected from the Philippines and its faeces examined for coccidian parasites. It harboured an eimerian that we describe here as new. Oocysts of Eimeria macarthuri sp. n. were spheroidal to subspheroidal with a bi-layered wall and meas...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-014-0304-5
更新日期:2014-10-01 00:00:00
abstract::This paper characterizes the pattern of ectoparasite and endoparasite communities in an assemblage of 35 sympatric fish from different trophic levels in a tributary from the Amazon River system, northern Brazil. In detritivorous, carnivorous, omnivorous and piscivorous hosts, the species richness consisted of 82 ectop...
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pub_type: 杂志文章
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更新日期:2018-06-26 00:00:00
abstract::Despite their tremendous diversity and their medical and veterinary importance, details of egg ultrastructure among the digenean trematodes has been studied rather little. The available literature is spread over several decades and several species, but has not been adequately reviewed to reveal patterns of similarity ...
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pub_type: 杂志文章,评审
doi:10.1515/ap-2018-0001
更新日期:2018-03-26 00:00:00
abstract::Two new species of Oswaldocruzia, O. manuensis sp. nov., and O. urubambaensis sp. nov. are described and illustrated from Peru, these are parasites of the cane toad Rhinella marina. O. manuensis is characterized by having cervical alae which are not well developed, ridges without chitinous supports, caudal bursa type ...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-013-0103-4
更新日期:2013-03-01 00:00:00
abstract::Microsporidiosis is considered to be emerging opportunistic infection in immunocompromised individuals worldwide. The aim of this study was to identify the specific serum antibodies to intestinal microsporidia Encephalitozoon cuniculi and Encephalitozoon intestinalis in women with Human Papillomavirus HPV and without ...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.1515/ap-2015-0007
更新日期:2014-03-01 00:00:00
abstract::High performance thin-layer chromatography was used to determine the concentration of β-carotene and lutein in the whole body and digestive gland-gonad complex (DGG) of uninfected Biomphalaria glabrata snails and those infected with Schistosoma mansoni for 6 and 8 weeks. Pigments were extracted from the snails using a...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.2478/s11686-013-0140-z
更新日期:2013-09-01 00:00:00
abstract::A new species of dajid isopod in the genus Aspidophryxus G.O. Sars, 1883 is described from three females and one male found infesting the lateral carapace of Heteromysis (Olivemysis) actiniae Clarke, 1955 (Mysidae: Heteromysinae) associated with the corkscrew anemone Bartholomea annulata (Lesuer, 1817) (Anthozoa: Aipt...
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pub_type: 杂志文章
doi:10.2478/s11686-012-0052-3
更新日期:2012-12-01 00:00:00