Abstract:
BACKGROUND:The aim of the present study is to assess the osteogenic differentiation of rat bone marrow stromal cells (RBMCs) in β-tricalcium phosphate (β-TCP) blocks containing extracellular matrix (ECM) produced by human alveolar bone periosteal cells (HABPCs). METHODS:HABPCs were cultured in β-TCP blocks for 1 week (group 1) and 2 weeks (group 2). β-TCP blocks containing ECM were then created by drying the blocks for 3 days. RBMCs were cultured in the blocks containing ECM for 2 weeks. In the control group, RBMCs were cultured in β-TCP blocks alone for 2 weeks. HABPCs and RBMCs in the blocks were examined by histologic and immunohistochemical analyses. RESULTS:Histology revealed a significantly higher number of HABPCs in the group 2 blocks than in the group 1 blocks. HABPCs produced several bone matrix proteins in the blocks, and these positive reactions in group 2 were significantly higher than in group 1. Both groups showed a significantly higher number of RBMCs than the control group. RBMCs produced osteopontin and osteocalcin in the blocks, and these positive reactions were significantly higher in both test groups than in the control group. The number of osteocalcin-positive reactions was higher in group 2 than in group 1. CONCLUSION:Osteogenic differentiation of RBMCs cultured for 2 weeks in β-TCP blocks containing ECM was significantly higher than that of cells cultured for 1 week or without ECM.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Iwasaki K,Munemasa N,Tago K,Nemoto K,Tsujigami H,Sugaya A,Deguchi Sdoi
10.1902/jop.2012.120490subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1484-92issue
10eissn
0022-3492issn
1943-3670journal_volume
84pub_type
杂志文章abstract::The ability to objectively assess periodontal disease activity can significantly affect periodontal therapy. Aspartate aminotransferase (AST) is released extracellularly upon tissue destruction which suggests its potential as a key index in a quantitative assay for evaluating periodontal disease activity. The purpose ...
journal_title:Journal of periodontology
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doi:10.1902/jop.1996.67.8.733
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journal_title:Journal of periodontology
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journal_title:Journal of periodontology
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1993.64.2.110
更新日期:1993-02-01 00:00:00
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journal_title:Journal of periodontology
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pub_type: 杂志文章
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 杂志文章,meta分析,评审
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1986.57.12.746
更新日期:1986-12-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1994-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/JPER.17-0705
更新日期:2019-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2003.74.11.1634
更新日期:2003-11-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1996.67.8.803
更新日期:1996-08-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2007.060402
更新日期:2007-06-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1996.67.10.1004
更新日期:1996-10-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2004.75.1.91
更新日期:2004-01-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1996.67.3.271
更新日期:1996-03-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.17-0670
更新日期:2018-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2003.74.10.1447
更新日期:2003-10-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2015.140490
更新日期:2015-10-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-09-01 00:00:00