Abstract:
BACKGROUND:A specific labeler of the human gingival fibroblast (HGF) does not exist. Anionic maghemite nanoparticles allow labeling of a wide cell variety and their recognition in cellular, organotypical, and animal models. METHODS:We studied internalization effects of nanoparticles on an HGF phenotype in vitro, evaluating transcription and secretion of connective tissue remodeling molecules, i.e., matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and cytokines controlling their activation/inhibition, i.e., transforming growth factor-beta (TGF-beta1), tumor necrosis factor-alpha (TNF-alpha), and interleukins 1beta and 4 (IL-1beta and IL-4). After proliferation kinetics, cellular uptake was studied by Perls coloration and magnetophoresis on labeled culture. Dot blotting, Western blotting, and zymography were used to detect MMP-1, -2, and -3 and TIMP-1 and -2 secretions in culture supernatants, and reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the mRNA expression of these molecules. Enzyme-linked immunosorbent assay (ELISA) tests were used to determine TGF-beta1, TNF-alpha, IL-1beta, and IL-4 levels. RESULTS:Our data indicated high (15.3+/-5.8 pg/cell) but heterogeneous distribution of nanoparticles in HGF. Twenty-four hours after labeling, MMP-1, -2, and -3 and TIMP-2 secretion increased (P<0.001) with RT-PCR confirmation at 12 hours, whereas TIMP-1 did not. IL-1beta increased at day 1 (D1) (P<0.001) and IL-4 at D3 (P<0.01), but not TGF-beta1 or TNF-alpha. CONCLUSIONS:After labeling with these maghemite nanoparticles, HGF increased secretion of IL-1beta at D1, probably inducing the increase of MMP-1, -2, and -3 and TIMP-2. The increase of IL-4 secretion began with the decreased synthesis of MMPs and TIMPs at D3. Despite this transitory inflammatory reaction at 3 days following internalization, maghemite nanoparticles did not affect HGF phenotype, thereby authorizing their use as labelers.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Naveau A,Smirnov P,Ménager C,Gazeau F,Clément O,Lafont A,Gogly Bdoi
10.1902/jop.2006.050064subject
Has Abstractpub_date
2006-02-01 00:00:00pages
238-47issue
2eissn
0022-3492issn
1943-3670journal_volume
77pub_type
杂志文章abstract:BACKGROUND:Several risk indicators for periodontal disease severity have been identified. The association of these factors with tooth loss for periodontal reasons was investigated in this cross-sectional comparative study. METHODS:All extractions performed in 21 general dental practice clinics (25% of such clinics in ...
journal_title:Journal of periodontology
pub_type: 杂志文章,多中心研究
doi:10.1902/jop.2005.76.11.1910
更新日期:2005-11-01 00:00:00
abstract:BACKGROUND:The pathobiology of systemic lupus erythematosus (SLE) is similar to that of periodontitis in that the immunoglobulin G Fc receptor (FcgammaR) and proinflammatory cytokines play an important role. Genetic variations of FcgammaR and interleukin (IL)-1 are associated with susceptibility to both diseases. There...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2007.060194
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:Coronally advanced flap (CAF) has been shown to effectively treat gingival recession. Platelet-rich plasma (PRP), containing autologous growth factors, has been shown to promote soft tissue healing. Therefore, the purpose of this study was to evaluate the effects of PRP in combination with CAF. METHODS:Twen...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2005.76.10.1768
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:Mucous membrane pemphigoid is a heterogeneous group of autoimmune, subepithelial, blistering diseases. A combination of topical and systemic steroid treatment is often used when managing patients with mucous membrane pemphigoid. The use of systemic steroids presents an increased risk of adverse side effects....
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.100095
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:Previous studies have shown an association between a specific genotype for the inflammatory cytokine interleukin (IL)-1 and the severity of periodontal disease. The purpose of this study was to evaluate the effect of the IL-1 genotype on the outcomes of periodontal surgical regenerative treatment with bone r...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2004.75.10.1335
更新日期:2004-10-01 00:00:00
abstract::A case of osseous hyperplasia under the pontics of fixed partial dentures in right and left mandibular first molar regions is presented. Radiographs showed hemispherical radio-opacities on the alveolar ridges. Histologic examination revealed the lesions were composed of a dense mass of mature bone with well-developed ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.5.311
更新日期:1988-05-01 00:00:00
abstract:BACKGROUND:Subantimicrobial-dose doxycycline (SDD) is widely used as an adjunctive treatment to scaling and root planing (SRP), but its long-term effectiveness remains controversial. The purpose of this systematic review was to assess the actual evidence of the effectiveness of SRP + SDD compared to SRP + placebo in th...
journal_title:Journal of periodontology
pub_type: 杂志文章,meta分析,评审
doi:10.1902/jop.2011.110026
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:The development of chronic periodontitis (CP) is a multifactorial process and variation in severity cannot be explained by just a few risk factors. The aims of this study were: 1) to explore the lifestyle and psychosocial factors of CP patients and 2) to estimate the proportion of total CP cases attributable...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2003.74.8.1169
更新日期:2003-08-01 00:00:00
abstract::In recent years there has been a dramatic increase in the use of nonprecious alloy and porcelain crowns in clinical dentistry. The alloy in these restorations frequently contains a high percentage (greater than 70%) of nickel. Most cases of metal hypersensitivity are related to nickel, and clinical manifestations of t...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1987.58.7.486
更新日期:1987-07-01 00:00:00
abstract::The distance from cemento-enamel junction to alveolar crest was studied in relation to periodontal treatment need, socioeconomic factors and dental health. The subjects (315) of the study were employees of a paper mill in Finland. Alveolar crest height was measured from orthopantomograms, periodontal treatment need wa...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1981.52.2.99
更新日期:1981-02-01 00:00:00
abstract:BACKGROUND:The comparative treatment response of children and young adults with localized aggressive periodontitis treatment (LAgP) affecting primary and permanent dentition is unknown. The objective of this study is to evaluate the influence of non-surgical periodontal therapy with adjunctive systemic antibiotics on t...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
doi:10.1902/jop.2014.140171
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:Serum amyloid A (SAA) has been identified to trigger inflammation response, and play a crucial role in chronic inflammatory diseases. However, the regulatory mechanism of SAA still remains unclear during the development of periodontitis METHODS: SAA mRNA and protein expression were detected in healthy and in...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.19-0050
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Lingual plate perforation at the time of implant placement in posterior mandible is a potential surgical complication, and presence of a lingual concavity is considered a risk factor. Little is known about the spatial relationship between implant and lingual plate. The role of lingual concavity in the risk o...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.100313
更新日期:2011-01-01 00:00:00
abstract::The relationship between diabetes mellitus and oral health status was determined in Pima Indians from the Gila River Indian Community in Arizona. This tribe of native Americans has the world's highest reported incidence and prevalence of non-insulin-dependent (type 2) diabetes mellitus. The probing attachment level, a...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1991.62.2.123
更新日期:1991-02-01 00:00:00
abstract:BACKGROUND:In early case studies, use of a collagen barrier as a guided tissue regeneration (GTR) material has shown particular promise in procedures aimed at root coverage. The similarities between collagen membrane and subepithelial connective tissue graft (SCTG) have made collagen membrane an attractive and a possib...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.2001.72.10.1301
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND:Non-surgical and surgical periodontal treatment may cause volumetric changes in the gingival contour. Many techniques have been suggested to minimize soft tissue shrinkage; however, there is a lack of three-dimensional (3D) quantitative data comparing different treatment approaches. The aim of the present st...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080288
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:The aim of this in vivo study was to evaluate the effects of plaque control on the patency of dentinal tubules using vital teeth of beagle dogs. METHODS:Class V cavities were prepared on the cervical areas of the mandibular and maxillary molars in each dog with a diamond point. To simulate the state of dent...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.050159
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:Controversy over the long-term clinical effectiveness of hydroxyapatite (HA)-coated dental implants still persists, despite numerous clinical studies documenting high survival rates. Concerns about the degradation of the coating over the years have been raised; it has been speculated that resorption of the H...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2007.060271
更新日期:2007-05-01 00:00:00
abstract::Healing of extraction sockets where the buccal plate of bone has been destroyed often results in localized ridge deformities. This is most likely due to the ingrowth of extraskeletal connective tissue into the space originally occupied by the tooth which then fills with a combination of bone and soft tissue. Technique...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1994.65.1.17
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:The biologic safety profile of oral health care products is often assumed on the basis of simplistic test models such as monolayer cell culture systems. We developed and characterized a tissue-engineered human oral mucosal model, which was proven to represent a potentially more informative and more clinicall...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080610
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Surgical crown lengthening has been proposed as a means of facilitating restorative procedures and preventing periodontal injuries in teeth with structurally inadequate clinical crowns or exposing tooth structure in the presence of deep, subgingival pathologies which may hamper the access for proper restorat...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2001.72.7.841
更新日期:2001-07-01 00:00:00
abstract::Multiple periodontal lesions were the presenting features leading to a diagnosis of multiple myeloma in a 38-year-old male. Ulceration and bleeding from a rapidly growing retromolar myelomatous mass, multiple foci of alveolar bone destruction and cortical expansion together with multifocal skeletal destruction were th...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1990.61.2.132
更新日期:1990-02-01 00:00:00
abstract:BACKGROUND:Osteoblasts and periodontal ligament stem cells (PDLSCs) play an important role in maintaining physiologic function of periodontal tissues and participating in periodontal regeneration. Elucidation of interactions between osteoblasts and PDLSCs will aid understanding of periodontal regeneration mechanisms. T...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2017.170016
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Various modifications of the laterally sliding flap have been proposed to reduce the risk of gingival recession at the donor tooth site, but the reported root coverage predictability was quite low. The goal of the present study was to evaluate the effectiveness with respect to root coverage of a modified sur...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2004.75.12.1734
更新日期:2004-12-01 00:00:00
abstract::Examiner diagnostic accuracy and response times were compared using subtraction and conventional radiography in a dog model. Osseous lesions were induced interproximally and through the mandibular buccal bone interradicularly and radicularly. Standardized preoperative and postoperative conventional radiographs of the ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1985.56.6.324
更新日期:1985-06-01 00:00:00
abstract:BACKGROUND:Systemic bone loss has been proposed as a risk factor for periodontal disease; however, the relationship between these two diseases is still not clear. The objective of this study was to assess the relationship between systemic bone mineral density and periodontal disease, controlling for known confounders. ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2000.71.9.1492
更新日期:2000-09-01 00:00:00
abstract:BACKGROUND:Lysine, a nutritionally essential amino acid, enters the oral cavity in gingival crevicular fluid (GCF). During oral hygiene restriction (OHR), lysine decarboxylase (LDC) in dento-gingival biofilms converts lysine to cadaverine. Lysine depletion impairs the dental epithelial barrier to bacterial proinflammat...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2015.140490
更新日期:2015-10-01 00:00:00
abstract::The capacity of epithelial cells to migrate and grow from gingival explants cultured on mineralized and partially demineralized root surfaces in an in vitro system was assessed. Explants of attached gingiva were obtained from mongrel dogs, cut into rectangular pieces (1 x 2 mm) and cultured either on mineralized or pa...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.8.531
更新日期:1988-08-01 00:00:00
abstract:BACKGROUND:Implants replacing missing teeth provide advantages over clinical orthodontic treatment as compensation for reaction forces is no longer necessary and the lack of teeth is immediately resolved. METHODS:A total of 38 two-stage implants were inserted (16 in maxilla, 22 in mandible) in 10 partially edentulous ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2004.75.1.176
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:It has been shown that toll-like receptor (TLR) 2- and TLR4-stimulating abilities of supragingival plaque (SPP) are associated with periodontal conditions. It is hypothesized that SPP might affect the periodontium through its influence on subgingival plaque (SBP). This study investigates relationships betwee...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2016.150573
更新日期:2016-09-01 00:00:00