Abstract：
:CALX, the NCX homolog in Drosophila, involves in light-mediated Ca(2+) homeostasis in sensory neuronal cells. CALX exhibits a unique negative Ca(2+) regulatory property mediated by Ca2+ binding at its intracellular regulatory domain. Our structural studies of individual CBD1 or CBD2 domain reveal that CBD1 is the only Ca(2+) binding domain in CALX. Crystal structures of the entire Ca(2+) regulatory domain CBD12 from two alternative splicing isoforms, CALX1.1 and CALX1.2, demonstrate that CBD1 and CBD2 form an open V-shaped conformation with four Ca(2+) ions bound on the CBD domain interface. The structures together with Ca(2+) binding analyses strongly argue that the Ca(2+) inhibition of CALX is achieved by interdomain conformational change induced by Ca(2+) binding at CBD1. The conformational difference between the two isoforms also raises a hypothesis that alternative splicing residues adjust the interdomain orientation angle between CBD1 and CBD2 to modify the Ca(2+) regulatory property of the exchanger. These studies not only establish structural basis to understand the inhibitory Ca(2+) regulation and the alternative splicing modification of CALX, but also shed light on the general Ca(2+) regulatory mechanism of other mammalian NCX proteins.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Zheng L,Wu M,Tong Sdoi
10.1007/978-1-4614-4756-6_6subject
Has Abstractpub_date
2013-01-01 00:00:00pages
55-63eissn
0065-2598issn
2214-8019journal_volume
961pub_type
杂志文章,评审abstract：:Immunohistochemical localizations of calcineurin subunits A (60 KDa) and B (20 KDa) were examined in a rat brain using subunit specific monoclonal antibodies. The immunoreactivity of the subunit A was abundant in the hippocampus, in the striatum, and in the thalamus, but weak in the neocortex. On the contrary, the imm...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5679-0_40
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abstract：:The blood-brain barrier (BBB) is the rate-limiting step in the translation of neurotrophin neuroscience into clinically effective neurotherapeutics. Since neurotrophins do not cross the BBB, these proteins cannot be used for neuroprotection following intravenous administration, and it is not feasible to administer the...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4615-0123-7_15
更新日期：2002-01-01 00:00:00
abstract：:In Poland, influenza surveillance is conducted by 16 Provincial Sanitary Epidemiological Stations, headed by the Department of Influenza Research, National Influenza Center at the National Institute of Health - National Institute of Hygiene (NIPH-NIH). During the epidemic season 2016/2017, more than 4000 samples were ...
journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：1995-01-01 00:00:00
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更新日期：2010-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 临床试验,杂志文章
doi：10.1007/978-1-4757-0154-8_22
更新日期：1986-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5143-6_33
更新日期：1986-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-4-431-56847-6_10
更新日期：2018-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4863-8_71
更新日期：1998-01-01 00:00:00
abstract：:The mammalian genome is believed to contain some 30,000 to 40,000 different genes. Of these an estimated 42% have no known function. Genetically engineered mouse models (GEMM) have been a powerful tool available for determining gene function in vivo. In the mammary gland, a variety of genetic engineering approaches ha...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4757-4242-8_20
更新日期：2004-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4020-5133-3_20
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pub_type： 杂志文章,评审
doi：10.1007/978-1-60761-500-2_11
更新日期：2010-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-1899-0_88
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-8616-2_14
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pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-1825-3_216
更新日期：1997-01-01 00:00:00
abstract：:The cloning of the MEN1 gene in 1997 led to the characterization of menin, the protein behind the multiple endocrine neoplasia Type 1 syndrome. Menin, a novel nuclear protein with no homology to other gene products, is expressed ubiquitously. MEN1 missense mutations are dispersed along the coding region of the gene bu...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4419-1664-8_3
更新日期：2009-01-01 00:00:00
abstract：:Recently advances in cancer genomics revealed the unexpected high frequencies of epigenetic abnormalities in human acute myeloid leukemia (AML). Accumulating data suggest that these leukemia-associated epigenetic factors play critical roles in both normal hematopoietic stem cells (HSCs) and leukemia stem cells (LSCs)....
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pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4419-7756-4_19
更新日期：2011-01-01 00:00:00
abstract：:Recent studies suggested that colorectal cancer influences the types and quantity of nucleic acids - especially microRNAs - detected in the bloodstream. Concentration of circulating (cell-free) microRNAs, and possibly of other non-coding RNAs, could therefore serve as valuable colorectal cancer biomarker and could del...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-42059-2_9
更新日期：2016-01-01 00:00:00
abstract：:Zebrafish are increasingly being used to study cancer. Almost all tumor types have been found in zebrafish. However, tumor incidence is relatively low and tumors develop late in life. Functional inactivation of tumor suppressors is a crucial step in cancer progression and more and more tumor suppressor genes are being...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-30654-4_4
更新日期：2016-01-01 00:00:00
