Abstract：
:A series of native and chemically derivatized lactoferrins (Lfs) purified from milk and colostrum were assayed in vitro for their anti-HIV and anti-HCMV-cytopathic effects in MT4 cells and fibroblasts respectively. All Lfs from bovine and human milk or colostrum were able to completely block HCMV replication as well as inhibited HIV-1 induced cytopathic effects. Through acylation of the amino function of the lysine residues in Lf, using anhydrides of succinic acid or cis-aconitic acid, negatively charged Lf derivatives were obtained that all showed a strong antiviral activity against the HIV-1 in vitro. Acylated-Lf exhibited a 4-fold stronger antiviral effect on HIV-1 than the parent compound but the activity on HCMV was abolished. Peptide scanning studies indicated that the native Lf as well as acylated Lf strongly bind to the V3 domain of the HIV envelope protein gp120, with Kd values in the same concentration range as the in vitro IC50. Therefore, shielding of this domain, resulting in inhibition of the virus-cell fusion and entry of the virus in MT4 cells is the likely mechanism underlying the anti-HIV activity. In contrast, addition of positive charges to Lf through amination of the proteins resulted in an increased anti-HCMV activity and a loss of anti-HIV activity, with anti-HCMV IC50 values in the low micromolar concentration range. The N-terminal portion of LF appeared essential to this anti-HCMV effect. The specific distribution of positively and negatively charged domains in the molecule appears to be important in both the anti-HIV and anti-HCMV effects.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Swart PJ,Kuipers EM,Smit C,Van Der Strate BW,Harmsen MC,Meijer DKsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
205-13eissn
0065-2598issn
2214-8019journal_volume
443pub_type
杂志文章abstract：:One of the key abnormalities of non-insulin-dependent diabetes mellitus (NIDDM) and related diseases of the "Metabolic Syndrome" is impaired insulin action (insulin resistance). Since skeletal muscle plays a major role in insulin-stimulated glucose uptake and whole-body energy expenditure, it is a central player in ca...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4899-1928-1_12
更新日期：1998-01-01 00:00:00
abstract：:The P300 event-related potential (ERP) is associated with attention and memory operations of the brain. P300 is changed in many cognitive disorders such as dementia, Alzheimer, schizophrenia, and major depression disorder. Therefore, investigation on basis of this component can help to improve our understanding of pat...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4419-7046-6_60
更新日期：2011-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:1. Rat skeletal muscle can synthesize purine nucleotides de novo. 2. Nucleotide synthesis de novo was inhibited by approximately 90% in the presence of 1 mM azaserine. 3. Purine nucleotides can be synthesized via "salvage" pathways from purine bases and nucleosides. Under the conditions employed incorporation rates we...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-8559-2_3
更新日期：1979-01-01 00:00:00
abstract：:Various members of the respiratory chain exhibit different optical properties in the reduced and oxidized forms, thus enabling the non-invasive monitoring of various organs in vitro as well as in vivo. Since the pioneering work of Chance, Cohen, Jobsis and Schoener in 1962, many groups of investigators adopted their a...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-9510-6_43
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abstract：:We conducted an epidemiological survey of antibiotic resistance in Salmonella recovered from market-age swine at five different Texas farms. These farms, which were visited between October 1997 and June 1998, were completely integrated, farrow-to-finish operations. Samples were taken from the lymph nodes and cecal con...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4143-1_31
更新日期：1999-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-3-319-29073-7_11
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-3426-6_17
更新日期：1992-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-11093-2_1
更新日期：2019-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-94-007-4549-0_29
更新日期：2013-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-1280-2_26
更新日期：1987-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4737-2_20
更新日期：1999-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-0205-0_35
更新日期：2003-01-01 00:00:00
abstract：:In this article we discuss the pathophysiology of common symptoms of acute viral respiratory infections (e.g., sneezing, nasal discharge, sore throat, cough, muscle pains, malaise, and mood changes). Since clinical symptoms are not sufficient to determine the etiology of viral respiratory tract infections, we believe ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/5584_2015_110
更新日期：2015-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Increasing evidence has shown that mesenchymal stem cells (MSCs) isolated from body tissues are heterogeneous while being examined in vitro and in vivo. Besides some common characteristics, MSCs derived from different tissues exhibit unique biological properties. In addition, the therapeutic effects of MSCs may vary w...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-11096-3_10
更新日期：2019-01-01 00:00:00
abstract：:Insulin secretion in humans is usually induced by mixed meals, which upon ingestion, increase the plasma concentration of glucose, fatty acids, amino acids, and incretins like glucagon-like peptide 1. Beta-cells can stay in the off-mode, ready-mode or on-mode; the mode-switching being determined by the open state prob...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-12457-1_37
更新日期：2020-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-6012-4_3
更新日期：1991-01-01 00:00:00
abstract：:An innate immune system capable of distinguishing among self, non-self, and danger is a prerequisite for health. Upon antigenic challenge, pattern recognition receptors (PRRs), such as the Toll-like receptor (TLR) family of proteins, enable this system to recognize and interact with a number of microbial components an...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-0-387-74087-4_7
更新日期：2008-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-9182-2_69
更新日期：1993-01-01 00:00:00
abstract：:Breathing vitally serves body homeostasis. The prevalence of upper airway infections is often taken as an indicator of overall health status of a population living at a given time. In the present study we examined the unearthed remains of skulls from the XIII-XV century inhabitants searching for signs of maxillary sin...
journal_title：Advances in experimental medicine and biology
pub_type： 历史文章,杂志文章
doi：10.1007/978-94-007-6627-3_20
更新日期：2013-01-01 00:00:00
abstract：:Compared to controls receiving physiological saline, the i.p. administration of dimethylnitrosamine (DMN) on 5 consecutive days to rats fed a nutritionally adequate liquid diet resulted 24 hours after the last injection of significant increases in glutamic dehydrogenase (GDH), glutamic oxylacetate transaminase (GOT), ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-1419-7_25
更新日期：1980-01-01 00:00:00
abstract：:1. The age and sex distribution of the SUA of the Japanes people was the same as of the Caucasians. 2. The prevalence of gout was 0.4% of all the subjects, in adult male it was 1.2% and 76.4% in hyperuricemia above 10.0 mg/dl. 3. The incidence of a year throughout this population study was 0.02% per population. 4. Ser...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-9140-5_25
更新日期：1980-01-01 00:00:00
abstract：:Skin is the main organ that covers the human body and acts as a protective barrier between the human body and the environment. Skin tissue as a stem cell source can be used for transplantation in therapeutic application in terms of its properties such as abundant, easy to access, high plasticity and high ability to re...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/5584_2019_380
更新日期：2020-01-01 00:00:00
abstract：:Xenogeneic immunization of freshly-prepared human glioma extracts into goats has yielded a polyclonal antiserum, which after multiple absorptions specifically identifies antigenic entities only in glioma extracts, and not in appropriate controls, both by radioimmunoassays (RIAs) and Western immunoblots. The results fr...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-6000-1_30
更新日期：1991-01-01 00:00:00
abstract：:Bacteriophage play many varied roles in microbial ecology and evolution. This chapter collates a vast body of knowledge and expertise on Yersinia pestis phages, including the history of their isolation and classical methods for their isolation and identification. The genomic diversity of Y. pestis phage and bacterioph...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-94-024-0890-4_13
更新日期：2016-01-01 00:00:00
abstract：:Hypoxic pulmonary vasoconstriction (HPV) is an important mechanism by which localized flow of blood in small resistance pulmonary arteries is matched to alveolar ventilation. This chapter discusses the role of several potassium and calcium channels in HPV, both in enhancing calcium influx into smooth muscle cells (SMC...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-60761-500-2_1
更新日期：2010-01-01 00:00:00
abstract：:Concurrent complex sounds (e.g., two voices speaking at once) are perceptually disentangled into separate "auditory objects". This neural processing often occurs in the presence of acoustic-signal distortions from noise and reverberation (e.g., in a busy restaurant). A difference in periodicity between sounds is a str...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-25474-6_41
更新日期：2016-01-01 00:00:00
abstract：:Bezoar Bovis (BB:dried cattle gallbladder stones) has been used empirically in Asia for over 3000 years to treat heart and liver disorders. Yet its therapeutic potential remains unexplored by Western researchers. The aim of this study has been to clarify the actions of BB on cultured cardiomyocytes and to identify its...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-0-387-75681-3_10
更新日期：2009-01-01 00:00:00
