Abstract：
:Xenogeneic immunization of freshly-prepared human glioma extracts into goats has yielded a polyclonal antiserum, which after multiple absorptions specifically identifies antigenic entities only in glioma extracts, and not in appropriate controls, both by radioimmunoassays (RIAs) and Western immunoblots. The results from the absorbed polyclonal antiserum have been confirmed by the successful generation of six stable murine monoclonal antibodies (MAbs) which recognize a subset of the same antigens with high specificity on immunoblots and with no apparent cross-reactivities by RIA to normal brain, serum, liver, muscle, kidney, spleen, or melanoma tissues. Moreover, the tested murine MAbs (B12C4) reveal a striking and abundant glial filament protein, possibly related to glial fibrillary acidic protein (GFAP) or other intermediate filament proteins, by frozen-section immunofluorescence. This is seen only in gliomas and is absent, or dramatically reduced, in normal human cortex. Use of potent immortalizing strain (FF41) of Epstein-Barr virus (EBV) to establish antibody-secreting human lymphoblastoid lines, and the generation of mouse-human chimeric fusions, have yielded lines possessing variable supernatant human antibody secretion. Radioimmunoassays using culture supernatants, and sera from glioma patients and an normal individual, have demonstrated surprisingly similar reactivity profiles, even after a sensitive sandwich RIA employing the B6C6 murine MAb. These results suggest that, although human glioma-associated antigens, including possibly the up-regulation of GFAP expression, clearly exist, there seems to be a muted humoral response as evidenced by a paucity of tumor-specific B-cells. This may be due to antigenic shielding by the blood-brain barrier, or due to a form of immunological compromise in patients harboring these malignancies.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Fischer DK,Matsuda M,Shaban F,Narayan RK,Atassi MZdoi
10.1007/978-1-4684-6000-1_30subject
Has Abstractpub_date
1991-01-01 00:00:00pages
263-70eissn
0065-2598issn
2214-8019journal_volume
303pub_type
杂志文章abstract：:Studies of high-altitude populations, and in particular of maladapted subgroups, may provide important insight into underlying mechanisms involved in the pathogenesis of hypoxemia-related disease in general. Chronic mountain sickness (CMS) is a major public health problem in mountainous regions of the world affecting ...
journal_title：Advances in experimental medicine and biology
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abstract：:Immunotherapy entails the treatment of disease by modulation of the immune system. As detailed in the previous chapters, the different modes of achieving immune modulation are many, including the use of small/large molecules, cellular therapy, and radiation. Oncolytic viruses that can specifically attack, replicate wi...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-94-017-7555-7_5
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abstract：:Skin is the main organ that covers the human body and acts as a protective barrier between the human body and the environment. Skin tissue as a stem cell source can be used for transplantation in therapeutic application in terms of its properties such as abundant, easy to access, high plasticity and high ability to re...
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abstract：:While clinical guidelines clearly define mechanisms for asthma diagnosis based upon history, lung function testing, symptoms, and physical examination, surveillance for asthma is much less straightforward. Epidemiologists have long debated the best means of assessing the scope and burden of asthma, seeking to reduce t...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4614-8603-9_2
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4419-0298-6_4
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/5584_2017_19
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4615-1857-0_3
更新日期：1995-01-01 00:00:00
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更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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更新日期：2010-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-37639-0_8
更新日期：2020-01-01 00:00:00
abstract：:An important metabolite of ceramide is ceramide-1-phosphate (C1P). This lipid second messenger was first demonstrated to be mitogenic for fibroblasts and macrophages and later shown to have antiapoptotic properties. C1P is also an important mediator of the inflammatory response, by stimulating the release of arachidon...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2010-01-01 00:00:00
abstract：:Here, we have demonstrated potent inhibition of allergen-mediated pulmonary inflammation and airway hyper-responsiveness by a novel dual-pronged action of LX's. Moreover, these results indicate that LX's play pivotal and previously unappreciated roles in regulating allergy and pulmonary inflammation. ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4419-9194-2_5
更新日期：2003-01-01 00:00:00
