Abstract：
:To isolate pancreatic progenitor cells with the surface markers of hematopoietic stem cells, the expression of stem cell antigen (Sca-1) and c-Kit and the coexpression of them with pancreatic duodenal homeobox-1 (PDX-1), neurogenin 3 (Ngn3), and insulin were examined in murine embryonic pancreas. Then different pancreatic cell subpopulations were isolated by magnet-activated cell sorting. Isolated cells were cultured overnight in hanging drops. When cells formed spheres, they were laid on floating filters at the air/medium interface. With this new culture system, pancreatic progenitor cells were induced to differentiate to endocrine and exocrine cells. It was shown that c-Kit and Sca-1 were expressed differently in embryonic pancreas at 12.5, 15.5, and 17.5 days of gestation. The expression of c-Kit and Sca-1 was the highest at 15.5 days of gestation. c-Kit rather than Sca-1 coexpressed with PDX-1, Ngn3, and insulin. Cells differentiated from c-Kit-positive cells contained more insulin-producing cells and secreted more insulin in response to glucose stimulation than that from c-Kit-negative cells. These results suggested that c-Kit could be used to isolate pancreatic progenitor cells and our new culture system permitted pancreatic progenitor cells to differentiate to mature endocrine cells.
journal_name
Int J Endocrinoljournal_title
International journal of endocrinologyauthors
Ma F,Chen F,Chi Y,Yang S,Lu S,Han Zdoi
10.1155/2012/948683subject
Has Abstractpub_date
2012-01-01 00:00:00pages
948683eissn
1687-8337issn
1687-8345journal_volume
2012pub_type
杂志文章abstract：BACKGROUND:Recent guidelines from the American Thyroid Association (ATA) indicate that, in many patients affected by differentiated thyroid cancer (DTC), the serum TSH should be maintained between 0.1 and 0.5 mU/L. The purpose of this study was to evaluate the TSH variability of patients affected by DTC treated with li...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2017/7053959
更新日期：2017-01-01 00:00:00
abstract：:Background. Liraglutide (a glucagon-like peptide 1 analog) was used for the treatment of type 2 diabetes (T2DM) which could produce glucose-dependent insulin secretion. Aim. The aim was to investigate whether liraglutide could improve myofibril and mitochondria injury in skeletal muscle and the mechanisms in diabetic ...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2014/312452
更新日期：2014-01-01 00:00:00
abstract：:Background. Oxidative stress induced by reactive oxygen and nitrogen species is critically involved in the impairment of β -cell function during the development of diabetes. Methods. HIT-T15 cells were cultured in 5% CO2 and then preincubated with Gelam honey extracts (20, 40, 60, and 80 µg/mL) as well as quercetin (2...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2013/367312
更新日期：2013-01-01 00:00:00
abstract：:Background. Papillary thyroid cancer (PTC) exhibits a higher incidence in women. Due to various ages at menarche and menopause, estrogen levels vary, which may account for the differences in the occurrence, development, and prognosis of female patients with PTC. Objective. The aim of this study was to investigate the ...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2017/5702716
更新日期：2017-01-01 00:00:00
abstract：Objective:Many patients with diabetes opt to fast for lab tests, especially for lipid profiles, thus missing breakfast. In parallel, recent studies and international guidelines have indicated that routine fasting for lipid panels may not be necessary. Missing breakfast while fasting for lab tests may invoke hypoglycemi...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2018/1528437
更新日期：2018-10-24 00:00:00
abstract：Objective:Aim of this study is to evaluate determinants of secondary normocalcemic hyperparathyroidism (SNHPT) persistence in patients who have undergone Roux-en-Y gastric bypass on vertical-banded gastroplasty. Methods:226 consecutive patients submitted to bariatric surgery were prospectively enrolled and divided in ...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2018/5010287
更新日期：2018-03-06 00:00:00
abstract：:In obese diabetic subjects, a correct life style, including diet and physical activity, is part of a correct intervention protocol. Thus, the aim of this study was to evaluate the effects of aerobic training intervention, based on heart rate at aerobic gas exchange threshold (AerTge), on clinical and physiological par...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2015/695297
更新日期：2015-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2017/2549491
更新日期：2017-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2013/580685
更新日期：2013-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2019/5731639
更新日期：2019-05-16 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章,评审
doi：10.1155/2020/1385138
更新日期：2020-05-25 00:00:00
abstract：:Vascular calcification and bone fragility are common and interrelated health problems that affect chronic kidney disease (CKD) patients. Bone fragility, which leads to higher risk of fracture and mortality, arises from the abnormal bone remodeling and mineralization that are seen in chronic kidney disease. Recently, s...
journal_title：International journal of endocrinology
pub_type： 杂志文章,评审
doi：10.1155/2018/5893514
更新日期：2018-04-02 00:00:00
abstract：:Objective. To examine the association of three most common obesity measures including body mass index (BMI), waist circumference (WC), and waist-to-hip ratio (WHR) with chronic kidney disease (CKD) risk in patients with type 2 diabetes mellitus (T2D). Design. Cross-sectional evaluation of the effect of anthropometric ...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2015/715871
更新日期：2015-01-01 00:00:00
abstract：:Anovulatory cycles and endometriosis are the main causes of female infertility. The most frequently anovulatory cycles are related to polycystic ovary syndrome (PCOS) commonly associated with obesity and hormonal disturbances in the course of obesity. Recently published studies revealed that infertility affects about ...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2012/236217
更新日期：2012-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2015/168047
更新日期：2015-01-01 00:00:00
abstract：:Aim. To compare two creatinine-based estimated glomerular filtration rate (eGFR) equations, the chronic kidney disease epidemiology collaboration (CKD-EPI) and the modification of diet in renal disease (MDRD), for predicting the risk of CKD progression in type 2 diabetic patients with nephropathy. Methods. A total of ...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2013/848963
更新日期：2013-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2020/6302861
更新日期：2020-02-21 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章,评审
doi：10.1155/2015/534320
更新日期：2015-01-01 00:00:00
abstract：:Purpose. We investigate the usefulness of multimodal assistant systems using a fusion model of preoperative three-dimensional (3D) computed tomography (CT) and magnetic resonance imaging (MRI) along with endoscopy with indocyanine green (ICG) fluorescence in establishing endoscopic endonasal transsphenoidal surgery (E...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2015/694273
更新日期：2015-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2014/768506
更新日期：2014-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章,评审
doi：10.1155/2015/294278
更新日期：2015-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2015/957105
更新日期：2015-01-01 00:00:00
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journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2020/2080797
更新日期：2020-04-27 00:00:00
abstract：Background:The aim was to investigate the amounts of saliva and serum asprosin in order to determine whether it is related to obesity and whether salivary glands synthesize asprosin or not. Methods:A total of 116 underweight, normal weight, overweight, and obese (class I, class II, and class III) volunteers participat...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2019/2521096
更新日期：2019-03-27 00:00:00
abstract：:The hypothalamus is an essential relay in the neural circuitry underlying energy metabolism that needs to continually adapt to changes in the energetic environment. The neuroendocrine control of food intake and energy expenditure is associated with, and likely dependent upon, hypothalamic plasticity. Severe disturbanc...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2012/732975
更新日期：2012-01-01 00:00:00
abstract：:A quarter of men with obesity or type 2 diabetes have hypogonadotropic hypogonadism. Animal studies and in vitro data have shown that insulin action and insulin responsiveness in the brain are necessary for the maintenance of the functional integrity of the hypothalamo-hypophyseal-gonadal axis. We conducted a randomiz...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2018/6170154
更新日期：2018-10-31 00:00:00
abstract：Objective:Selenium, as an antioxidant, has been implicated in the development of autoimmune thyroiditis (AIT). Many studies showed selenium supplementation could decrease thyroid autoantibodies in patients with AIT. However, the underlying mechanisms have not been well determined. Therefore, we performed a clinical stu...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2020/9210572
更新日期：2020-06-30 00:00:00
abstract：Background:Type 2 diabetes mellitus is a serious public health problem worldwide. Accumulating evidence has shown that β-cell dysfunction is an important mechanism underlying diabetes mellitus. The changes in the physiological state of islet stellate cells (ISCs) and the effects of these cells on β-cell dysfunction is ...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2020/4708132
更新日期：2020-02-24 00:00:00
abstract：:Iodine supplementation through salt iodization is a worldwide, effective strategy for preventing iodine deficiency-related problems. Its safety and efficacy profile has been extensively investigated, and benefits far outweigh the potential iodine-induced risks. Moreover, iodine supplementation during pregnancy in orde...
journal_title：International journal of endocrinology
pub_type： 杂志文章,评审
doi：10.1155/2015/312305
更新日期：2015-01-01 00:00:00
abstract：:Objective. In pediatric central diabetes insipidus (CDI), etiology diagnosis and pituitary function monitoring are usually delayed. This study aimed to illustrate the importance of regular follow-up and pituitary function monitoring in pediatric CDI. Methods. The clinical, hormonal, and neuroradiological characteristi...
journal_title：International journal of endocrinology
pub_type： 杂志文章
doi：10.1155/2016/6365830
更新日期：2016-01-01 00:00:00
