Abstract:
:The RV144 HIV-1 vaccine trial (Thailand, 2003 to 2009), using immunogens genetically matched to the regional epidemic, demonstrated the first evidence of efficacy for an HIV-1 vaccine. Here we studied the molecular evolution of the HIV-1 epidemic from the time of immunogen selection to the execution of the efficacy trial. We studied HIV-1 genetic diversity among 390 volunteers who were deferred from enrollment in RV144 due to preexisting HIV-1 infection using a multiregion hybridization assay, full-genome sequencing, and phylogenetic analyses. The subtype distribution was 91.7% CRF01_AE, 3.5% subtype B, 4.3% B/CRF01_AE recombinants, and 0.5% dual infections. CRF01_AE strains were 31% more diverse than the ones from the 1990s Thai epidemic. Sixty-nine percent of subtype B strains clustered with the cosmopolitan Western B strains. Ninety-three percent of B/CRF01_AE recombinants were unique; recombination breakpoint analysis showed that these strains were highly embedded within the larger network that integrates recombinants from East/Southeast Asia. Compared to Thai sequences from the early 1990s, the distance to the RV144 immunogens increased 52% to 68% for CRF01_AE Env immunogens and 12% to 29% for subtype B immunogens. Forty-three percent to 48% of CRF01_AE sequences differed from the sequence of the vaccine insert in Env variable region 2 positions 169 and 181, which were implicated in vaccine sieve effects in RV144. In conclusion, compared to the molecular picture at the early stages of vaccine development, our results show an overall increase in the genetic complexity of viruses in the Thai epidemic and in the distance to vaccine immunogens, which should be considered at the time of the analysis of the trial results.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kijak GH,Tovanabutra S,Rerks-Ngarm S,Nitayaphan S,Eamsila C,Kunasol P,Khamboonruang C,Thongcharoen P,Namwat C,Premsri N,Benenson M,Morgan P,Bose M,Sanders-Buell E,Paris R,Robb ML,Birx DL,De Souza MS,McCutchan FE,Micdoi
10.1128/JVI.03070-12subject
Has Abstractpub_date
2013-07-01 00:00:00pages
7265-81issue
13eissn
0022-538Xissn
1098-5514pii
JVI.03070-12journal_volume
87pub_type
杂志文章abstract::The Epstein-Barr virus (EBV) lytic activator genes bzlf1 and brlf1 are conventionally referred to as immediate-early (IE) genes. However, previous studies showed that the earliest expression of these genes was blocked by cycloheximide when the EBV lytic cycle was induced by histone deacetylase (HDAC) inhibitors and pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01415-10
更新日期:2010-12-01 00:00:00
abstract::Although several virologic and immunologic factors associated with an increased risk of perinatal human immunodeficiency virus type 1 (HIV-1) transmission have been described, the mechanism of mother-to-child transmission is still unclear. More specifically, the question of whether selective pressures influence the tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3050-3057.2003
更新日期:2003-03-01 00:00:00
abstract::The parechoviruses differ in many biological properties from other picornaviruses, and their replication strategy is largely unknown. In order to identify the viral RNA replication complex in human parechovirus type 1 (HPEV-1)-infected cells, we located viral protein and RNA in correlation to virus-induced membrane al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8512-8523.2003
更新日期:2003-08-01 00:00:00
abstract::Analysis of the complete sequence of a human immunodeficiency virus (HIV) isolate (Ant70) obtained from a Cameroonian patient indicates that this virus is the most divergent strain within the HIV-1 family hitherto described. Comparison of the Pol protein, usually highly conserved within the HIV-1 family, shows only ab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1586-1596.1994
更新日期:1994-03-01 00:00:00
abstract::Human immunodeficiency virus type 2 (HIV-2), like other lentiviruses, is capable of infecting nondividing T cells and macrophages. The present work shows that in HIV-2-infected cells, Vpx is necessary for efficient nuclear import of the preintegration complex. In agreement with this finding, the subcellular localizati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.6162-6167.2000
更新日期:2000-07-01 00:00:00
abstract::Due to increasing concerns about human infection by various H7 influenza viruses, including recent H7N9 viruses, we evaluated the genetic relationships and cross-protective efficacies of three different Eurasian H7 avian influenza viruses. Phylogenic and molecular analyses revealed that recent Eurasian H7 viruses can ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02259-16
更新日期:2017-05-12 00:00:00
abstract::Two hundred forty pyridazinamine derivatives were tested for the ability to stabilize the antigenicity and infectivity of oral poliovirus vaccine subjected to 45 degrees C for 2 h. Seven compounds stabilized the antigenicity of all three vaccine strains and neutralized the viral particles in a way that is reversible b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3397-3400.1994
更新日期:1994-05-01 00:00:00
abstract::Purified Semliki Forest virus (SFV) contains three structural proteins while its core (nucleocapsid) contains two of these proteins. To identify all of the proteins synthesized under virus direction, cells were infected with SFV in the presence of actinomycin D and guanidine. Cell protein synthesis was markedly and ir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.10.1076-1080.1968
更新日期:1968-10-01 00:00:00
abstract::The organization of adeno-associated virus serotype 4 (AAV 4) DNA was probed by using restriction enzymes. The cleavage sites of the following enzymes were mapped: BglII, BamI, HincII, KpnI, PstI, SalI, SstI, and XhoI. The orientation of transcription on the physical cleavage map was determined by locating the fragmen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.653-661.1980
更新日期:1980-09-01 00:00:00
abstract::The rate-limiting steps in infection by human immunodeficiency virus type 1 (HIV-1) deficient in the viral infectivity factor, Vif, are unknown. As a measurement of completion of the early stages of the HIV-1 life cycle, the levels of viral DNA were examined by polymerase chain reaction amplification during infection ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.6322-6326.1993
更新日期:1993-10-01 00:00:00
abstract::The unique short (Us) region of the pseudorabies virus (PRV) genome, which displays high transcriptional activity during the late phase of infection and has been found to code for glycoproteins, is partially deleted in the genomes of three vaccine strains (A57, Norden, and NIA-4). This deletion is located in the SalI ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.307-311.1985
更新日期:1985-10-01 00:00:00
abstract::Monoclonal antibodies (MAbs) directed against epitopes in the V2 domain of human immunodeficiency virus type 1 gp120 often possess neutralizing activity, but these generally are highly type specific, neutralize only laboratory isolates, or have low potency. The most potent of these is C108g, directed against a type-sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.6909-6917.2005
更新日期:2005-06-01 00:00:00
abstract:UNLABELLED:Human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 encode auxiliary proteins that play important roles in viral replication, viral latency, and immune escape. The presence of auxiliary protein-encoding open reading frames (ORFs) in HTLV-3, the latest HTLV to be discovered, is unknown. Simian T-cell l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02150-14
更新日期:2015-01-15 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.6973-6978.1993
更新日期:1993-12-01 00:00:00
abstract::By using T1 oligonucleotide fingerprinting and mapping techniques, we analyzed the genomic structure of retroviruses produced by thymocytes and splenocytes of reciprocal bone marrow-and thymus-grafted chimeras. We found that the genetic factor(s) derived from NZB mice that suppresses the development of thymic leukemia...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.1.33-45.1983
更新日期:1983-07-01 00:00:00
abstract::In 2002, severe acute respiratory syndrome (SARS)-coronavirus (CoV) appeared as a novel human virus with high similarity to bat coronaviruses. However, while SARS-CoV uses the human angiotensin-converting enzyme 2 (ACE2) receptor for cellular entry, no coronavirus isolated from bats appears to use ACE2. Here we show t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00311-12
更新日期:2012-06-01 00:00:00
abstract::Recently, novel arenaviruses were found in snakes with boid inclusion body disease (BIBD); these form the new genus Reptarenavirus within the family Arenaviridae. We used next-generation sequencing and de novo sequence assembly to investigate reptarenavirus isolates from our previous study. Four of the six isolates an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01112-15
更新日期:2015-08-01 00:00:00
abstract::Two chimpanzees immunized with woodchuck hepatitis virus (WHV) surface antigen (WHsAg) developed antibodies cross-reactive with hepatitis B virus (HBV) surface antigen (HBsAg). After challenge with HBV, one animal was completely protected and the other experienced a subclinical infection, without evidence of liver dis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.895-901.1986
更新日期:1986-12-01 00:00:00
abstract:UNLABELLED:Plasma microRNAs (miRNAs) change in abundance in response to disease and have been associated with liver fibrosis severity in chronic hepatitis C virus (HCV) infection. However, the early dynamics of miRNA release during acute HCV infection are poorly understood. In addition, circulating miRNA signatures hav...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2015-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.17.8054-8062.2001
更新日期:2001-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) uses a variety of chemokine receptors as coreceptors for virus entry, and the ability of the virus to be neutralized by antibody may depend on which coreceptors are used. In particular, laboratory-adapted variants of the virus that use CXCR4 as a coreceptor are highly sensit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1886-1893.1998
更新日期:1998-03-01 00:00:00
abstract::To study the role of the biochemical and physiological activities of simian virus 40 (SV40) large T antigen in the lytic and transformation processes, we have analyzed DNA replication-defective, transformation-competent T-antigen mutants. Here we describe two such mutants, C8/SV40 and T22/SV40, and also summarize the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.1.120-127.1985
更新日期:1985-01-01 00:00:00
abstract::Long-term ovine fibroblast cultures contain replicative-competent lentiviruses in a latent form. This in vitro phenomenon, never described previously for lentiviruses, was clearly demonstrated by activating the expression of latent viruses with various inducing cell treatments, some of which were efficient in inducing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.680-682.1984
更新日期:1984-11-01 00:00:00
abstract::The monoclonal antibody PAb280 binds to small t antigen but not to large T antigen. Its binding site within the unique region of small t antigen was localized by studying its reaction with simian virus 40 mutants, other papovaviruses, and bacterial expression vectors coding for fragments of small t antigen. The antibo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3128-3134.1989
更新日期:1989-07-01 00:00:00
abstract::Cells of Mus minutoides, an African pygmy mouse of the subgenus Nannomys, are susceptible to ecotropic Moloney and Friend mouse leukemia viruses (MLVs) but not to AKV-type MLVs. Transfected MA139 ferret cells expressing the mCAT-1 cell surface receptor, with the minCAT-1 substitutions K222Q and V233L, did not restrict...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00202-08
更新日期:2008-07-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) DNA and RNA have been detected in peripheral nervous system (PNS) and central nervous system (CNS) tissues of latently infected mice. However, explant methods are successful in reactivating HSV-1 only from latently infected PNS tissues. In this report, latent herpesvirus infections ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.749-756.1988
更新日期:1988-03-01 00:00:00
abstract::Revertants of Kirsten sarcoma virus transformed nonproducer BALB/3T3 cells (KA31 cells) were isolated after exposing the transformed cells to 5-fluorodeoxyuridine at high cell density, or when suspended in methylcellulose. Revertants were also isolated by treating KA31 cells with the lectin, concanavalin A, which is m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.2.239-248.1974
更新日期:1974-08-01 00:00:00
abstract::In this paper, we report that the DNA of bovine mammillitis virus (BMV) consists of two covalently linked components that are 71.5 x 10(6) and 15.7 x 10(6) in molecular weight and designated L and S, respectively. We further report that the BMV DNA consists of four equimolar populations differing only in the orientati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.395-407.1978
更新日期:1978-01-01 00:00:00
abstract::We used 18 monoclonal antibodies against B19 parvovirus to identify neutralizing epitopes on the viral capsid. Of the 18 antibodies, 9 had in vitro neutralizing activity in a bone marrow colony culture assay. The overlapping polypeptide fragments spanning the B19 structural proteins were produced in a pMAL-c Escherich...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.7056-7060.1991
更新日期:1991-12-01 00:00:00
abstract::We established a system for propagation of Sindbis virus (SIN)-based replicons in tissue culture in the form of a tricomponent genome virus. Three RNA fragments containing complementing genetic information required for virus replication are packaged into separate viral particles, and each cell produces at least 1,000 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.637-643.2005
更新日期:2005-01-01 00:00:00