Abstract:
BACKGROUND:Surface charge and oxidative stress are often hypothesized to be important factors in cytotoxicity of nanoparticles. However, the role of these factors is not well understood. Hence, the aim of this study was to systematically investigate the role of surface charge, oxidative stress and possible involvement of mitochondria in the production of intracellular reactive oxygen species (ROS) upon exposure of rat macrophage NR8383 cells to silicon nanoparticles. For this aim highly monodisperse (size 1.6 ± 0.2 nm) and well-characterized Si core nanoparticles (Si NP) were used with a surface charge that depends on the specific covalently bound organic monolayers: positively charged Si NP-NH2, neutral Si NP-N3 and negatively charged Si NP-COOH. RESULTS:Positively charged Si NP-NH2 proved to be more cytotoxic in terms of reducing mitochondrial metabolic activity and effects on phagocytosis than neutral Si NP-N3, while negatively charged Si NP-COOH showed very little or no cytotoxicity. Si NP-NH2 produced the highest level of intracellular ROS, followed by Si NP-N3 and Si NP-COOH; the latter did not induce any intracellular ROS production. A similar trend in ROS production was observed in incubations with an isolated mitochondrial fraction from rat liver tissue in the presence of Si NP. Finally, vitamin E and vitamin C induced protection against the cytotoxicity of the Si NP-NH2 and Si NP-N3, corroborating the role of oxidative stress in the mechanism underlying the cytotoxicity of these Si NP. CONCLUSION:Surface charge of Si-core nanoparticles plays an important role in determining their cytotoxicity. Production of intracellular ROS, with probable involvement of mitochondria, is an important mechanism for this cytotoxicity.
journal_name
Part Fibre Toxicoljournal_title
Particle and fibre toxicologyauthors
Bhattacharjee S,de Haan LH,Evers NM,Jiang X,Marcelis AT,Zuilhof H,Rietjens IM,Alink GMdoi
10.1186/1743-8977-7-25subject
Has Abstractpub_date
2010-09-11 00:00:00pages
25issn
1743-8977pii
1743-8977-7-25journal_volume
7pub_type
杂志文章abstract:BACKGROUND:Carbon load in airway macrophages (AM) has been proposed as an internal marker to assess long-term exposure to combustion-derived pollutant particles. However, it is not known how this biomarker is affected by changes in exposure. We studied the clearance kinetics of black carbon (BC) in AM, obtained by sput...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章,评审
doi:10.1186/s12989-016-0123-y
更新日期:2016-02-29 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
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更新日期:2012-11-09 00:00:00
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journal_title:Particle and fibre toxicology
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doi:10.1186/1743-8977-7-42
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journal_title:Particle and fibre toxicology
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journal_title:Particle and fibre toxicology
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
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journal_title:Particle and fibre toxicology
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doi:10.1186/s12989-016-0175-z
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-018-0279-8
更新日期:2018-11-13 00:00:00
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doi:10.1186/s12989-018-0285-x
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-24
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-14
更新日期:2013-04-15 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-5-19
更新日期:2008-12-04 00:00:00
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journal_title:Particle and fibre toxicology
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-3
更新日期:2013-02-12 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-019-0318-0
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-9-24
更新日期:2012-07-06 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章,评审
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-38
更新日期:2013-08-09 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-7
更新日期:2009-03-13 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章,评审
doi:10.1186/s12989-017-0237-x
更新日期:2018-01-03 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-017-0213-5
更新日期:2017-08-30 00:00:00
abstract:BACKGROUND:Silica nanoparticles (nanoSiO2) are promising systems that can deliver biologically active compounds to tissues such as the heart in a controllable manner. However, cardiac toxicity induced by nanoSiO2 has been recently related to abnormal calcium handling and energetic failure in cardiomyocytes. Moreover, t...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-020-00346-2
更新日期:2020-05-07 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-33
更新日期:2013-07-30 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-48
更新日期:2013-10-04 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 评论,信件
doi:10.1186/s12989-020-00365-z
更新日期:2020-07-16 00:00:00
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journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-016-0178-9
更新日期:2016-12-12 00:00:00