Abstract:
BACKGROUND:Carbon nanotubes (CNT) can interact with the biological environment, which could participate in their associated toxicity. We recently demonstrated that pH is an important player of CNT fate inside macrophages. We wanted to further characterize such process, and therefore designed a study dedicated to decipher CNT biodegradation by macrophages, as a function of two major physico-chemical properties in regard with nanotoxicology; length and degree of functionalization. To achieve our aim, we synthesized, following a single initial production process, four MWCNT differing in length and/or surface chemistry: S-CNT (short), SF-CNT (short functionalized), L-CNT (long) and LF-CNT (long functionalized). RESULTS:Raman spectroscopy analysis performed on CNT recovered after exposure of RAW 264.7 macrophages for 6, 24, or 48 h demonstrate that CNT show early signs of biodegradation over time inside macrophages. The modulation of CNT length and functionalization, resulting in the modification of iron accessibility, both represent critical determinants of the biodegradation process; short pristine CNT were more prone to biodegradation than long CNT (pristine or functionalized), while short functionalized CNT were protected. Incubation of cells with Concanamycin completely prevents CNT from being modified, demonstrating that this biodegradation process is dependent on an intracellular pH-dependent mechanism. Interestingly, and despite evidence of degradation via Raman spectroscopy, the CNT length and diameter were not altered during the course of the study. CONCLUSIONS:In conclusion, our results identify a new mechanism of CNT biodegradation inside macrophages. This could give new insights for the understanding of CNT-associated toxicity, and represent important tools to develop safe(r)-by-design nanomaterials.
journal_name
Part Fibre Toxicoljournal_title
Particle and fibre toxicologyauthors
Landry M,Pinault M,Tchankouo S,Charon É,Ridoux A,Boczkowski J,Mayne-L'Hermite M,Lanone Sdoi
10.1186/s12989-016-0175-zsubject
Has Abstractpub_date
2016-11-24 00:00:00pages
61issue
1issn
1743-8977pii
10.1186/s12989-016-0175-zjournal_volume
13pub_type
杂志文章abstract:BACKGROUND:The unique physicochemical properties of multi-walled carbon nanotubes (MWCNT) have led to many industrial applications. Due to their low density and small size, MWCNT are easily aerosolized in the workplace making respiratory exposures likely in workers. The International Agency for Research on Cancer desig...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-019-0318-0
更新日期:2019-10-07 00:00:00
abstract::There are certain concerns regarding the safety for the environment and human health from the use of engineered nanoparticles (ENPs) which leads to unintended exposures, as opposed to the use of ENPs for medical purposes. This review focuses on the unintended human exposure of ENPs. In particular, possible effects in ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章,评审
doi:10.1186/1743-8977-7-42
更新日期:2010-12-21 00:00:00
abstract:BACKGROUND:Using engineered nanomaterial-based toners, laser printers generate aerosols with alarming levels of nanoparticles that bear high bioactivity and potential health risks. Yet, the cardiac impacts of printer-emitted particles (PEPs) are unknown. Inhalation of particulate matter (PM) promotes cardiovascular mor...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-019-0335-z
更新日期:2020-01-29 00:00:00
abstract:BACKGROUND:The complement system is a key component of innate immunity implicated in the neutralization and clearance of invading pathogens. Dextran coated superparamagnetic iron oxide (SPIO) nanoparticle is a promising magnetic resonance imaging (MRI) contrast agent. However, dextran SPIO has been associated with sign...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-014-0064-2
更新日期:2014-11-26 00:00:00
abstract::Air pollution is a growing public health concern of global significance. Acute and chronic exposure is known to impair cardiovascular function, exacerbate disease and increase cardiovascular mortality. Several plausible biological mechanisms have been proposed for these associations, however, at present, the pathways ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章,评审
doi:10.1186/s12989-017-0237-x
更新日期:2018-01-03 00:00:00
abstract:BACKGROUND:Even though a continuously high number of in vitro studies on nanoparticles are being published, the issue of correct dose matter is often not sufficiently taken into account. Due to their size, the diffusion of nanoparticles is slower, as compared to soluble chemicals, and they sediment slowly. Therefore, t...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-018-0278-9
更新日期:2018-10-30 00:00:00
abstract:BACKGROUND:Photocopiers emit nanoparticles with complex chemical composition. Short-term exposures to modest nanoparticle concentrations triggered upper airway inflammation and oxidative stress in healthy human volunteers in a recent study. To further understand the toxicological properties of copier-emitted nanopartic...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-42
更新日期:2013-08-22 00:00:00
abstract::This review considers the molecular toxicology of combustion-derived nanoparticles (CDNP) following inhalation exposure. CDNP originate from a number of sources and in this review we consider diesel soot, welding fume, carbon black and coal fly ash. A substantial literature demonstrates that these pose a hazard to the...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-2-10
更新日期:2005-10-21 00:00:00
abstract::In their Commentary Saber et al. (Part Fibre Toxicol 16: 44, 2019) argue that chronic inhalation studies in rats can be used for assessing the lung cancer risk of insoluble nanomaterials. The authors make several significant errors in their interpretation and representation of the underlying science. In this Letter to...
journal_title:Particle and fibre toxicology
pub_type: 评论,信件
doi:10.1186/s12989-020-00365-z
更新日期:2020-07-16 00:00:00
abstract:BACKGROUND:Mitochondria are both a sensitive target and a primary source of oxidative stress, a key pathway of air particulate matter (PM)-associated diseases. Mitochondrial DNA copy number (MtDNAcn) is a marker of mitochondrial damage and malfunctioning. We evaluated whether ambient PM exposure affects MtDNAcn in a hi...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-17
更新日期:2013-04-29 00:00:00
abstract:BACKGROUND:Nanometer silicon dioxide (nano-SiO2) has a wide variety of applications in material sciences, engineering and medicine; however, the potential cell biological and proteomic effects of nano-SiO2 exposure and the toxic mechanisms remain far from clear. RESULTS:Here, we evaluated the effects of amorphous nano...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-7-1
更新日期:2010-01-19 00:00:00
abstract:BACKGROUND:Nano- or microscale copper oxide particles (CuO NP, CuO MP) are increasingly applied as catalysts or antimicrobial additives. This increases the risk of adverse health effects, since copper ions are cytotoxic under overload conditions. METHODS:The extra- and intracellular bioavailability of CuO NP and CuO M...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-11-10
更新日期:2014-02-13 00:00:00
abstract:BACKGROUND:Carbon nanotubes and nanofibers (CNT/F) have known toxicity but simultaneous comparative studies of the broad material class, especially those with a larger diameter, with computational analyses linking toxicity to their fundamental material characteristics was lacking. It was unclear if all CNT/F confer sim...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-020-00392-w
更新日期:2020-12-07 00:00:00
abstract:BACKGROUND:The Metropolitan Area of São Paulo has a unique composition of atmospheric pollutants, and positive correlations between exposure and the risk of diseases and mortality have been observed. Here we assessed the effects of ambient fine particulate matter (PM2.5) on genotoxic and global DNA methylation and hydr...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-018-0276-y
更新日期:2018-10-19 00:00:00
abstract:BACKGROUND:Inhalative nanocarriers for local or systemic therapy are promising. Gold nanoparticles (AuNP) have been widely considered as candidate material. Knowledge about their interaction with the lungs is required, foremost their uptake by surface macrophages and epithelial cells. METHODS:Scnn1b-Tg and Wt mice inh...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-19
更新日期:2013-05-16 00:00:00
abstract:BACKGROUND:Surface charge and oxidative stress are often hypothesized to be important factors in cytotoxicity of nanoparticles. However, the role of these factors is not well understood. Hence, the aim of this study was to systematically investigate the role of surface charge, oxidative stress and possible involvement ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-7-25
更新日期:2010-09-11 00:00:00
abstract:BACKGROUND:The etiology and progression of neurodegenerative disorders depends on the interactions between a variety of factors including: aging, environmental exposures, and genetic susceptibility factors. Enhancement of proinflammatory events appears to be a common link in different neurological impairments, includin...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-7-12
更新日期:2010-05-17 00:00:00
abstract:BACKGROUND:Particulate air pollution is associated with increased risk of cardiovascular disease and stroke. Although the precise mechanisms underlying this association are still unclear, the induction of systemic inflammation following particle inhalation represents a plausible mechanistic pathway. METHODS:We used ba...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-9-24
更新日期:2012-07-06 00:00:00
abstract:BACKGROUND:Carbon load in airway macrophages (AM) has been proposed as an internal marker to assess long-term exposure to combustion-derived pollutant particles. However, it is not known how this biomarker is affected by changes in exposure. We studied the clearance kinetics of black carbon (BC) in AM, obtained by sput...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-018-0250-8
更新日期:2018-03-14 00:00:00
abstract:BACKGROUND:In myeloid cells the inflammasome plays a crucial role in innate immune defenses against pathogen- and danger-associated patterns such as crystalline silica. Respirable mineral particles impinge upon the lung epithelium causing irreversible damage, sustained inflammation and silicosis. In this study we inves...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-3
更新日期:2013-02-12 00:00:00
abstract:BACKGROUND:Exposure to small size particulate matter in urban air is regarded as a risk factor for cardiovascular effects, whereas there is little information about the impact on the cardiovascular system by exposure to pure carbonaceous materials in the nano-size range. C60 fullerenes are nano-sized particles that are...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-5
更新日期:2009-02-25 00:00:00
abstract:BACKGROUND:Epidemiological studies suggest that prenatal and early life environmental exposures have adverse effects on pulmonary function and are important contributors in the development of childhood asthma and allergic disease. The mechanism by which environmental tobacco smoke (ETS) exposure in utero promotes the d...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-017-0212-6
更新日期:2017-08-22 00:00:00
abstract:BACKGROUND:Engineered carbon nanotubes are currently used in many consumer and industrial products such as paints, sunscreens, cosmetics, toiletries, electronic processes and industrial lubricants. Carbon nanotubes are among the more widely used nanoparticles and come in two major commercial forms, single-walled carbon...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-11-3
更新日期:2014-01-09 00:00:00
abstract:BACKGROUND:Inhaled nanoparticles have been reported in some instances to translocate from the nostril to the olfactory bulb in exposed rats. In close proximity to the olfactory bulb is the olfactory mucosa, within which resides a niche of multipotent cells. Cells isolated from this area may provide a relevant in vitro ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-10-54
更新日期:2013-10-21 00:00:00
abstract:BACKGROUND:In nanotoxicology, the exact role of particle shape, in relation to the composition, on the capacity to induce toxicity is largely unknown. We investigated the toxic and immunotoxic effects of silver wires (length: 1.5 - 25 μm; diameter 100 - 160 nm), spherical silver nanoparticles (30 nm) and silver micropa...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-8-36
更新日期:2011-12-30 00:00:00
abstract:OBJECTIVE:Gold nanoparticles have attracted enormous interest as potential theranostic agents. However, little is known about the long-term elimination and systemic toxicity of gold nanoparticles in the literature. Hollow gold nanospheres (HAuNS) is a class of photothermal conducting agent that have shown promises in p...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-11-26
更新日期:2014-05-30 00:00:00
abstract:BACKGROUND:Epidemiologic and animal studies have shown that particulate air pollution is associated with increased risk of lung and cardiovascular diseases. Although the exact mechanisms by which particles induce cardiovascular diseases are not known, studies suggest involvement of systemic acute phase responses, inclu...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/1743-8977-6-12
更新日期:2009-04-20 00:00:00
abstract:BACKGROUND:Inhalation of environmental (nano) particles (NP) as well as persistent herpesvirus-infection are potentially associated with chronic lung disease and as both are omnipresent in human society a coincidence of these two factors is highly likely. We hypothesized that NP-exposure of persistently herpesvirus-inf...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-016-0181-1
更新日期:2017-01-10 00:00:00
abstract:BACKGROUND:Fine particulate matter (PM2.5) related mild inflammation, altered autonomic control of cardiovascular function, and changes to cell function have been observed in controlled human exposure studies. METHODS:To measure the systemic and cardiopulmonary impacts of low-level PM exposure, we exposed 20 healthy, ...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-020-00389-5
更新日期:2020-11-16 00:00:00
abstract:BACKGROUND:Increasing evidence from toxicological and epidemiological studies indicates that the central nervous system is an important target for ambient air pollutants. We have investigated whether long-term inhalation exposure to diesel engine exhaust (DEE), a dominant contributor to particulate air pollution in urb...
journal_title:Particle and fibre toxicology
pub_type: 杂志文章
doi:10.1186/s12989-017-0213-5
更新日期:2017-08-30 00:00:00