Abstract：
:Rice ragged stunt virus (RRSV) is a very important virus that infects rice and causes serious yield losses in Asian countries and other major rice planting areas. Thus, it is urgent to establish an efficient and practical approach for identification and diagnosis in the field. Our results indicated that reverse transcription loop-mediated isothermal amplification (RT-LAMP) reactions are more efficient and sensitive than RT-PCR for RRSV detection. The optimal LAMP conditions were as follows: 0.4-1.2 μM internal primers, 0.2-0.25 μM external primers, 0.8 μM loop primers, and incubation at 62 °C or 63 °C for 30 min. Furthermore, the RT-LAMP primers specifically targeted RRSV virus and resulted in typical waterfall-like bands by gel electrophoresis and sigmoidal amplification curves. The primers could not be used to amplify other common plant viruses including Papaya ringspot virus (PRSV), Rice yellow stunt virus (RYSV), Sorghum mosaic virus (SrMV), Cactus virus X (CVX), Melon yellow spot virus (MYSV) and Southern rice black-streaked dwarf virus (SRBSDV). Ten-fold serial dilutions of RRSV cDNA indicated that RT-LAMP is much faster and at least ten times more sensitive than RT-PCR in detecting the virus. The waterfall-like product bands could be observed within one hour. In the field study, about 77% samples were identified as RRSV. RT-LAMP has many benefits over RT-PCR such as low cost and high accuracy, sensitivity, and specificity. This technology meets the requirements for rapid diagnosis of plant virus diseases in the field to best guide management practices for growers.
journal_name
Saudi J Biol Scijournal_title
Saudi journal of biological sciencesauthors
Lai D,Zhang Y,Huang Q,Yin G,Pennerman KK,Liu Z,Guo Adoi
10.1016/j.sjbs.2016.02.024subject
Has Abstractpub_date
2018-12-01 00:00:00pages
1577-1584issue
8eissn
1319-562Xissn
2213-7106pii
S1319-562X(16)00082-6journal_volume
25pub_type
杂志文章abstract：:Influenza viruses are recognized as one of the major causes of respiratory tract infection in young children and elderly people throughout the world. In this report, influenza B virus was detected when 200 clinical samples of nasopharyngeal aspirates, collected from hospitalized children aged from one month to three y...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2009.10.008
更新日期：2009-10-01 00:00:00
abstract：Objectives:Comparison of Ticagrelor vs clopidogrel in antiplatelet therapeutic effect of acute myocardial infarction patients undergoing percutaneous coronary intervention. Methods:The study focused on 2000 acute myocardial infarction patients undergoing percutaneous coronary intervention (PCI) in our hospital from Ja...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.11.020
更新日期：2017-12-01 00:00:00
abstract：:This study was carried out to evaluate the anti-obesity effect of Vernonia amygdalina Del. (VA) supplemented diet. VA leaf powder was fed at 5% and 15% to diet-induced obese rats for 4 weeks and its effect compared with orlistat (5.14 mg/kg p.o.), an anti-obesity drug. Food intake, body and organ weights, total body f...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2012.05.003
更新日期：2012-07-01 00:00:00
abstract：:A new and simple protocol has been developed and standardized for direct somatic embryogenesis and plant regeneration from aseptic seedlings derived from immature Brassica juncea seeds. Depending on the age of immature seeds and nutrient media, in vitro occurrence of embryogenesis and the number of embryos from each s...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.11.050
更新日期：2021-01-01 00:00:00
abstract：:Sex differences are present in all parts of the body, including the skeletal system. Several methods are used to analyze the sex differences of skeleton, while more recently, a new method called geometric morphometry has been used. The aim of this study was to examine the sexual dimorphism of occipital condyles on hum...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.09.019
更新日期：2020-12-01 00:00:00
abstract：:Feeding ecology of any species can help us to understand its natural history, ecological requirements and approaches involved in searching for food. Feeding ecology and sexual dimorphism in the horned viper, Cerastes cerastes gasperetti collected from the Al-Thumama area, central region of Saudi Arabia was described. ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.10.010
更新日期：2016-01-01 00:00:00
abstract：:Asian citrus psyllid is a most damaging insect pest of citrus. In this field study, the efficacy of seven insecticides (emamectin benzoate, bifenthrin, chlorfenapyr, fipronil, imidacloprid, pyriproxyfen and thiamethoxam) was evaluated against Diaphorina citri Kuwayama in the citrus orchard of Kinnow mandarin, Citrus r...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.02.006
更新日期：2020-05-01 00:00:00
abstract：:This research aimed to study the diversity of purple nonsulfur bacteria (PNSB) and to investigate the effect of Hg concentrations in shrimp ponds on PNSB diversity. Amplification of the pufM gene was detected in 13 and 10 samples of water and sediment collected from 16 shrimp ponds in Southern Thailand. In addition to...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.05.014
更新日期：2016-07-01 00:00:00
abstract：:Artemisinin is the first-line drugs for the treatment of malaria. In recent years, a large number of reports showed that artemisinin exhibit anti-tumor activity. In this study, we used C6 glioma cells and rat C6 brain-glioma model to study anti-tumor activity of artemisinin in vivo and in vitro. We found that artemisi...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.04.003
更新日期：2018-11-01 00:00:00
abstract：Background:The antimicrobial resistance of extraintestinal pathogenic Escherichia coli (ExPEC) has progressively been reported worldwide. This resistance has been ascribed to global dissemination of a single E. coli clone, namely E. coli sequence type 131 (E. coli ST131). The main goal of this study is to determine the...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.09.020
更新日期：2020-01-01 00:00:00
abstract：:In forensic science, the human identification is the major goal in criminal cases and in paternity dispute, based on DNA profiling of individual. Analysis of short tandem repeat (STRs) markers used as a reliable technique for this purpose. In this study the main objective was to develop a human identification based on...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.10.001
更新日期：2018-12-01 00:00:00
abstract：:This study aims to present an integrated process that can be used to produce biomedical and biological active components from the fruit shell of Camellia oleifera Abel. Through the Foss method, Aldehyde, acid compounds, acyl and alcohol compounds account for 22.7, 15.93, 0.24 and 61.13% of the extractives which were e...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.08.006
更新日期：2018-02-01 00:00:00
abstract：:The present study aims to explore the crisis of antibiotic resistance and discover more about the current challenges related to self-medication. The current challenges related to antibiotic resistance are unique and differ from the challenges of the past since new bacterial pathogens are involved and continue to evolv...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2017.01.004
更新日期：2017-05-01 00:00:00
abstract：:Regular exposure to stress causes alteration in biochemical parameter but till date no specific medicine prescribed for controlling it. Current study aimed to determine the effect of Diazepam on proinflammatory and cardiac markers in stress exposed rats. Male Wistar rats were divided into four groups with six animals ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.10.042
更新日期：2021-01-01 00:00:00
abstract：:Partial root-zone drying during irrigation (PRD) has been shown effective in enhancing plant water use efficiency (WUE), however, the roles of chemical signals from root and shoot that are involved and the possible interactions affected by nitrogen nutrition are not clear. Pot-grown cotton (Gossypium spp.) seedlings w...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.01.015
更新日期：2017-03-01 00:00:00
abstract：:Foodborne pathogens are one of the major cause of food-related diseases and food poisoning. Bacterial biofilms and quorum sensing (QS) mechanism of cell-cell communication have also been found to be associated with several outbreaks of foodborne diseases and are great threat to food safety. Therefore, In the present s...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2018.04.013
更新日期：2019-02-01 00:00:00
abstract：:Tuberculosis (TB) is a disease that affects one-third of the world's population. Although currently available TB drugs have many side effects, such as nausea, headache and gastrointestinal discomfort, no new anti-TB drugs have been produced in the past 30 years. Therefore, the discovery of a new anti-TB agent with min...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2020.07.007
更新日期：2020-09-01 00:00:00
abstract：:The traditional medicine based on medicinal plants in the Kingdom of Arabia Saudia presents a strong relationship belonging to natural remedies, health, diet, and folk healing practice recognized by a specific culture. The aim of the current study is to carry out an ethnobotanical review on medicinal plants used in tr...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2020.06.020
更新日期：2020-10-01 00:00:00
abstract：:A halotolerant actinobacterial strain isolated from salinity affected soil of Eastern Indo-Gangetic plains (IGP), Uttar Pradesh, India, was characterised for its antagonistic potential against Macrophomina phaseolina by dual-culture assay. It was shown to effectively inhibit the growth of M. phaseolina with an inhibit...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.12.004
更新日期：2017-01-01 00:00:00
abstract：:Methicillin-Resistant Staphylococcus aureus (MRSA) represents one of the major causes of nosocomial infections, leading to high mortality. Surfaces in clinics, as well as the attending uniform and the hands of the dental doctor can be MRSA reservoirs. Having this in mind, the purpose of this study was to evaluate the ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2019.12.003
更新日期：2020-04-01 00:00:00
abstract：:Food poisoning caused by Staphylococcus aureus (S. aureus) toxins is considered one of the foremost public health threat that usually occurs through the ingestion of raw milk contaminated with staphylococcal enterotoxins. The current study spotlights on the prevalence, antibiogram and genetic diversity of S. aureus en...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.06.028
更新日期：2020-08-01 00:00:00
abstract：:Food borne diseases are a major public health concern worldwide. Staphylococcus aureus is one of the potential food borne pathogens which causes nosocomial and community acquired infections. In the present study, 74 representative strains of S. aureus isolated and characterized in previous study from different milk sa...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.10.011
更新日期：2018-11-01 00:00:00
abstract：:Legumes are essential and play a significant role in maintaining food standards and augmenting physiochemical soil properties through the biological nitrogen fixation process. Biotic and abiotic factors are the main factors limiting legume production. Classical breeding methodologies have been explored extensively abo...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章,评审
doi：10.1016/j.sjbs.2019.11.018
更新日期：2020-01-01 00:00:00
abstract：:Antibiotic resistance is a problem of deep scientific concern both in hospital and community settings. Rapid detection in clinical laboratories is essential for the judicious recognition of antimicrobial resistant organisms. Production of extended-spectrum β-lactamases (ESBLs) is a significant resistance-mechanism tha...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2014.08.002
更新日期：2015-01-01 00:00:00
abstract：:The effects of exposing meat-type breeder eggs to magnetic field (MF) before incubation on hatchability traits (percents of hatchability and hatchability failures of eggs), chick weight at hatch, and post-hatch performance (weight gain, feed intake, and feed conversion ratio (FCR)) from 1 to 39 d of age were investiga...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2011.06.004
更新日期：2011-10-01 00:00:00
abstract：:Over the last decade, communication industries have witnessed a tremendous expansion, while, the biological effects of electromagnetic waves have not been fully elucidated. Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2015.05.001
更新日期：2016-05-01 00:00:00
abstract：:Accurate and up to date land use and land cover (LU/LC) changes information is the main source to understanding and assessing the environmental outcomes of such changes and is important for development plans. Thus, this study quantified the outlines of land cover variation of 10-years in the northwestern costal land o...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2020.07.021
更新日期：2020-11-01 00:00:00
abstract：AIM:The dietary contents have a very important role in the management of metabolic syndrome along with type 2 diabetes mellitus (T2DM). Indian diet contains a large amount of carbohydrates that set off unpredictable blood sugar fluctuations and leads to increased risk of diabetic complications. The aim of the present s...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2014.04.005
更新日期：2015-01-01 00:00:00
abstract：:Conservation in the Kingdom of Saudi Arabia is relatively young, yet have made considerable gains in conservation through strategic proclamation and reintroductions. Changes in land use, illegal hunting and competition with domestic stock has decimated the native ungulates, meaning that the survival of the native ungu...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.03.009
更新日期：2018-02-01 00:00:00
abstract：:Bamboo macromolecules were pretreated with bamboo vinegar, which has an antibacterial property, and processed into antibacterial bioboard (ABB) by hot pressing. The ABB was then analyzed by conducting Fourier-transform infrared spectroscopy, thermogravimetric analysis and differential thermal analysis. Results showed ...
journal_title：Saudi journal of biological sciences
pub_type： 杂志文章
doi：10.1016/j.sjbs.2017.08.010
更新日期：2018-03-01 00:00:00
