Effect of long-term exposure to mobile phone radiation on alpha-Int1 gene sequence of Candida albicans.

Abstract:

:Over the last decade, communication industries have witnessed a tremendous expansion, while, the biological effects of electromagnetic waves have not been fully elucidated. Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans. A standard 900 MHz radiation generator was used for radiation. 10 ml volumes from a stock suspension of C. albicans were transferred into 10 polystyrene tubes. Five tubes were exposed at 4 °C to a fixed magnitude of radiation with different time periods of 10, 70, 210, 350 and 490 h. The other 5 tubes were kept far enough from radiation. The samples underwent genomic DNA extraction. PCR amplification of alpha-Int1 gene sequence was done using one set of primers. PCR products were resolved using agarose gel electrophoresis and the nucleotide sequences were determined. All samples showed a clear electrophoretic band around 441 bp and further sequencing revealed the amplified DNA segments are related to alpha-Int1 gene of the yeast. No mutations in the gene were seen in radiation exposed samples. Long-term exposure of the yeast to mobile phone radiation under the above mentioned conditions had no mutagenic effect on alpha-Int1 gene sequence.

journal_name

Saudi J Biol Sci

authors

Shahin-Jafari A,Bayat M,Shahhosseiny MH,Tajik P,Roudbar-Mohammadi S

doi

10.1016/j.sjbs.2015.05.001

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

426-33

issue

3

eissn

1319-562X

issn

2213-7106

pii

S1319-562X(15)00102-3

journal_volume

23

pub_type

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