Abstract:
:Key pathways like insulin signaling, AMP activated kinase (AMPK) activation and inflammatory signaling are involved in the complex pathological network of hepatic insulin resistance. Our aim is to investigate whether grape seed proanthocyanidins (GSP) and metformin (MET) target any of these pathways in insulin resistant rat liver. Albino Wistar rats were rendered insulin resistant by feeding a high fat-fructose diet (HFFD). Either GSP (100 mg/kg b.w), MET(50 mg/kg b.w) or both were administered to insulin resistant rats as therapeutic options. HFFD-feeding caused hyperglycemia, hyperinsulinemia, increased gluconeogenesis, decreased tyrosine phosphorylation of insulin receptor-β(IR-β) and insulin receptor substrate-1 (IRS-1) and increased serine phosphorylation of IRS-1. The association of p85α subunit of phosphotidyl inositol 3 kinase(PI3K) with IRS-1 and subsequent Akt phosphorylation were reduced while the expression of mitogen activated protein kinases (MAPK) were increased in HFFD rats. Both MET and GSP reduced hyperglycemia and hyperinsulinemia and improved glycolysis, tyrosine phosphorylation of IR-β and IRS-1, IRS-1-PI3K association and Akt activation. However, activation of tumor necrosis factor-α, interleukin-6, leptin and suppressor of cytokine signaling-3 and reduction in adiponectin caused by chronic HFFD feeding were reversed by GSP better than by MET. Activation of AMPK by GSP was much less compared to that by MET. These findings suggest that GSP might activate PI3K pathway and promote insulin action by reducing serine kinase activation and cytokine signaling and MET by targeting AMPK. The beneficial effects were enhanced during combination therapy. Thus, combination therapy with MET and GSP may be considered for the management of metabolic syndrome.
journal_name
J Cell Commun Signaljournal_title
Journal of cell communication and signalingauthors
Yogalakshmi B,Bhuvaneswari S,Sreeja S,Anuradha CVdoi
10.1007/s12079-013-0210-xsubject
Has Abstractpub_date
2014-03-01 00:00:00pages
13-22issue
1eissn
1873-9601issn
1873-961Xjournal_volume
8pub_type
杂志文章abstract::The lectin from Canavalia ensiformis (Concanavalin-A, ConA), one of the most abundant lectins known, enables one to mimic biological lectin/carbohydrate interactions that regulate extracellular matrix protein recognition. As such, ConA is known to induce membrane type-1 matrix metalloproteinase (MT1-MMP) which express...
journal_title:Journal of cell communication and signaling
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doi:10.1007/s12079-009-0084-0
更新日期:2010-03-01 00:00:00
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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doi:10.1007/s12079-016-0362-6
更新日期:2017-03-01 00:00:00
abstract::We recently reported that transgenic mice with cardiac-restricted overexpression of CCN2/CTGF have substantially increased tolerance towards ischemia/reperfusion injury. The purpose of this study was to investigate to what extent fully differentiated cardiac myocytes are direct targets of CCN2, and to resolve the sign...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-012-0183-1
更新日期:2013-03-01 00:00:00
abstract::Periostin is a secretory protein with a multi-domain structure, comprising an amino-terminal cysteine-rich EMI domain, four internal FAS 1 domains, and a carboxyl-terminal hydrophilic domain. These adjacent domains bind to extracellular matrix proteins (type I collagen, fibronectin, tenascin-C, and laminin γ2), and BM...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章,评审
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更新日期:2018-03-01 00:00:00
abstract::CCN3 is a member of the CCN family of cell growth and differentiation regulators that play key roles during embryonic development, and are associated with severe human pathologies. The level of CCN genes' expression is of prognostic value in several types of tumors. In the present manuscript, we report the isolation a...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-007-0009-8
更新日期:2007-09-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-012-0176-0
更新日期:2012-12-01 00:00:00
abstract::Mice lacking the pro-adhesive matricellular protein connective tissue growth factor (CTGF/CCN2) display an embryonic lethal phenotype due to defects in bone and cartilage. However, the specific role of CCN2 in skin development is unknown. Here, we generated mice deleted for CCN2 in the entire body (using a cre/lox sys...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-011-0129-z
更新日期:2011-08-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-008-0031-5
更新日期:2008-12-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-015-0261-2
更新日期:2015-06-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-020-00546-7
更新日期:2020-12-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-015-0281-y
更新日期:2015-03-01 00:00:00
abstract::The CCN family of proteins consists of six members with conserved structural features. These proteins play several roles in the physiology and pathology of cells. Among the pathological roles of the CCN family, one of the most important and controversial ones is their role in the expansion and metastasis of cancer. Up...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-011-0133-3
更新日期:2011-12-01 00:00:00
abstract::Fibroblast growth factor 1 (FGF-1) is the first FGF family member, and it induces proliferation of fibroblasts and other types of the cells. However, recent studies are uncovering unexpected functions of this molecule. Our previous study redefined this growth factor as a catabolic molecule produced in cartilage upon m...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-020-00600-4
更新日期:2021-01-04 00:00:00
abstract::Extracellular molecules coordinate the multiple signaling pathways spatiotemporally to exchange information between cells during development. Understanding the regulation of these signal molecule-dependent pathways elucidates the mechanism of intercellular crosstalks. CCN2/CTGF is one of the CCN family members that bi...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-018-0487-x
更新日期:2019-03-01 00:00:00
abstract::Connexin43 (Cx43) gap junctions expressed in astrocytes can significantly impact neuronal survival in stroke. However, little is known regarding Cx43 spatial and temporal expression during the initial stages of brain ischemia. Using immunohistochemistry and Western blot analysis, we examined Cx43 spatial and temporal ...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-017-0430-6
更新日期:2018-03-01 00:00:00
abstract::In June 2015, Thomson Reuters informed our publisher Springer that the Journal of Cell Communication and Signaling, the official journal of the International CCN Society, « had been selected for coverage in Thomson Reuter's products and services. Beginning with V. 1 (1) 2007, this publication would be indexed and abst...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-015-0306-6
更新日期:2015-09-01 00:00:00
abstract::Lung immaturity is the major cause of morbidity and mortality in premature infants, especially those born <28 weeks of gestation. These infants are at high risk of developing respiratory distress syndrome (RDS), a lung disease caused by insufficient surfactant production and immaturity of saccular/alveolar type II epi...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-017-0443-1
更新日期:2018-03-01 00:00:00
abstract::UNC-5 Homolog B (UNC5B) is a member of the dependence receptor family. This family of receptors can induce two opposite intracellular signaling cascades depending on the presence or absence of the ligand and is thus capable of driving two opposing processes. UNC5B signaling has been implicated in several cancers, wher...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-018-0485-z
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abstract::Research over the past few years has highlighted the ability of the unfolded protein response (UPR) to minimize the deleterious effects of accumulated misfolded proteins under both physiological and pathological conditions. The endoplasmic reticulum (ER) adapts to endogenous and exogenous stressors by expanding its pr...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-014-0251-9
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-008-0034-2
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-017-0396-4
更新日期:2017-09-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-017-0432-4
更新日期:2018-03-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-017-0439-x
更新日期:2018-09-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章,评审
doi:10.1007/s12079-016-0321-2
更新日期:2016-06-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-018-00504-4
更新日期:2019-09-01 00:00:00
abstract::The Fifth International Workshop on the CCN Family of Genes was held in Toronto October 18-22, 2008. This bi-annual workshop provides a unique opportunity for the presentation and discussion of cutting edge research in the CCN field. The CCN family members have emerged as extracellular matrix associated proteins which...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-009-0036-8
更新日期:2008-12-01 00:00:00
abstract::The discovery of the gap junction structure, its functions and the family of the "connexin" genes, has been basically ignored by the major biological disciplines. These connexin genes code for proteins that organize to form membrane-associated hemi-channels, "connexons", co-join with the connexons of neighboring cells...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-010-0108-9
更新日期:2011-03-01 00:00:00
abstract::Mitogen activated protein kinases (MAPKs) play an important role in activation, differentiation and proliferation of macrophages. Macrophages, upon activation, produce large amounts of nitric oxide that inhibit the growth of variety of microorganisms and tumor cells. This nitric oxide which is known to interfere with ...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-008-0021-7
更新日期:2007-12-01 00:00:00
abstract::There is no therapy for chronic fibroproliferative diseases, in spite of the fact that current health statistics suggest that these (which include cardiovascular disease, pulmonary fibrosis, diabetic nephropathy, liver cirrhosis and systemic sclerosis) have been estimated to cause approximately 45% of the deaths in th...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-011-0156-9
更新日期:2012-03-01 00:00:00