Abstract:
:The Rsu1 protein contributes to cell adhesion and migration via its association with the adaptor complex of Integrin linked kinase (ILK), PINCH, and Parvin (IPP), which binds to the cytoplasmic domain of β1 integrins joining integrins to the actin cytoskeleton. Rsu1 binding to PINCH in the IPP complex is required for EGF-induced adhesion, spreading and migration in MCF10A mammary epithelial cells. In addition, Rsu1 expression inhibits Jun kinase but is necessary for the activation of MKK4 and p38 Map kinase signaling essential for migration in MCF10A cells. The data reported here examines the links between MKK4-p38-ATF2 signaling and AKT regulation in MCF10A cells. Ectopic Rsu1 inhibited AKT1 phosphorylation while Rsu1 depletion induced AKT activation and AKT1 phosphorylation of MKK4 on serine 80, blocking MKK4 activity. Rsu1 depletion also reduced the RNA for lipid phosphatase PTEN thus implicating PTEN in modulating levels of activated AKT in these conditions. ChIP analysis of the PTEN promoter revealed that Rsu1 depletion prevented binding of ATF2 to a positive regulatory site in the PTEN promoter and the enhanced binding of cJun to a negatively regulatory PTEN promoter site. These results demonstrate a mechanism by which Rsu1 adhesion signaling alters the balance between MKK4-p38-ATF2 and cJun activation thus altering PTEN expression in MCF10A cells.
journal_name
J Cell Commun Signaljournal_title
Journal of cell communication and signalingauthors
Kim YC,Gonzalez-Nieves R,Cutler MLdoi
10.1007/s12079-018-00504-4subject
Has Abstractpub_date
2019-09-01 00:00:00pages
331-341issue
3eissn
1873-9601issn
1873-961Xpii
10.1007/s12079-018-00504-4journal_volume
13pub_type
杂志文章abstract::The ubiquitin proteasome pathway plays a critical role in regulating many processes in the cell which are important for tumour cell growth and survival. Inhibition of proteasome function has emerged as a powerful strategy for anti-cancer therapy. Clinical validation of the proteasome as a therapeutic target was achiev...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-011-0121-7
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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更新日期:2007-12-01 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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doi:10.1007/s12079-008-0021-7
更新日期:2007-12-01 00:00:00
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abstract::Glutamine (gln) metabolism has emerged as a cancer therapeutic target in past few years, however, the effect of gln-deprivation of bCSCs remains elusive in breast cancer. In this study, effect of glutamine on stemness and differentiation potential of bCSCs isolated from MCF-7 and MDAMB-231 were studied. We have shown ...
journal_title:Journal of cell communication and signaling
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更新日期:2021-01-28 00:00:00
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-017-0428-0
更新日期:2018-03-01 00:00:00
abstract::Hepatocyte exosomes (ExoHep) are proposed to mediate physiological or pathophysiological signaling in a variety of hepatic target cells. ExoHep were purified from the medium of primary mouse hepatocytes or AML12 cells and characterized as ~100 nm nanovesicles that were positive for proteins commonly found in exosomes ...
journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-017-0421-7
更新日期:2018-03-01 00:00:00
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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更新日期:2015-09-01 00:00:00
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journal_title:Journal of cell communication and signaling
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pub_type: 杂志文章,评审
doi:10.1007/s12079-018-0492-0
更新日期:2019-09-01 00:00:00
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journal_title:Journal of cell communication and signaling
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doi:10.1007/s12079-017-0439-x
更新日期:2018-09-01 00:00:00
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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更新日期:2018-03-01 00:00:00
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journal_title:Journal of cell communication and signaling
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doi:10.1007/s12079-015-0281-y
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
doi:10.1007/s12079-020-00600-4
更新日期:2021-01-04 00:00:00
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
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journal_title:Journal of cell communication and signaling
pub_type: 杂志文章
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更新日期:2020-10-13 00:00:00
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