Abstract:
:G protein-coupled receptors (GPCRs) require G proteins for intracellular signaling to regulate a variety of growth and maintenance processes, including osteogenesis and bone turnover. Bone maintenance events may be altered by changes in the activity or level of G proteins, which then modify signaling in bone cells such as osteoblasts. We have previously reported increased levels of Gα11 protein and signaling to phospholipase C/protein kinase C pathways in response to dexamethasone in osteoblastic UMR 106-01 cells. Here we generated pOBCol3.6-GNA11 transgenic mice that overexpress Gα11 protein in cells of the osteoblast lineage (G11-Tg mice). G11-Tg mice exhibit an osteopenic phenotype characterized by significant reductions in trabecular bone mineral density, thickness, number and strength. The numbers of osteoblasts and osteocytes were unchanged in G11-Tg bone, but early markers of osteoblast differentiation, Alp and Bsp, were increased while the late stage differentiation marker Ocn was not changed suggesting reduced osteoblast maturation in G11-Tg trabecular bone which was accompanied by a decreased bone formation rate. Furthermore, in vitro cultures of G11-Tg primary osteoblasts show delayed osteoblast differentiation and mineralization. Histological analyses also revealed increased osteoclast parameters, accompanied by elevated mRNA expression of Trap and Ctsk. mRNA levels of Rankl and M-csf were elevated in vitro in bone marrow stromal cells undergoing osteogenesis and in trabecular bone in vivo. Together, these findings demonstrate that increasing Gα11 protein expression in osteoblasts can alter gene expression and result in a dual mechanism of trabecular bone loss.
journal_name
Bonejournal_title
Boneauthors
Dela Cruz A,Mattocks M,Sugamori KS,Grynpas MD,Mitchell Jdoi
10.1016/j.bone.2013.11.021subject
Has Abstractpub_date
2014-02-01 00:00:00pages
211-22eissn
8756-3282issn
1873-2763pii
S8756-3282(13)00483-3journal_volume
59pub_type
杂志文章相关文献
BONE文献大全abstract::This in vivo study investigated the influence of two calcium phosphate particle sizes (40-80 microm and 200-500 microm) on the cellular degradation activity associated with the bone substitution process of two injectable bone substitutes (IBS). The tested biomaterials were obtained by associating a biphasic calcium ph...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00137-4
更新日期:1999-08-01 00:00:00
abstract:BACKGROUND:NF-κB essential modulator (NEMO), encoded by IKBKG, is necessary for activation of the ubiquitous transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Animal studies suggest NEMO is required for NF-κB mediated bone homeostasis, but this has not been thoroughly studied ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.01.014
更新日期:2019-04-01 00:00:00
abstract::Levels of circulating cytokines are elevated in inflammatory diseases. Previously, it was shown that interleukin (IL-)17A, in synergism with 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and tumor necrosis factor α (TNFα), induces the release of granulocyte-macrophage colony-stimulating factor (GM-CSF) by murine osteoblasts ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.01.036
更新日期:2017-04-01 00:00:00
abstract::Pre-clinical in vivo models of multiple myeloma are essential tools for investigating the pathophysiology of multiple myeloma and for testing new therapeutic agents and strategies prior to their potential use in clinical trials. Over the last five decades, several different types of murine models of multiple myeloma h...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2015.04.004
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:To estimate the cost-effectiveness of strontium ranelate in the treatment of postmenopausal osteoporotic women aged over 75 years. MATERIALS AND METHODS:A validated Markov microsimulation model with a Belgian payer's perspective estimated the cost per quality-adjusted life-year (QALY) of a 3-year strontium r...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.08.052
更新日期:2010-02-01 00:00:00
abstract::Bone metastases and skeletal complications are major causes of morbidity in prostate cancer patients. Despite the osteoblastic appearance of bone metastases on imaging studies, patients have elevated serum and urinary markers of bone resorption, indicative of high osteoclast activity. Increased osteoclast activity is ...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2010.05.038
更新日期:2011-01-01 00:00:00
abstract::The metabolic fate of whole grafts that were either vascularized or nonvascularized were compared. This study was designed to quantify and correlate changes in bone resorption, formation, and mass in orthotopic, stably fixed, weight-bearing autografts. The grafts were 8-cm segments of the fibula that were internally f...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(91)90018-e
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Epimedium-derived flavonoids (EFs) have a potential to treat established osteoporosis in postmenopausal women. However, one of the main disadvantages of the compound is the high volume and dosage during long-term administration period. Meanwhile, the beneficial effect of EFs on osteoporotic bone depends grea...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.02.012
更新日期:2013-07-01 00:00:00
abstract::Ginsenoside Rh2 is one of the most active components of red ginseng, controlling cancer and other metabolic diseases including osteoclast differentiation. However, the molecular mechanism underlying the inhibition of osteoclast differentiation by ginsenoside Rh2 remains poorly understood. In the present study, it was ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.03.022
更新日期:2012-06-01 00:00:00
abstract::Growing evidence suggests that positive associations between fat mass (FM) and bone mineral density (BMD) are mediated by not only biomechanical but also biochemical factors. Adiponectin is a novel adipocyte-derived hormone that regulates energy homeostasis and has anti-inflammatory and anti-atherogenic effects. Unlik...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00237-0
更新日期:2003-10-01 00:00:00
abstract::In order to evaluate the effects of uremia and low levels of dietary phosphorus on bone, male Sprague-Dawley rats weighing 320 +/- 20 g (12 weeks old) were subjected to either a two-step, subtotal nephrectomy or sham-operation (SO), and then fed a custom diet with either normal calcium (0.5%) and normal phosphorus (0....
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(90)90070-f
更新日期:1990-01-01 00:00:00
abstract::Regulation of calcium release from mouse calvariae by glycosaminoglycans and known modulators of bone resorption has been examined. Anticoagulant and nonanticoagulant fragments of heparin were combined with parathyroid hormone (PTH) and interleukin-1, beta (IL1), and calcium release from radiolabeled bone was determin...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(88)90018-x
更新日期:1988-01-01 00:00:00
abstract::Trabecular bone fracture represents a major health problem, therefore the improvement of its assessment is mandatory for the reduction of the economic and social burden. The micro-structure of the trabecular bone was found to have an important effect on trabecular mechanical behavior. Nonetheless, the high variability...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.12.007
更新日期:2014-03-01 00:00:00
abstract:UNLABELLED:We identified regions inside the proximal femur that are most strongly associated with hip fracture. Bone densitometry based on such fracture-critical regions showed improved power in discriminating fracture patients from controls. INTRODUCTION:Hip fractures typically occur in lateral falls, with focal mech...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.12.008
更新日期:2009-04-01 00:00:00
abstract::Cartilage canals are temporary vessel-containing structures within the growth cartilage. The canals gradually regress with age in a process designated chondrification, where the content of the canals is replaced by cartilage. The process of chondrification is considered physiological; however, premature regression has...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.08.016
更新日期:2004-12-01 00:00:00
abstract::Endochondral ossification in the growth plate of long bones involves cartilage mineralization, bone formation and the budding vasculature. Many of these processes take place in a complex and dynamic zone, the provisional ossification zone, of the growth plate. Here we investigate aspects of mineralization in 2D and 3D...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115086
更新日期:2020-01-01 00:00:00
abstract::Intracortical remodeling in the adult skeleton removes and replaces areas of compact bone that have sustained microdamage. Although studies have been performed in animal species in which there is an existing baseline of remodeling activity, laboratory rodents have been considered to have limited suitability as models ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00104-5
更新日期:1998-09-01 00:00:00
abstract::Bisphosphonates are widely used in the treatment of osteoporosis and contribute to the reduction of bone fractures. Ibandronate (IBN) is a highly potent, nitrogen-containing bisphosphonate, which is administered orally or intravenously at extended dosing intervals. Vitamin D or active vitamin D3 derivatives are also u...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.08.004
更新日期:2015-12-01 00:00:00
abstract::The relative bioavailability of enteric-coated sodium fluoride (NaF) tablets (10 mg F-) has been assessed following administration with a standard calcium-rich breakfast or calcium-poor lunch, and 2 h before or simultaneously with antacid administration (2.4 g aluminum-magnesium hydroxide), versus intake on an empty s...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(89)90070-7
更新日期:1989-01-01 00:00:00
abstract::Treatment with parathyroid hormone 1-84 (PTH) or teriparatide increases osteonal remodeling and decreases bone mineral density (BMD) at cortical (Ct) bone sites but may also increase bone size. Decreases in BMD and increases in size exert opposing effects on bone strength. In adult ovariectomized (OVX) rhesus monkeys,...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.04.197
更新日期:2007-09-01 00:00:00
abstract::This cohort study of postmenopausal women in the United Kingdom aged ≥50years determined the incremental cost of health care and clinical outcomes in the 12months following incident, selected fractures (non-vertebral non-hip [NVNHF], vertebral [VF] and multiple [MF]). Incremental costs and outcomes of the fracture coh...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.05.020
更新日期:2012-09-01 00:00:00
abstract::The uncarboxylated form of the osteoblast-specific secreted molecule osteocalcin is a hormone favoring glucose handling and increasing energy expenditure. As a result, the absence of osteocalcin leads to glucose intolerance in mice, while genetically modified mice with an increase in uncarboxylated osteocalcin are pro...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.04.017
更新日期:2012-02-01 00:00:00
abstract::The degradation of the main fibrillar collagens, collagens I and II, is a crucial process for skeletal development. The most abundant dipeptides generated from the catabolism of collagens contain proline and hydroxyproline. In humans, prolidase is the only enzyme able to hydrolyze dipeptides containing these amino aci...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.11.009
更新日期:2015-03-01 00:00:00
abstract::To better understand the effects of prolonged bed-rest in women, 24 healthy women aged 25 to 40 years participated in 60-days of strict 6° head-down tilt bed-rest (WISE-2005). Subjects were assigned to either a control group (CON, n=8) which performed no countermeasure, an exercise group (EXE, n=8) undertaking a combi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.06.021
更新日期:2011-10-01 00:00:00
abstract::The Rickets Severity Score (RSS) was used to evaluate X-linked hypophosphatemic rickets (XLH), a genetic disorder mediated by increased circulating FGF23. The reliability of the RSS was assessed using data from a randomized, phase 2 clinical trial that evaluated the effects of burosumab, a fully human anti-FGF23 monoc...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.02.010
更新日期:2019-05-01 00:00:00
abstract::We investigated the function of Ca2+ in the activation of phosphatidylcholine (PC)-hydrolyzing phospholipase D (PLD) in osteoblast-like MC3T3-E1 cells. Fetal calf serum (FCS) stimulated the formation of choline in a dose-dependent manner in the range between 0.6% and 10%. The effect of a combination of FCS and 12-O-te...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(96)00185-8
更新日期:1996-10-01 00:00:00
abstract::Subject motion during high-resolution peripheral quantitative computed tomography (HR-pQCT) causes image artifacts that affect morphological analysis of bone quality. The aim of our study was to determine effectiveness of techniques for quality control in the presence of motion in vivo including automated and manual a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.03.003
更新日期:2012-06-01 00:00:00
abstract::Although basic fibroblast growth factor (bFGF) is a potent stimulator of bone formation when administered intravenously, less is known regarding the effects of this peptide on bone following subcutaneous (s.c.) administration. In addition, it is unknown whether coadministration of estrogen enhances the bone response t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00118-2
更新日期:2003-09-01 00:00:00
abstract:AIMS:Hip fracture patients have severe deterioration of their quality of life and function after their injury. Markers of malnutrition such as low albumin and low body mass index (BMI) have been shown to increase mortality and complication rates but their effect on recovery of quality of life and function after hip fra...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115567
更新日期:2021-02-01 00:00:00
abstract::Disuse osteopenia and bone loss have been extensively reported in long duration space mission and long term bed rest. The pathology of the bone loss is similar to osteoporosis but highly confined to weight bearing bones. The current anabolic and/or anti-resorptive drugs have systemic effects and are costly over extend...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.01.020
更新日期:2015-06-01 00:00:00