Abstract：
BACKGROUND/AIM:There is no clear evidence in the literature regarding the regulation of programmed cell death-ligand 1 (PD-L1) expression by cyclo-oxygenase-2 (COX2). In this study, whether PD-L1 expression was regulated by COX2 activity was examined in vitro. MATERIALS AND METHODS:Resected lung cancer specimens were analyzed for PD-L1 and COX2 expression by immunohistochemical analysis. Next, co-localization of PD-L1 and COX2 expression was analyzed by double-fluorescence staining. Lastly, the effect of COX2 inhibition on the expression of PD-L1 was examined using lung cancer cell lines. RESULTS:PD-L1 expression was significantly correlated with COX2 expression in the resected specimens. The majority of cancer cells that expressed PD-L1 also co-expressed COX2. However, treatment of lung cancer cell lines with a COX2 inhibitor had no impact on PD-L1 expression. CONCLUSION:Our results suggest that COX2 inhibition might have no effect on the usage of immune checkpoint inhibitors in lung cancer treatment.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Shimizu K,Okita R,Saisho S,Maeda AI,Nojima Y,Nakata Mdoi
10.21873/anticanres.12768subject
Has Abstractpub_date
2018-08-01 00:00:00pages
4637-4644issue
8eissn
0250-7005issn
1791-7530pii
38/8/4637journal_volume
38pub_type
杂志文章abstract：BACKGROUND:Dihydropyrimidine dehydrogenase (DPD) is the rate-limiting enzyme of 5-fluorouracil (5-FU) catabolism. Several studies have demonstrated the clinical importance of DPD in cancer patients, suggesting that the efficacy of 5-FU may be related to DPD activity in tumor tissue. In the present study, DPD activity a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:Basal level of asparagine synthetase mRNA in BALB3T3 cells was elevated when the cells were shifted from medium containing a high concentration (3.3 mM) of asparagine to one lacking asparagine. We then studied whether the expression of asparagine synthetase mRNA is also mediated through other asparagine-independent si...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:An important variable in determining in vitro cell survival is the time interval between treatment and assay. One manifestation of radiation damage is the introduction of a division delay in which the post-radiation division rate is less than the pre-radiation division rate. We have examined the influence of length of...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：:Serotoninergic system activity and cytokine production have been both implicated in the pathogenesis of cancer anorexia. To verify the existence of relationships between tryptophan, cytokines and anorexia, twenty cancer and six non-neoplastic patients were studied. Plasma amino acid concentration, including tryptophan...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：BACKGROUND/AIM:Neuroblastoma is a pediatric solid tumor refractory to eradication by chemotherapy. To determine whether Aloe vera (AV), a pontial anticancer reagent, could be useful in neuroblastoma therapy, we investigated the anti-proliferative effects of an AV protein extract. MATERIALS AND METHODS:Human neuroblast...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-08-01 00:00:00
abstract：BACKGROUND:Incidence of brain metastases (BM) from breast cancer (BC) is increasing. However, prognostic evaluation and treatment strategies are still a matter of debate. AIM:To describe the clinical outcome of BM from BC treated by neurosurgical resection and to identify the actual prognostic factors in this specific...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
abstract：AIMS AND BACKGROUND:Radiation therapy holds a fundamental role in oncological emergencies such as superior vena cava syndrome, spinal cord compression and endocranial hypertension. The purpose of our study was, by comparing schedules of treatment, to confirm the efficacy of hypofractionated radiation therapy. METHODS:...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:We studied a consecutive series of 54 cases of lower lip squamous cell carcinoma (LLSCC) in order to identify any variables which might predict the development of lymph node metastases. The cases were divided into 38 tumors without metastases (group I) and 16 tumors with lymph node metastases (group II). The following...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:In a prospective study comprising 447 women with endometrial carcinoma stages I-II, the prognostic significance of clinical and flow cytometric variables was evaluated in univariate and multivariate analyses. The parameters studied included age, uterine cavity depth, clinical stage, histopathologic grade, myometrial i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a widespread environmental contaminant that induces various types of immunotoxicity. One effect of exposure to this contaminant is alteration in cell adhesiveness. Leukocyte function-associated antigen-1 (LFA-1) plays an important role not only in T-cell recruitment into s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：BACKGROUND/AIM:The main objective of the present study was to evaluate the significance of apoptosis in the Fletcher's risk classification for gastrointestinal stromal tumors (GISTs). MATERIALS AND METHODS:Apoptotic cells were identified by immunostaining for single-stranded DNA (ssDNA). We assigned each GIST to one o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12542
更新日期：2018-05-01 00:00:00
abstract：AIM:To elucidate the behavior of 1 phosphatidylinositol 4,5 bisphosphate (PIP2) 5 phosphatase (PIP2 5-Pase, EC 3.1.3.36) and 1 phosphatidylinositol 4 phosphate (PIP) 4-phosphatase (PIP 4-Pase) in cancer cells, the steady state activities of PIP2 5-Pase and PIP 4-Pase were determined in the particulate fractions of rat ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:While reserpine and nicardipine pretreatment induced a more or less marked decrease in typical red-orange doxorubicin fluorescence in certain rat tissues, such as spleen, kidney and large intestine, the myocardial tissue (right atrium and left ventriculum) did not show any significant variations in nuclear doxorubicin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:The karyotypes and selected isoenzymes of five cell lines, HU 961b, HU 1922, HU 609, HCV-29 and HU 1703 He, were characterized. These cell lines were derived either from histologically normal human urothelial cells or from transitional cell carcinoma (TCC) of the urinary bladder. They displayed different life span in ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:We investigated the signal transduction pathway to low-dose radiation-induced apoptosis in vitro in the human peripheral primitive neuroectodermal tumor (pPNET) cell line with wild-type p53 established in our laboratory. Apoptosis was induced by 2Gy irradiation in an almost p53-dependent manner in this model except fo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：BACKGROUND:Immunotherapy has become an attractive new strategy in the treatment of cancer. The laboratory and clinical study of cancer immunotherapy is rapidly advancing. However, in the clinical setting, the results of cancer immunotherapy are mixed. We therefore contend that cancer immunotherapy should be customized ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：:The androgen-signaling pathway plays a critical role in prostate cancer development and progression. We have recently demonstrated that the Wilms' tumor suppressor gene product, WT1, binds to multiple sites in the androgen receptor (AR) promoter and transcriptionally represses the AR gene promoter in vitro. We asked w...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND/AIM:Fluorescence-guided surgery (FGS) of cancer is an emerging technology. We have previously shown the importance of resecting both the tumor and the tumor microenvironment (TME) for curative FGS. We also previously developed a syngeneic model using the mouse lymphoma cell line EL-4, expressing red fluoresc...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.10988
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND:Malignant transformation in endometriosis is a rare but well known complication. However, detailed mechanisms of malignant transition and tumourigenesis in endometriosis remain unknown. We here describe the case of an endometrioid carcinoma arisen in endometriotic ovarian cyst fulfilling Sampson's criteria f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:During follow-up examinations for breast cancer the serum tumor marker CA 125 was measured routinely in the Universitätsfrauenklinik Erlangen. We found elevated CA 125 levels in 18 of 510 clinical asymptomatic patients. These patients were followed closely. A second malignant neoplasm was found in six of these patient...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Growth inhibitory activity of YM881 (SMANCS) against 3 human cultured cell lines and one rat cultured cell line derived from glioma cells was assessed quantitatively. YM881 showed potent cytotoxicity against all glioma cell lines tested; the IC50 of this drug was 1.9-8.4 micrograms/ml. YM881 may be classified as a con...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：BACKGROUND:Breast cancer grows in a hormone-rich environment which influences its biological features and thus, ultimately, its clinical behavior. Circulating hormone levels were measured in premenopausal and postmenopausal breast cancer patients prior to surgery, and correlated with all available clinical and biologic...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Laryngeal hyperkeratotic lesions can progress to fully developed malignant carcinoma in some cases. These premalignant lesions are proliferative disorders whose potential for further tumour progression is perhaps difficult to assess by mere histology. Immunostaining with PCNA, a protein correlated with cell proliferat...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:5-Fluorouracil (5FU) increases the rate of incorporation of tritiated thymidine (3HTdR) and iododeoxyuridine (125 IUdR) into the cell and its DNA. 5FU has differential effect on metabolism of DNA through its specific action on thymidylate synthetase. Effects of 5FU on the incorporation of other metabolic precursors we...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1982-05-01 00:00:00
abstract：BACKGROUND:Ewing's sarcoma cell lines may represent a good in vitro model for the understanding of tumor biology in this heterogeneous group of diseases. In the present study, we report the establishment and characterization of a primary Ewing's sarcoma cell line (LDS-Falck 01). MATERIALS AND METHODS:LDS-Falck 01 was ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:We report two cases with small cell lung cancer (SCLC) receiving multicyclic dose-intensified chemotherapy (DI-CT) supported by autologous blood progenitor cell transplantation (ABPCT). Both cases were initially treated with cisplatin (CDDP), etoposide (ETP) and concurrent thoracic irradiation, however they had recurr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND/AIM:There is no definite consensus regarding management of hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT). This study aimed to compare clinical outcomes according to initial treatment modality for treatment of naïve HCC combined with subsegmental (Vp1)/ segmental (Vp2) PVTT with live...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12808
更新日期：2018-08-01 00:00:00
abstract：AIM:The aim of the present study was to evaluate how treatment with everolimus changes readouts of the pulmonary function test (PFT) in patients with metastatic renal cell carcinoma (mRCC). We also attempted to determine whether changes of PFT or everolimus-associated non-infectious pneumonitis (NIP) might affect the e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND/AIM:Reportedly, hypertension tends to be associated with response to bevacizumab therapy, because bevacizumab suppresses vascular nitric oxide production. In this study we examined the predictive value of nitric oxide in bevacizumab-treated non-small cell lung cancer (NSCLC) patients. PATIENTS AND METHODS:F...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11676
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND:Tyrosine kinase inhibitors (TKIs) directed against epidermal growth factor receptor (EGFR) are important in the treatment of non-small cell lung cancer (NSCLC), especially those harboring EGFR mutations. But little is known regarding the clinical value of serum tumor marker levels measured prior to treatment...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12018
更新日期：2017-10-01 00:00:00
