Abstract：
:Calcineurin was isolated from bovine cerebrum extracts by sequential chromatography on Affi-Gel blue and calmodulin affinity columns. Calcineurin so isolated was approximately 90% pure and was composed of equimolar amounts of subunit A (Mr = 61 000-63 000) and subunit B (Mr = 15 000-17 000) when examined by sodium dodecyl sulfate gel electrophoresis. A polypeptide (less than 10%) with Mr = 71 000 whose function and role remains to be investigated, was routinely detected in the calcineurin preparation. Both inhibitory activity (towards calmodulin-dependent cAMP phosphodiesterase) and phosphatase activity (with 32P-labelled myelin basic protein as substrate) were associated with calcineurin as evidenced by (i) coelution from Affi-Gel blue, Affi-Gel calmodulin, diethythaminoethyl-Sepharose, and Sephacryl S-200 chromatography columns; (ii) association with the same protein band on nondenaturing gels; (iii) similar stability upon storage at 4 degrees C and with repeated freezing and thawing; and (iv) parallel heat inactivation. Phosphatase activity of calcineurin was maximal with 32P-labelled myelin basic protein as the substrate. Using this substrate, enzyme activity was generally stimulated 5- to 10-fold in the presence of Ca2+ and calmodulin; half-maximal activation (A0.5) was observed with 25 nM calmodulin. Calmodulin increased the Vmax of the reaction without affecting the Km for the substrate. Optimum temperature and pH for the reaction were 45 degrees C and 7, respectively, in both the absence and presence of Ca2+ and calmodulin.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Gupta RC,Khandelwal RL,Sulakhe PVdoi
10.1139/y85-165subject
Has Abstractpub_date
1985-08-01 00:00:00pages
1000-6issue
8eissn
0008-4212issn
1205-7541journal_volume
63pub_type
杂志文章abstract：:Administration of d-fenfluramine, a serotonin-releasing drug, to male rats induced a dose-dependent increase in both serum prolactin and corticosterone concentrations. Serum growth hormone levels increased, but not significantly, at a dose of 1.25 mg/kg i.p. and decreased significantly at higher doses. When rats were ...
journal_title：Canadian journal of physiology and pharmacology
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journal_title：Canadian journal of physiology and pharmacology
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journal_title：Canadian journal of physiology and pharmacology
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journal_title：Canadian journal of physiology and pharmacology
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journal_title：Canadian journal of physiology and pharmacology
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journal_title：Canadian journal of physiology and pharmacology
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更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1995-07-01 00:00:00
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更新日期：2010-02-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
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更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1139/y86-099
更新日期：1986-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-08-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y84-246
更新日期：1984-12-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
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更新日期：2020-08-15 00:00:00
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pub_type： 杂志文章
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更新日期：1986-03-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
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pub_type： 杂志文章
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更新日期：1990-11-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
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更新日期：2003-06-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y95-195
更新日期：1995-09-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
doi：10.1139/cjpp-2014-0463
更新日期：2015-10-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
doi：10.1139/y95-173
更新日期：1995-09-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y2012-097
更新日期：2012-11-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y86-215
更新日期：1986-10-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y75-073
更新日期：1975-08-01 00:00:00
