Abstract：
:Current cardiac cell therapies cannot effectively target and retain cells in a specific area of the heart. Cell-seeded biological sutures were previously developed to overcome this limitation, demonstrating targeted delivery with > 60% cell retention. In this study, both cell-seeded and non-seeded fibrin-based biological sutures were implanted into normal functioning rat hearts to determine the effects on mechanical function and fibrotic response. Human mesenchymal stem cells (hMSCs) were used based on previous work and established cardioprotective effects. Non-seeded or hMSC-seeded sutures were implanted into healthy athymic rat hearts. Before cell seeding, hMSCs were passively loaded with quantum dot nanoparticles. One week after implantation, regional stroke work index and systolic area of contraction (SAC) were evaluated on the epicardial surface above the suture. Cell delivery and retention were confirmed by quantum dot tracking, and the fibrotic tissue area was evaluated. Non-seeded biological sutures decreased SAC near the suture from 0.20 ± 0.01 measured in sham hearts to 0.08 ± 0.02, whereas hMSC-seeded biological sutures dampened the decrease in SAC (0.15 ± 0.02). Non-seeded sutures also displayed a small amount of fibrosis around the sutures (1.0 ± 0.1 mm2 ). Sutures seeded with hMSCs displayed a significant reduction in fibrosis (0.5 ± 0.1 mm2 , p < 0.001), with quantum dot-labelled hMSCs found along the suture track. These results show that the addition of hMSCs attenuates the fibrotic response observed with non-seeded sutures, leading to improved regional mechanics of the implantation region. Copyright © 2014 John Wiley & Sons, Ltd.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Tao ZW,Favreau JT,Guyette JP,Hansen KJ,Lessard J,Burford E,Pins GD,Gaudette GRdoi
10.1002/term.1904subject
Has Abstractpub_date
2017-01-01 00:00:00pages
220-230issue
1eissn
1932-6254issn
1932-7005journal_volume
11pub_type
杂志文章abstract：:The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair. A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprot...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2403
更新日期：2018-01-01 00:00:00
abstract：:The objective was to improve proliferation and chondrogenic potential of adipose stem cells (ASCs) by expansion on extracellular matrix (ECM) deposited by either ASCs or synovium-derived stem cells (SDSCs). ASCs isolated from porcine infrapatellar fat pad were separately expanded on conventional plastic flasks, ASC-de...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.505
更新日期：2013-01-01 00:00:00
abstract：:Unique poly(l-lactic acid) (PLLA)-based scaffolds were constructed by embedding knitted PLLA yarns within a bioresorbable and differentially crosslinked three-dimensional (3D) oxidized collagen scaffold. The scaffolds were designed specifically for the repair of complex incisional abdominal wall hernias and the repair...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1837
更新日期：2016-10-01 00:00:00
abstract：:Islet replacement is a promising strategy for the treatment of patients with type 1 diabetes and patients who have undergone total pancreatectomy. Recent progress in cellular reprogramming technology may allow the transplantation of a patient's own pancreatic cells. Although many studies have reported the differentiat...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2228
更新日期：2017-11-01 00:00:00
abstract：:Tissue engineering applications require an appropriate combination of a cell population, biochemical factors and scaffold materials. In this field, mesenchymal stem cells (MSCs) emerge as an attractive cell population, due to their ready availability and their potential to be differentiated into various mesodermal cel...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.545
更新日期：2013-07-01 00:00:00
abstract：:In this study human bone marrow stromal cells (hBMSCs) were cultured on three-dimensional porous scaffolds of biomimetically mineralized collagen type I developed for bone engineering. Three different cell numbers were used for seeding of the nanocomposites, and the impact of the seeding density on proliferation and o...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.110
更新日期：2008-10-01 00:00:00
abstract：:Electrospinning has recently gained widespread attention as a process capable of producing nanoscale fibres that mimic native extracellular matrix. In this study, we compared the osteogenic differentiation behaviour of human adipose stem cells (ASCs) on a 3D nanofibre matrix of type I rat tail collagen (RTC) and a 2D ...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.85
更新日期：2008-06-01 00:00:00
abstract：:Prudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bo...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3124
更新日期：2020-11-01 00:00:00
abstract：:The development of scaffolds that combine the delivery of drugs with the physical support provided by electrospun fibres holds great potential in the field of nerve regeneration. Here it is proposed the incorporation of ibuprofen, a well-known non-steroidal anti-inflammatory drug, in electrospun fibres of the statisti...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1792
更新日期：2016-03-01 00:00:00
abstract：:Bone marrow stromal cells (BMSC) transplantation enhances functional recovery after cerebral infarct, but the optimal delivery route is undetermined. This study was aimed to assess whether a novel cell-sheet technology non-invasively serves therapeutic benefits to ischemic stroke. First, the monolayered cell sheet was...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1920
更新日期：2017-02-01 00:00:00
abstract：BACKGROUND:According to experimental studies, mesh implants may reduce in size up to 50.8% during their integration into soft tissues. This results in impaired mobility of the anterior abdominal wall and hernia recurrences, as well as affects patients' quality of life. Due to unsatisfactory radiographic contrast of pol...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3029
更新日期：2020-05-01 00:00:00
abstract：:This study investigates whether platelet-rich plasma (PRP) is an activator of tendon-derived stem cells (TDSCs) to promote regeneration of Achilles tendon post-rupture in rats. In the in vitro study, PRGF (activated PRP) significantly enhanced cell DNA synthesis, improved viability and promoted proliferation, while fa...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2020
更新日期：2017-04-01 00:00:00
abstract：:Prevascularization is essential to ensure the viability, functionality, and successful integration of tissue-engineered three-dimensional (3D) constructs with surrounding host tissues after transplantation. Human mesenchymal stem cell (hMSC) sheet can be prevascularized by coculturing with endothelial cells (ECs), and...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3167
更新日期：2021-01-11 00:00:00
abstract：:This work reports the effects of phosphoserine addition on the biodegradability of calcium phosphate cements. The characteristics of a phosphoserine-modified calcium phosphate cement without collagen in a large animal model are presented here for the first time. Critical size bone defects in the proximal tibia of 10 s...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.283
更新日期：2011-01-01 00:00:00
abstract：:Rapid vascular remodelling of damaged dermal tissue is required to heal burn wounds. Thymosin β4 (Tβ4) is a growth factor that has been shown to promote angiogenesis and dermal wound repair. However, the underlying mechanisms based on Tβ4 function have not yet been fully investigated. In the present study, we investig...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2028
更新日期：2017-04-01 00:00:00
abstract：:Bone marrow (BM) multipotent mesenchymal stromal cells (MSCs) present with multipotent differentiation potential and immunomodulatory properties. As an alternative to bone marrow, we have examined fetal membranes, amnion and chorion, of term human placenta as a potential source of multipotent MSCs. Here we show that a...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.40
更新日期：2007-07-01 00:00:00
abstract：:Rapid advances in the isolation of multipotent progenitor cells, routinely called mesenchymal stromal/stem cells (MSCs), from various human tissues and organs have provided impetus to the field of cell therapy and regenerative medicine. The most widely studied sources of MSCs include bone marrow, adipose, muscle, peri...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.2914
更新日期：2019-09-01 00:00:00
abstract：:The aim of this study was to investigate the effects of a novel bioactive material (Biosilicate®) and low-level laser therapy (LLLT) on bone fracture consolidation in osteoporotic rats. Forty female Wistar rats were submitted to ovariectomy (OVX) to induce osteopenia. Eight weeks after surgery, the animals were random...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.309
更新日期：2011-03-01 00:00:00
abstract：:Transplantation of bone marrow mesenchymal stem cells (BM-MSCs) has been shown to ameliorate the injured central nervous system (CNS). Although these effects were initially attributed to the putative differentiation of MSCs towards the neural lineage, it is now known that most of them are mediated by the secretome. Up...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.365
更新日期：2011-08-01 00:00:00
abstract：:Implanted porous precision templated scaffolds (PTS) with 40-µm spherical pores reduce inflammation and foreign body reaction (FBR) while increasing vascular density upon implantation. Larger or smaller pores, however, promote chronic inflammation and FBR. While macrophage (MØ) recruitment and polarization participate...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3160
更新日期：2021-01-01 00:00:00
abstract：:Over the last few years, the generation of induced pluripotent stem cells (iPSCs) from human somatic cells has proved to be one of the most potentially useful discoveries in regenerative medicine. iPSCs are becoming an invaluable tool to study the pathology of different diseases and for drug screening. However, severa...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.2021
更新日期：2016-11-01 00:00:00
abstract：:Adipose stem cells (ASCs) represent a cell population with great potential for tissue engineering applications. Several articles have been published showing the proliferation and differentiation potential, the markers and the wide range of potential applications of these cells. In the majority of these studies the ASC...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.141
更新日期：2009-02-01 00:00:00
abstract：:Organ transplantation is an effective treatment for chronic organ dysfunctioning conditions. However, a dearth of available donor organs for transplantation leads to the death of numerous patients waiting for a suitable organ donor. The potential of decellularized scaffolds, derived from native tissues or organs in th...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.2061
更新日期：2017-04-01 00:00:00
abstract：:The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.2597
更新日期：2018-03-01 00:00:00
abstract：:Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been considered to be a promising strategy for wound healing. However, poor viability of engrafted BMSCs and limited capabilities of differentiation into the desired cell types in wounds often hinder its application. Few studies report the induction of ...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2400
更新日期：2018-02-01 00:00:00
abstract：:In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time. Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compr...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2938
更新日期：2020-01-01 00:00:00
abstract：:The effects of the stiffness of substrates on the cell behaviours of human bone marrow-derived mesenchymal stem cells (hBMSC) have been investigated, but the effects of the secondary structures of proteins in the substrates on the morphological transformation and differentiation of hBMSC have yet been elucidated. To i...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2616
更新日期：2018-04-01 00:00:00
abstract：:The mechanism for stem cell-mediated improvement following acute myocardial infarction has been actively debated. We support hypotheses that the stem cell effect is primarily paracrine factor-linked. We used a heparin-presenting injectable nanofibre network to bind and deliver paracrine factors derived from hypoxic co...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.273
更新日期：2010-12-01 00:00:00
abstract：:Self-sustainability after implantation is one of the critical obstacles facing large engineered tissues. A preformed functional vascular network provides an effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered t...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2512
更新日期：2018-03-01 00:00:00
abstract：:Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have potential clinical utility in the treatment of a multitude of ailments and diseases, due to their relative ease of isolation from patients and their capacity to form many cell types. However, hBM-MSCs are sparse, and can only be isolated in very small qu...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1515
更新日期：2014-03-01 00:00:00
