Cultivation of human bone marrow stromal cells on three-dimensional scaffolds of mineralized collagen: influence of seeding density on colonization, proliferation and osteogenic differentiation.

Abstract:

:In this study human bone marrow stromal cells (hBMSCs) were cultured on three-dimensional porous scaffolds of biomimetically mineralized collagen type I developed for bone engineering. Three different cell numbers were used for seeding of the nanocomposites, and the impact of the seeding density on proliferation and osteogenic differentiation of hBMSCs was investigated. In addition, the effect of the seeding cell number on seeding efficiency and distribution of the cells within the scaffolds was studied. Our data revealed that the open and interconnecting porosity of the mineralized collagen scaffolds allows a very efficient seeding for all seeding densities tested. Although penetration of the cells into the interior of the scaffolds was demonstrated for all seeding densities, the application of higher cell numbers resulted in a better colonization also of the deeper scaffold regions. A substantial influence of the seeding density was observed on proliferation and osteogenic differentiation of hBMSCs. Thus, the highest proliferation rate and specific alkaline phosphatase activity was found for the cell matrix constructs seeded with the lowest density. RT-PCR analyses revealed a higher expression of alkaline phosphatase and bone sialoprotein II at lower seeding densities; however, expression of osteopontin was unaffected by the seeding cell number. Our results demonstrated that the seeding density might be an important factor for the development of optimal cell matrix constructs for bone tissue engineering.

journal_name

J Tissue Eng Regen Med

authors

Lode A,Bernhardt A,Gelinsky M

doi

10.1002/term.110

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

400-7

issue

7

eissn

1932-6254

issn

1932-7005

journal_volume

2

pub_type

杂志文章
  • Three-dimensional culture of mouse bone marrow cells within a porous polymer scaffold: effects of oxygen concentration and stromal layer on expansion of haematopoietic progenitor cells.

    abstract::To establish an ex vivo expansion method of haematopoietic progenitor cells (HPCs) and erythroid cells, three-dimensional (3D) cultures of mouse bone marrow cells were performed, employing a porous polyvinyl formal (PVF) resin as a scaffold. In these cultures, the effects of oxygen concentration and co-cultures with s...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.295

    authors: Miyoshi H,Murao M,Ohshima N,Tun T

    更新日期:2011-02-01 00:00:00

  • Flow-perfusion bioreactor system for engineered breast cancer surrogates to be used in preclinical testing.

    abstract::There is a need for preclinical testing systems that predict the efficacy, safety and pharmacokinetics of cancer therapies better than existing in vitro and in vivo animal models. An approach to the development of predictive in vitro systems is to more closely recapitulate the cellular and spatial complexity of human ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2026

    authors: Marshall LE,Goliwas KF,Miller LM,Penman AD,Frost AR,Berry JL

    更新日期:2017-04-01 00:00:00

  • Fetal subcutaneous cells have potential for autologous tissue engineering.

    abstract::Major congenital malformations affect up to 3% of newborns. Infants with prenatally diagnosed soft tissue defects should benefit from having autologous tissue readily available for surgical implantation in the perinatal period. In this study, we investigate fetal subcutaneous cells as cellular source for tissue engine...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2639

    authors: Ekblad Å,Westgren M,Fossum M,Götherström C

    更新日期:2018-05-01 00:00:00

  • Microwave-induced porosity and bioactivation of chitosan-PEGDA scaffolds: morphology, mechanical properties and osteogenic differentiation.

    abstract::In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration. In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2241

    authors: Demitri C,Giuri A,De Benedictis VM,Raucci MG,Giugliano D,Sannino A,Ambrosio L

    更新日期:2017-01-01 00:00:00

  • Benefits of concurrent delivery of hyaluronan and IGF-1 cues to regeneration of crosslinked elastin matrices by adult rat vascular cells.

    abstract::Elastin, a major component of vascular matrices, critically determines vascular mechanics and maintains the quiescence of smooth muscle cells (SMCs). Attempts to regenerate elastin in elastin-compromised blood vessels using tissue-engineering approaches is limited by the unavailability of elastogenic cues to upregulat...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.70

    authors: Kothapalli CR,Ramamurthi A

    更新日期:2008-03-01 00:00:00

  • An in vitro expansion score for tissue-engineering applications with human bone marrow-derived mesenchymal stem cells.

    abstract::Human bone marrow-derived mesenchymal stem cells (MSCs) have limited growth potential in vitro and cease to divide due to replicative senescence, which from a tissue-engineering perspective has practical implications, such as defining the correct starting points for differentiation and transplantation. Time spent in c...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1734

    authors: Bertolo A,Mehr M,Janner-Jametti T,Graumann U,Aebli N,Baur M,Ferguson SJ,Stoyanov JV

    更新日期:2016-02-01 00:00:00

  • Tantalum trabecular metal - addition of human skeletal cells to enhance bone implant interface strength and clinical application.

    abstract::The osteo-regenerative properties of allograft have recently been enhanced by addition of autogenous human bone marrow stromal cells (HBMSCs). Limitations in the use of allograft have prompted the investigation of tantalum trabecular metal (TTM) as a potential alternative. TTM is already in widespread orthopaedic use,...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1525

    authors: Smith JO,Sengers BG,Aarvold A,Tayton ER,Dunlop DG,Oreffo RO

    更新日期:2014-04-01 00:00:00

  • Chondrogenic differentiation of human bone marrow mesenchymal stem cells in chitosan-based scaffolds using a flow-perfusion bioreactor.

    abstract::Native articular cartilage is subjected to synovial fluid flow during normal joint function. Thus, it is believed that the morphogenesis of articular cartilage may be positively regulated by the application of similar stimulation in vitro. In the present study, the effect of fluid flow over the chondrogenic differenti...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.372

    authors: Alves da Silva ML,Martins A,Costa-Pinto AR,Correlo VM,Sol P,Bhattacharya M,Faria S,Reis RL,Neves NM

    更新日期:2011-10-01 00:00:00

  • Effects of designed PLLA and 50:50 PLGA scaffold architectures on bone formation in vivo.

    abstract::Biodegradable porous scaffolds have been investigated as an alternative approach to current metal, ceramic, and polymer bone graft substitutes for lost or damaged bone tissues. Although there have been many studies investigating the effects of scaffold architecture on bone formation, many of these scaffolds were fabri...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.497

    authors: Saito E,Liao EE,Hu WW,Krebsbach PH,Hollister SJ

    更新日期:2013-02-01 00:00:00

  • Asymmetrical seeding of MSCs into fibrin-poly(ester-urethane) scaffolds and its effect on mechanically induced chondrogenesis.

    abstract::Mesenchymal stem cells (MSCs) are currently being investigated as candidate cells for regenerative medicine approaches for the repair of damaged articular cartilage. For these cells to be used clinically, it is important to understand how they will react to the complex loading environment of a joint in vivo. In additi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2194

    authors: Gardner OFW,Musumeci G,Neumann AJ,Eglin D,Archer CW,Alini M,Stoddart MJ

    更新日期:2017-10-01 00:00:00

  • Tonsil-derived mesenchymal stem cells (T-MSCs) prevent Th17-mediated autoimmune response via regulation of the programmed death-1/programmed death ligand-1 (PD-1/PD-L1) pathway.

    abstract::Our knowledge of the immunomodulatory role of mesenchymal stem cells (MSCs) in both the innate and adaptive immune systems has dramatically expanded, providing great promise for treating various autoimmune diseases. However, the contribution of MSCs to Th17-dominant immune disease, such as psoriasis and its underlying...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2423

    authors: Kim JY,Park M,Kim YH,Ryu KH,Lee KH,Cho KA,Woo SY

    更新日期:2018-02-01 00:00:00

  • Phenotype-based selection of bone marrow mesenchymal stem cell-derived smooth muscle cells for elastic matrix regenerative repair in abdominal aortic aneurysms.

    abstract::Chronic proteolytic disruption of elastic fibres within the abdominal aortic wall results in wall vessel expansion to form rupture-prone abdominal aortic aneurysms (AAA). Arresting AAA growth is not possible as adult vascular smooth muscle cells (SMCs) poorly auto-regenerate and repair elastic fibres. Thus, there is a...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2349

    authors: Swaminathan G,Stoilov I,Broekelmann T,Mecham R,Ramamurthi A

    更新日期:2018-01-01 00:00:00

  • Numerical assessment on the effective mechanical stimuli for matrix-associated metabolism in chondrocyte-seeded constructs.

    abstract::The self-regeneration capacity of articular cartilage is limited, due to its avascular and aneural nature. Loaded explants and cell cultures demonstrated that chondrocyte metabolism can be regulated via physiologic loading. However, the explicit ranges of mechanical stimuli that correspond to favourable metabolic resp...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.307

    authors: Tasci A,Ferguson SJ,Büchler P

    更新日期:2011-03-01 00:00:00

  • Low-oxygen conditions promote synergistic increases in chondrogenesis during co-culture of human osteoarthritic stem cells and chondrocytes.

    abstract::There has been increased interest in co-cultures of stem cells and chondrocytes for cartilage tissue engineering as there are the limitations associated with using either cell type alone. Drawbacks associated with the use of chondrocytes include the limited numbers of cells available for isolation from damaged or dise...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2608

    authors: Critchley SE,Eswaramoorthy R,Kelly DJ

    更新日期:2018-04-01 00:00:00

  • Controlled collagen crosslinking process in tissue-engineered fibroblast sheets for preventing scar contracture on the surface of lungs.

    abstract::For preventing the scar contracture of host tissue and adjusting the tensile strength of covering cell sheets, a controlled collagen crosslinking step process in the preparation of skin-fibroblast sheets for repairing wound was investigated by using β-aminopropionitrile (BAPN), a collagen crosslinking inhibitor, in th...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.533

    authors: Kanzaki M,Yamato M,Takagi R,Kikkawa T,Isaka T,Okano T,Onuki T

    更新日期:2013-05-01 00:00:00

  • Optimal timing and frequency of bone marrow soup therapy for functional restoration of salivary glands injured by single-dose or fractionated irradiation.

    abstract::Injections of bone marrow (BM) cell extract, known as 'BM soup', were previously reported to mitigate ionizing radiation (IR) injury to salivary glands (SGs). However, the optimal starting time and frequency to maintain BM soup therapeutic efficacy remains unknown. This study tested the optimal starting time and frequ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2513

    authors: Fang D,Shang S,Liu Y,Bakkar M,Sumita Y,Seuntjens J,Tran SD

    更新日期:2018-02-01 00:00:00

  • Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications.

    abstract::Many studies have highlighted the role of silicon in human bone formation and maintenance. Silicon, in fact, is considered to nucleate the precipitation of hydroxyapatite and to reduce the bone resorption. For this reason, we have combined silk fibroin (SF) with silicon-releasing diatom particles (DPs), as potential m...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2373

    authors: Le TDH,Liaudanskaya V,Bonani W,Migliaresi C,Motta A

    更新日期:2018-01-01 00:00:00

  • Enhancement of bone regeneration with the accordion technique via HIF-1α/VEGF activation in a rat distraction osteogenesis model.

    abstract::Axial micromotion of bone fragments promotes callus formation and bone healing during the process of distraction osteogenesis (DO). This study investigated the effects of the combined axial compression and distraction (accordion) technique on bone regeneration in rat DO model. Male Sprague-Dawley rats (n = 62) underwe...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2534

    authors: Xu J,Sun Y,Wu T,Liu Y,Shi L,Zhang J,Kang Q,Chai Y,Li G

    更新日期:2018-02-01 00:00:00

  • Transbuccal platform for delivery of lipogenic actives to facial skin: Because fat matters.

    abstract::The ability to control facial skin physiology and appearance through the oral mucosa (transbuccally) is largely unexplored. Here, a hypothesis was tested that transbuccal delivery of fat tissue-supportive actives may trigger beneficial cosmetic responses at the level of the skin. First, the importance of the fat tissu...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type:

    doi:10.1002/term.3087

    authors: Bojanowski K,Ma S,Applebaum R,Zhao H

    更新日期:2020-08-01 00:00:00

  • The influence of sustained dual-factor presentation on the expansion and differentiation of neural progenitors in affinity-binding alginate scaffolds.

    abstract::Biomaterials capable of controlling the release of multiple growth factors (GFs) could potentially promote the integration of co-transplanted neural progenitor cells (NPCs) and stimulate the plasticity and regenerability of the lesioned spinal cord. As a first step towards the employment of such a vehicle for cell the...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1797

    authors: Cizkova D,Slovinska L,Grulova I,Salzet M,Cikos S,Kryukov O,Cohen S

    更新日期:2015-08-01 00:00:00

  • Mesenchymal stem cells protect from acute liver injury by attenuating hepatotoxicity of liver natural killer T cells in an inducible nitric oxide synthase- and indoleamine 2,3-dioxygenase-dependent manner.

    abstract::The effects of mesenchymal stem cells (MSCs) on the phenotype and function of natural killer T (NKT) cells is not understood. We used concanavalin A (Con A) and α-galactosylceramide (α-GalCer)-induced liver injury to evaluate the effects of MSCs on NKT-dependent hepatotoxicity. Mouse MSCs (mMSCs) significantly reduced...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2452

    authors: Gazdic M,Simovic Markovic B,Vucicevic L,Nikolic T,Djonov V,Arsenijevic N,Trajkovic V,Lukic ML,Volarevic V

    更新日期:2018-02-01 00:00:00

  • Dendrimers: Breaking the paradigm of current musculoskeletal autoimmune therapies.

    abstract::The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2597

    authors: Rodrigues DB,Oliveira JM,Santos TC,Reis RL

    更新日期:2018-03-01 00:00:00

  • Enhancing human nucleus pulposus cells for biological treatment approaches of degenerative intervertebral disc diseases: a systematic review.

    abstract::Intervertebral disc (IVD) degeneration has been described as an aberrant, cell-mediated, age- and genetics-dependent molecular degeneration process, which can be accelerated by nutritional, mechanical and toxic factors. Collective involvement of these factors can result in structural failures, which are often associat...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.1583

    authors: Mern DS,Beierfuß A,Thomé C,Hegewald AA

    更新日期:2014-12-01 00:00:00

  • Photopolymerized micropatterns with high feature frequencies overcome chemorepulsive borders to direct neurite growth.

    abstract::Developing and regenerating neurites respond to a variety of biophysical and biochemical cues in their micro-environment to reach target cells and establish appropriate synapses. Defining the hierarchal relationship of both types of cues to direct neurite growth carries broad significance for neural development, regen...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2527

    authors: Tuft BW,Xu L,Leigh B,Lee D,Guymon CA,Hansen MR

    更新日期:2018-03-01 00:00:00

  • Phospholipid-induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration.

    abstract::Silk fibroin (SF) hydrogels can be obtained via self-assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) sodium salt (DMPG), was used to induce and accelerate the gelatio...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2982

    authors: Laomeephol C,Guedes M,Ferreira H,Reis RL,Kanokpanont S,Damrongsakkul S,Neves NM

    更新日期:2020-01-01 00:00:00

  • 3D ingrowth of bovine articular chondrocytes in biodegradable cryogel scaffolds for cartilage tissue engineering.

    abstract::A feasibility study was undertaken to examine the potential of biodegradable HEMA-lactate-dextran (HEMA-LLA-D)-based cryogels as scaffolds for cartilage tissue engineering. This was a preliminary in vitro study giving essential information on the biocompatibility of cryogels with cartilage cells. HEMA-lactate (HEMA-LL...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.375

    authors: Bölgen N,Yang Y,Korkusuz P,Güzel E,El Haj AJ,Pişkin E

    更新日期:2011-11-01 00:00:00

  • A collagen cardiac patch incorporating alginate microparticles permits the controlled release of hepatocyte growth factor and insulin-like growth factor-1 to enhance cardiac stem cell migration and proliferation.

    abstract::Cardiac stem cells (CSCs) represent a logical cell type to exploit as a regenerative treatment option for tissue damage accrued as a result of a myocardial infarction. However, the isolation and expansion of CSCs prior to cell transplantation is time consuming, costly and invasive, and the reliability of cell expansio...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2392

    authors: O'Neill HS,O'Sullivan J,Porteous N,Ruiz-Hernandez E,Kelly HM,O'Brien FJ,Duffy GP

    更新日期:2018-01-01 00:00:00

  • Developing palatal bone using human mesenchymal stem cell and stem cells from exfoliated deciduous teeth cell sheets.

    abstract::Cleft palate is one of the most common craniofacial defects in newborn babies. The characteristics of this genetic disease produce soft and hard tissue defects on the lip and maxilla, which cause not only aesthetic but also functional problems with speech, eating, and breathing. Bone grafts using autologous cancellous...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2811

    authors: Lee JM,Kim HY,Park JS,Lee DJ,Zhang S,Green DW,Okano T,Hong JH,Jung HS

    更新日期:2019-02-01 00:00:00

  • Biodegrading highly porous elastomeric graft regenerates muscular and innervated carotid artery-Comparative study with vein graft.

    abstract::This study aims to investigate the superiorities of fast degrading elastomeric poly(glycerol sebacate) (PGS)/polycaprolactone (PCL) grafts over autologous vein grafts in the reconstruction of carotid artery, thus providing more suitable vascular grafts for carotid artery replacement. We fabricated small arterial graft...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2856

    authors: Yang X,Gao Z,Liu H,Wu W

    更新日期:2019-07-01 00:00:00

  • Low-intensity pulsed ultrasound combination with induced pluripotent stem cells-derived neural crest stem cells and growth differentiation factor 5 promotes sciatic nerve regeneration and functional recovery.

    abstract::The treatment of lengthy peripheral nerve defect is challenging in the field of nerve regeneration. Our previous studies have shown that low-intensity pulsed ultrasound (LIPUS) could promote the proliferation, cell viability, and neural differentiation of induced pluripotent stem cells-derived neural crest stem cells ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2823

    authors: Xia B,Chen G,Zou Y,Yang L,Pan J,Lv Y

    更新日期:2019-04-01 00:00:00