Abstract:
:Islet replacement is a promising strategy for the treatment of patients with type 1 diabetes and patients who have undergone total pancreatectomy. Recent progress in cellular reprogramming technology may allow the transplantation of a patient's own pancreatic cells. Although many studies have reported the differentiation of pancreatic progenitor cells from mouse and human pluripotent stem cells (PSCs), obtaining sufficient cell numbers for clinical applications remains problematic. Here, we describe the mass production of human pancreatic progenitor cells from human induced (i)PSCs using a three-dimensional suspension bioreactor system. Bioreactor culture of cells with stage-specific provision of growth factors and small compounds for 17 days produced approximately 1.6 × 108 cells/100 ml vessel in a single batch. About 95% of cells expressed pancreatic and duodenal homeobox factor 1, and 22% co-expressed the transcription factor NKX6.1. Furthermore, culture of pancreatic progenitor cells for an additional 2 weeks yielded mature pancreatic cells, including C-peptide-, glucagon- and trypsin-expressing cell populations. Moreover, differentiated β-cells secreted insulin in response to increased glucose in vitro. These findings suggest that a three-dimensional suspension culture system can generate human pancreatic progenitor cells from human iPSCs. Further optimization of culture conditions should provide sufficient functional islet cells for transplantation therapy. Copyright © 2017 John Wiley & Sons, Ltd.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Mihara Y,Matsuura K,Sakamoto Y,Okano T,Kokudo N,Shimizu Tdoi
10.1002/term.2228subject
Has Abstractpub_date
2017-11-01 00:00:00pages
3193-3201issue
11eissn
1932-6254issn
1932-7005journal_volume
11pub_type
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2010-07-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2017-02-01 00:00:00
abstract::Adipose tissue-derived microvascular fragments (ad-MVFs) are promising vascularization units for tissue engineering. In this study, we analysed the effects of normothermic (37°C) and subnormothermic (20°C) short-term cultivation on their viability and network forming capacity. Ad-MVFs from green fluorescent protein (G...
journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2019-02-01 00:00:00
abstract::In this study, a simple three-dimensional (3D) suspension culture method for the expansion and cardiac differentiation of human induced pluripotent stem cells (hiPSCs) is reported. The culture methods were easily adapted from two-dimensional (2D) to 3D culture without any additional manipulations. When hiPSCs were dir...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1761
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2337
更新日期:2018-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are currently being investigated as candidate cells for regenerative medicine approaches for the repair of damaged articular cartilage. For these cells to be used clinically, it is important to understand how they will react to the complex loading environment of a joint in vivo. In additi...
journal_title:Journal of tissue engineering and regenerative medicine
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doi:10.1002/term.2194
更新日期:2017-10-01 00:00:00
abstract::Fracture haematoma formation is the first and foremost important stage of fracture healing. It orchestrates the inflammatory and cellular processes leading to the formation of callus and the restoration of the continuity of the bone. Evidence suggests that blocking this initial stage could lead to an impairment of the...
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