Abstract:
:Bone marrow stromal cells (BMSC) transplantation enhances functional recovery after cerebral infarct, but the optimal delivery route is undetermined. This study was aimed to assess whether a novel cell-sheet technology non-invasively serves therapeutic benefits to ischemic stroke. First, the monolayered cell sheet was engineered by culturing rat BMSCs on a temperature-responsive dish. The cell sheet was analysed histologically and then transplanted onto the ipsilateral neocortex of rats subjected to permanent middle cerebral artery occlusion at 7 days after the insult. Their behaviours and histology were compared with those in the animals treated with direct injection of BMSCs or vehicle over 4 weeks post-transplantation. The cell sheet was 27.9 ± 8.0 μm thick and was composed of 9.8 ± 2.4 × 105 cells. Cell sheet transplantation significantly improved motor function when compared with the vehicle-injected animals. Histological analysis revealed that the BMSCs were densely distributed to the neocortex adjacent to the cerebral infarct and expressed neuronal phenotype in the cell sheet-transplanted animals. These findings were almost equal to those for the animals treated with direct BMSC injection. The attachment of the BMSC sheet to the brain surface did not induce reactive astrocytes in the adjacent neocortex, although direct injection of BMSCs profoundly induced reactive astrocytes around the injection site. These findings suggest that the BMSCs in cell sheets preserve their biological capacity of migration and neural differentiation. Cell-sheet technology may enhance functional recovery after ischaemic stroke, using a less invasive method. Copyright © 2014 John Wiley & Sons, Ltd.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Ito M,Shichinohe H,Houkin K,Kuroda Sdoi
10.1002/term.1920subject
Has Abstractpub_date
2017-02-01 00:00:00pages
375-381issue
2eissn
1932-6254issn
1932-7005journal_volume
11pub_type
杂志文章abstract::Axial micromotion of bone fragments promotes callus formation and bone healing during the process of distraction osteogenesis (DO). This study investigated the effects of the combined axial compression and distraction (accordion) technique on bone regeneration in rat DO model. Male Sprague-Dawley rats (n = 62) underwe...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
abstract::Manufacture of red blood cells (RBCs) from progenitors has been proposed as a method to reduce reliance on donors. Such a process would need to be extremely efficient for economic viability given a relatively low value product and high (2 × 1012 ) cell dose. Therefore, the aim of these studies was to define the produc...
journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.3
更新日期:2007-01-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1700
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.496
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.67
更新日期:2008-01-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.141
更新日期:2009-02-01 00:00:00
abstract::Bone-patellar tendon-bone (BPTB) autografts are the gold standard for anterior cruciate ligament (ACL) reconstruction because the bony ends allow for superior healing and anchoring through bone-to-bone regeneration. However, the disadvantages of BPTB grafts include donor site morbidity and patellar rupture. In order t...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1966
更新日期:2017-03-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2377
更新日期:2018-01-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.40
更新日期:2007-07-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
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更新日期:2017-11-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.156
更新日期:2009-03-01 00:00:00
abstract::Glaucoma, a leading cause of blindness, is characterized by an increase in intraocular pressure, which is largely determined by resistance to aqueous humour outflow through the trabecular meshwork (TM). In glaucoma, the cellularity of the TM is decreased, and, as a result, stem cell therapies for the TM represent a po...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2488
更新日期:2018-03-01 00:00:00
abstract::Prevascularization is essential to ensure the viability, functionality, and successful integration of tissue-engineered three-dimensional (3D) constructs with surrounding host tissues after transplantation. Human mesenchymal stem cell (hMSC) sheet can be prevascularized by coculturing with endothelial cells (ECs), and...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3167
更新日期:2021-01-11 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2454
更新日期:2018-03-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1878
更新日期:2015-07-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
abstract::The composition and organization of the vocal fold extracellular matrix (ECM) provide the viscoelastic mechanical properties that are required to sustain high-frequency vibration during voice production. Although vocal injury and pathology are known to produce alterations in matrix physiology, the mechanisms responsib...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
abstract::Previous researches have emphasized on suitability of decellularized tissues for regenerative applications. The decellularization of cartilage tissue has always been a challenge as the final product must be balanced in both immunogenic residue and mechanical properties. This study was designed to compare and optimize ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2019-10-01 00:00:00
abstract::The ability to control facial skin physiology and appearance through the oral mucosa (transbuccally) is largely unexplored. Here, a hypothesis was tested that transbuccal delivery of fat tissue-supportive actives may trigger beneficial cosmetic responses at the level of the skin. First, the importance of the fat tissu...
journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2020-08-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00