Abstract：
:Ex vivo lung perfusion (EVLP) allows perfusion and reconditioning of retrieved lungs for organ transplantation. The Steen solution is specifically designed for this procedure but the mechanism through which it elicits its activity is still to be fully clarified. We speculated that Steen solution may encompass antioxidant properties allowing a reestablishment of pulmonary tissue homeostasis. Blood samples from 10 healthy volunteers were recruited. Platelets and white cells were incubated with Steen solution or buffer solution as control and stimulated with suitable agonists. Reactive oxidant species (ROS), soluble NOX2 (sNOX2-derived peptide), a marker of NADPH oxidase activation, p47(phox) translocation to cell membrane and isoprostanes production, as marker of oxidative stress, and nitric oxide (NO), a powerful vasodilator and antioxidant molecule, were measured upon cell stimulation. The Steen solution significantly inhibited p47(phox) translocation and NOX2 activation in platelets and white cells. Consistent with this finding was the reduction of oxidative stress as documented by a significantly lowered formation of ROS and isoprostanes by both platelets and white cells. Finally, cell incubation with Steen solution resulted in enhanced generation of NO. Herewith, we provide the first evidence that Steen solution possesses antioxidant properties via downregulation of NADPH oxidase activity and enhanced production of NO.
journal_name
Oxid Med Cell Longevjournal_title
Oxidative medicine and cellular longevityauthors
Carnevale R,Biondi-Zoccai G,Peruzzi M,De Falco E,Chimenti I,Venuta F,Anile M,Diso D,Cavarretta E,Marullo AG,Sartini P,Pignatelli P,Violi F,Frati Gdoi
10.1155/2014/242180subject
Has Abstractpub_date
2014-01-01 00:00:00pages
242180eissn
1942-0900issn
1942-0994journal_volume
2014pub_type
杂志文章abstract：SCOPE:To determine whether doses of quercetin in the range of serum concentrations exert any effect on triacylglycerol accumulation in maturing preadipocytes and mature adipocytes. The influence on the expression of adipogenic markers as well as on gene expression and activity of enzymes involved in triacylglycerol met...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/480943
更新日期：2015-01-01 00:00:00
abstract：:Hyperammonemia is a serious complication of liver disease and may lead to encephalopathy and death. This study investigated the effects of Commiphora molmol resin on oxidative stress, inflammation, and hematological alterations in ammonium chloride- (NH4Cl-) induced hyperammonemic rats, with an emphasis on the glutama...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/7369671
更新日期：2017-01-01 00:00:00
abstract：:A number of drugs have been withdrawn from the market or severely restricted in their use because of unexpected toxicities that become apparent only after the launch of new drug entities. Circumstantial evidence suggests that, in most cases, reactive metabolites are responsible for these unexpected toxicities. In this...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.4161/oxim.3.4.13246
更新日期：2010-07-01 00:00:00
abstract：:The human apoptosis-inducing factor (hAIF) is a moonlight flavoprotein involved in mitochondrial respiratory complex assembly and caspase-independent programmed cell death. These functions might be modulated by its redox-linked structural transition that enables hAIF to act as a NAD(H/+) redox sensor. Upon reduction w...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2021/6673661
更新日期：2021-01-15 00:00:00
abstract：:Ischemic stroke remains the fifth cause of death, as reported worldwide annually. Endothelial dysfunction (ED) manifesting with lower nitric oxide (NO) bioavailability leads to increased vascular tone, inflammation, and platelet activation and remains among the major contributors to cardiovascular diseases (CVD). More...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2020/2979260
更新日期：2020-08-28 00:00:00
abstract：:This study aimed to elucidate the determinants of higher plasma malondialdehyde (MDA) in free-living adults. In a cross-sectional study we evaluated 148 free-living subjects (54 ± 11 years, 78% women) at high risk for or with metabolic syndrome (MetS). They were assessed by anthropometry and body composition, dietary ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2014/505368
更新日期：2014-01-01 00:00:00
abstract：:Edaravone (5-methyl-2-phenyl-2,4-dihydro-3H-pyrazol-3-one, EDV) is a free-radical scavenger reduces organ ischemic injury. Here we investigated whether the protective effects of EDV in renal ischemia/reperfusion (I/R) injury may be enhanced by an EDV derivative bearing a nitric oxide- (NO-) donor furoxan moiety (NO-ED...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/804659
更新日期：2015-01-01 00:00:00
abstract：:There is little research on how cooking and digestion affect the anti-inflammatory activity of culinary herbs. Thus, the aim of this paper was to investigate this activity following cooking and in vitro digestion of the common culinary herbs, rosemary, sage, and thyme, and the relationship between their anti-inflammat...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2012/627843
更新日期：2012-01-01 00:00:00
abstract：Aims:Oxidative stress is a factor involved in the pathogenesis of celiac disease (CD), possibly affecting the course of the disease and celiac-related complications. We assessed the intensity of oxidative processes and the efficiency of antioxidant defense in children with celiac disease. Methods. Group I (n = 32) cons...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/1324820
更新日期：2018-05-02 00:00:00
abstract：:Glucocorticoid (GC) production is physiologically regulated through a negative feedback loop mediated by the GC, which appear disrupted in several pathological conditions. The inability to perform negative feedback of the hypothalamus-pituitary-adrenal (HPA) axis in several diseases is associated with an overproductio...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/4156361
更新日期：2017-01-01 00:00:00
abstract：:As a member of peroxiredoxin (Prx) family, PrxIII has been demonstrated to play an important role in scavenging intracellular reactive oxygen species (ROS). Since PrxIII knockout mice exhibited oxidative stress in placentas resembling pathophysiologic changes in placentas of human pre-eclampsia, we measured blood pres...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.4161/oxim.3.1.10094
更新日期：2010-01-01 00:00:00
abstract：:The human thymus is a primary lymphoepithelial organ which supports the production of self-tolerant T cells with competent and regulatory functions. Paradoxically, despite the crucial role that it exerts in T cell-mediated immunity and prevention of systemic autoimmunity, the thymus is the first organ of the body that...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2020/7986071
更新日期：2020-02-05 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia, characterized by progressive neurodegeneration. Pathogenetic mechanisms, triggered by β-amyloid (Aβ) accumulation, include oxidative stress, derived from energy homeostasis deregulation and involving mitochondria and peroxisomes. We here addressed the oxida...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/967203
更新日期：2015-01-01 00:00:00
abstract：:Since excessive reactive oxygen species (ROS) is known to be associated with aging and age-related diseases, strategies modulating ROS level and antioxidant defense systems may contribute to the delay of senescence. Here we show that the protein hydrolyzate from Angelica sinensis was capable of increasing oxidative su...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/8956981
更新日期：2016-01-01 00:00:00
abstract：:The main properties and biological effects of the antioxidant carnosine, the natural dipeptide β-alanyl-L-histidine, are considered. Data on the effective use of carnosine in different pathologies are presented. Special attention is paid to issues of use of carnosine in neurologic and mental diseases, in alcoholism as...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/2939087
更新日期：2016-01-01 00:00:00
abstract：:This study addressed the relationship of proinflammatory cytokines and Nrf2-Keap1 system in diabetic nephropathy. The experimental groups were control, diabetic, and diabetic treated with mycophenolate mofetil (MMF). The renal function, proinflammatory and profibrotic cytokines, oxidative stress, morphology, and nephr...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/4693801
更新日期：2016-01-01 00:00:00
abstract：:Inflammation and oxidative stress are pivotal mechanisms for the pathogenesis of ischemia and reperfusion injury (IRI). Vagus nerve stimulation (VNS) may participate in maintaining oxidative homeostasis and response to external stimulus or injury. We investigated whether the in vivo VNS can protect the liver from IRI....
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/1079129
更新日期：2020-01-21 00:00:00
abstract：:Fungal immunomodulatory proteins (FIPs) are a class of small proteins that have been extensively studied for their immunomodulatory activities. In this study, two novel FIPs from Lentinus tigrinus were identified and named Fip-lti1 and Fip-lti2. The bioactive characteristics of Fip-lti1 and Fip-lti2 were compared to a...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/3139689
更新日期：2019-05-06 00:00:00
abstract：:The neutrophil elastase inhibitor sivelestat (ONO-5046) possesses unknown mechanisms of cardioprotection when infused following global ischemia, even in the absence of neutrophils. Since myocardial ischemia-reperfusion injury is strongly associated with endothelial dysfunction and reactive oxygen species (ROS) generat...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/279847
更新日期：2013-01-01 00:00:00
abstract：:Virulence of enterohemorrhagic Escherichia coli (EHEC) strains depends on production of Shiga toxins. These toxins are encoded in genomes of lambdoid bacteriophages (Shiga toxin-converting phages), present in EHEC cells as prophages. The genes coding for Shiga toxins are silent in lysogenic bacteria, and prophage indu...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/3578368
更新日期：2016-01-01 00:00:00
abstract：:Doxorubicin (DOX) is an efficient chemotherapeutic agent, but its clinical application is limited by its cardiotoxicity associated with increased oxidative stress. Thus, the combination of DOX and antioxidants has been encouraged. In this study, we evaluated (I) the chemical composition and antioxidant capacity of aqu...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/2935051
更新日期：2018-06-28 00:00:00
abstract：Background/Aims:Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by abnormal hormone levels in peripheral blood and poor-quality oocytes. PCOS is a pathophysiological syndrome caused by chronic inflammation and oxidative stress. The aim of this study was to investigate the mechanism of melatonin ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/8218650
更新日期：2019-10-20 00:00:00
abstract：:Xeroderma pigmentosum group A (XPA) is a genetic disorder in DNA nucleotide excision repair (NER) with severe neurological disorders, in which oxidative stress and disturbed melatonin metabolism may be involved. Herein we confirmed the diurnal variation of melatonin metabolites, oxidative stress markers, and antioxida...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/5741517
更新日期：2016-01-01 00:00:00
abstract：:2-[(2,6-Dichlorobenzylidene)amino]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile, 5TIO1, is a new 2-aminothiophene derivative with promising pharmacological activities. The aim of this study was to evaluate its antioxidant activity in different areas of mice central nervous system. Male Swiss adult mice were in...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/194192
更新日期：2013-01-01 00:00:00
abstract：:Oxidative stress reflects an imbalance between oxidants and antioxidants in favor of the oxidants capable of evoking tissue damage. Like hydrogen sulfide (H2S) and nitric oxide (NO), carbon monoxide (CO) is an endogenous gaseous mediator recently implicated in the physiology of the gastrointestinal (GI) tract. CO is p...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2020/5083876
更新日期：2020-04-15 00:00:00
abstract：:With recent insight into the mechanisms involved in diseases, such as cardiovascular disease, cancer, stroke, neurodegenerative diseases, and diabetes, more efficient modes of treatment are now being assessed. Traditional medicine including the use of natural products is widely practiced around the world, assuming tha...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2015/181260
更新日期：2015-01-01 00:00:00
abstract：:MicroRNAs are short (21-23 nucleotides), noncoding RNAs that typically silence posttranscriptional gene expression through interaction with target messenger RNAs. Currently, miRNAs have been identified in almost all studied multicellular eukaryotes in the plant and animal kingdoms. Additionally, recent studies reporte...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/6021934
更新日期：2016-01-01 00:00:00
abstract：:The role of altered redox status and high reactive oxygen species (ROS) is still controversial in cancer development and progression. Intracellular levels of ROS are elevated in cancer cells suggesting a role in cancer initiation and progression; on the contrary, ROS elevated levels may induce programmed cell death an...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2017/2597581
更新日期：2017-01-01 00:00:00
abstract：:Renal ischemia-reperfusion injury (RIRI) refers to a phenomenon associated with dysfunction of the kidney and tissue damage. Unfortunately, no specific drugs have been found that effectively prevent and treat RIRI. Curcumin (Cur), a polyphenol extracted from turmeric, possesses a variety of biological activities invol...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/4546851
更新日期：2020-11-12 00:00:00
abstract：:This work reviews concepts regarding oxidative stress and the mechanisms by which endogenous and exogenous factors produce reactive oxygen species (ROS). It also surveys the relationships between oxidative stress, circadian rhythms, and retinal damage in humans, particularly those related to light and photodamage. In ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/7420637
更新日期：2016-01-01 00:00:00
