Heme-related molecules induce rapid production of neutrophil extracellular traps.

Abstract:

BACKGROUND:Pulmonary endothelial cell damages caused by neutrophil overactivation could result in acute lung injuries including transfusion-related acute lung injury (TRALI). We previously reported that heme-related molecules derived from hemolysis induced the production of reactive oxygen species from neutrophils. Recently, neutrophil extracellular traps (NETs) have been demonstrated to associate with the onset of TRALI. STUDY DESIGN AND METHODS:In this study, neutrophils' morphologic changes induced by the heme-related molecule hemin were confirmed to be NETs via confocal laser scanning microscopy and electron microscopy (EM). Additionally, concentrations of hemin in red blood cell (RBC) components were measured via enzyme-linked immunosorbent assay and possible contribution of these molecules to the onset of TRALI was discussed. RESULTS:SYTOX green staining observation via confocal laser scanning microscopy revealed that neutrophil morphology changed rapidly upon addition of hemin. The nuclei began to be enlarged and become segmented after 5 minutes, and NET-like structures were released from neutrophils after 15 minutes. In EM observation, NET-like structures appeared after 10 minutes and the nucleoplasm was partially separated from the nuclear membrane, which were consistent with the features of NET formation. These structures stained positively for both myeloperoxidase and histone H3 antibodies. CONCLUSION:Thus, our results suggest that hemin induced NETs in 15 minutes, a quicker reaction than NET induction by phorbol myristate acetate requiring 3 hours. Moreover, since RBC components, especially those with long-term storage, contained sufficient hemin concentration to induce NETs, special attention to hemolysis of stored RBC components is important.

journal_name

Transfusion

journal_title

Transfusion

authors

Kono M,Saigo K,Takagi Y,Takahashi T,Kawauchi S,Wada A,Hashimoto M,Minami Y,Imoto S,Takenokuchi M,Morikawa T,Funakoshi K

doi

10.1111/trf.12700

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

2811-9

issue

11

eissn

0041-1132

issn

1537-2995

journal_volume

54

pub_type

临床试验,杂志文章,多中心研究
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    abstract:BACKGROUND:The Le(a) and Le(b) antigens are synthesized by cells making exocrine secretions, but it is not well-defined where plasma and red cell Le(a) and Le(b) antigens are made. STUDY DESIGN AND METHODS:This study uses chromatographic and immunochemical methods to extract Le(a) and Le(b) glycolipids from human tiss...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1995.35795357880.x

    authors: Hauser R

    更新日期:1995-07-01 00:00:00

  • Impact of cytomegalovirus (CMV) antibody reflex testing in the transfusion service on management of CMV-seronegative blood inventory.

    abstract:BACKGROUND:Our goal is to minimize unnecessary cytomegalovirus (CMV)-seronegative blood transfusion to preserve the CMV-seronegative blood inventory for patients who are identified as CMV seronegative. STUDY DESIGN AND METHODS:We implemented a CMV antibody reflex testing protocol for patients who require CMV-compatibl...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02643.x

    authors: Fontaine MJ,Jurado C,Miller E,Viele M,Goodnough LT

    更新日期:2010-08-01 00:00:00

  • Residual plasma in red blood cells and transfusion-related acute lung injury.

    abstract:BACKGROUND:Transfusion-related acute lung injury (TRALI) is the most common cause of death from blood transfusion and red blood cells (RBCs) now account for approximately 50% of these fatalities. RBCs from female donors have been implicated in large series and HLA Class II antibodies to cognate recipient antigens ident...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12662

    authors: Weber LL,Roberts LD,Sweeney JD

    更新日期:2014-10-01 00:00:00

  • The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers.

    abstract:BACKGROUND:Human parvovirus B19 is a common human pathogen that causes a variety of diseases with outcomes ranging from asymptomatic to severe, especially in immunocompromised patients. The B19 virus can be transmitted via blood and/or blood products and its resistance to common viral inactivation and/or removal method...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03067.x

    authors: Ke L,He M,Li C,Liu Y,Gao L,Yao F,Li J,Bi X,Lv Y,Wang J,Hirsch ML,Li W

    更新日期:2011-09-01 00:00:00

  • Prospective surveillance of D- recipients of D+ apheresis platelets: alloimmunization against D is not detected.

    abstract:BACKGROUND:Recent retrospective studies indicate that D- recipients of D+ apheresis platelets (PLTs) are not alloimmunized to D. Our hospital policy is to offer RhIG to D- women of childbearing age who received D+ apheresis PLTs but not to other D- recipients of D+ apheresis PLTs. We instituted prospective surveillance...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12972

    authors: Weinstein R,Simard A,Ferschke J,Vauthrin M,Bailey JA,Greene M

    更新日期:2015-06-01 00:00:00

  • Convenience, the bane of our existence, and other barriers to donating.

    abstract:BACKGROUND:To prevent donor loss and improve retention, it is important to understand the major deterrents to blood donation and to identify factors that can be effectively addressed by blood centers. STUDY DESIGN AND METHODS:A 30-item self-administered questionnaire was completed in 2003 by 1705 first-time and 2437 r...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.00757.x

    authors: Schreiber GB,Schlumpf KS,Glynn SA,Wright DJ,Tu Y,King MR,Higgins MJ,Kessler D,Gilcher R,Nass CC,Guiltinan AM,National Heart, Lung, Blood Institute Retrovirus Epidemiology Donor Study.

    更新日期:2006-04-01 00:00:00

  • Improved removal of white cells with minimal platelet loss by filtration of apheresis platelets during collection.

    abstract:BACKGROUND:Filtration of apheresis platelets to remove white cells (WBCs) requires operator intervention after the collection procedure (postcollection filtration), which may cause variable and unsatisfactory filter performance (WBC removal and platelet loss). The MCS+ LN9000 apheresis system filters platelets through ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1999.39199116898.x

    authors: Holme S,Andres M,Goermar N,Giordano GF

    更新日期:1999-01-01 00:00:00

  • Transfusion of ABO-nonidentical platelets is not associated with adverse clinical outcomes in cardiovascular surgery patients.

    abstract:BACKGROUND:Current blood transfusion standards in Canada and the United States permit transfusion of ABO-nonidentical platelets when ABO-identical platelets are not available. This practice increases the availability of platelets, a component in chronic shortage in Ontario, Canada because of the 5-day shelf-life. The i...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2002.00037.x

    authors: Lin Y,Callum JL,Coovadia AS,Murphy PM

    更新日期:2002-02-01 00:00:00

  • Effects of UVB radiation on cytokine generation, cell adhesion molecules, and cell activation markers in T-lymphocytes and peripheral blood HPCs.

    abstract:BACKGROUND:Immunomodulatory effects of UV light have increasingly become a focus in transfusion medicine, BMT and transplantation immunology. In the transplant setting, the use of UVB radiation may reduce or abolish T-cell activation without compromising either bone marrow (BM) engraftment or graft-versus-leukemia effe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40030361.x

    authors: Lankford KV,Mosunjac M,Hillyer CD

    更新日期:2000-03-01 00:00:00

  • Improvement of platelet storage conditions by using new polyolefin containers.

    abstract:BACKGROUND:Storage of pooled platelet concentrates (PCs) with yields above 3.0 x 10(11) platelets per unit in a 1-L PL-732 polyolefin container for 5 days often results in a drop in pH to below 6.0. Recently, new oxygen-permeable platelet containers (1-L PL-2410, 1-L and 1.5-L Compoflex) have been developed. The maxima...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1997.37597293876.x

    authors: de Wildt-Eggen J,Schrijver JG,Bouter-Valk HJ,Fijnheer R,Bins M,van Prooijen HC

    更新日期:1997-05-01 00:00:00

  • Transfusion of red cells after autologous bone marrow harvest in patients with acute leukemia and malignant lymphoma.

    abstract::Bone marrow was harvested for the purpose of autologous bone marrow transplantation (ABMT) in 21 patients previously treated with chemotherapy and in complete remission from acute leukemia or non-Hodgkin's lymphoma. The volume required to obtain 2 x 10(8) nucleated cells per kg was less than 15 mL per kg in 13 patient...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30490273437.x

    authors: Sonneveld P,de Leeuw CA,Schipperus M,Lindemans J,Abels J

    更新日期:1990-05-01 00:00:00

  • Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors.

    abstract:BACKGROUND:The correlation between signal-to-cutoff (S/CO) ratios of a second-generation hepatitis C virus (HCV) enzyme immunoassay (EIA; Abbott) and a third-generation HCV enzyme-linked immunosorbent assay (ELISA; Ortho) and confirmed HCV infection has been reported. The utility of the values for the Chinese anti-HCV ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2005.00611.x

    authors: Ren FR,Lv QS,Zhuang H,Li JJ,Gong XY,Gao GJ,Liu CL,Wang JX,Yao FZ,Zheng YR,Zhu FM,Tiemuer MH,Bai XH,Shan H

    更新日期:2005-11-01 00:00:00

  • Reduction in red blood cell transfusion associated with engagement of the ordering physician.

    abstract:BACKGROUND:Data on red blood cell (RBC) transfusion in the United States show variation in practice and overprescribing or overdosing is considered to be prevalent. Education or restrictive interventions could modify practice. STUDY DESIGN AND METHODS:RBC transfusion and mortality rates were recorded in a single insti...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12552

    authors: Tavares MM,Diquattro PJ,Sweeney JD

    更新日期:2014-10-01 00:00:00

  • A rapid method for harvesting autologous red cells from patients with hemoglobin S disease.

    abstract::Red cells from patients with sickle cell disease (HbSS and HbSC) are more resistant to lysis in hypotonic NaCl solutions than normal (HbAA) red cells. Taking advantage of this inherent resistance to osmotic stress, patient red cells (HbSS or HbSC) were rapidly isolated from donor red cells (HbAA or HbAS) by washing wi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1988.28188127945.x

    authors: Brown DJ

    更新日期:1988-01-01 00:00:00

  • Long-term production of pre-existing alloantibodies to E and c after allogenic BMT in a patient with aplastic anemia resulting in delayed hemolytic anemia.

    abstract:BACKGROUND:Delayed hemolysis mediated by long-term production of pre-existing recipient-derived antibodies directed against donor RBC antigens after allogenic BMT is an unusual complication of hematopoietic transplantation. CASE REPORT:A 26-year-old man with aplastic anemia had pre-existing alloantibodies to E and c. ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2003.00303.x

    authors: Izumi N,Fuse I,Furukawa T,Uesugi Y,Tsuchiyama J,Toba K,Togashi K,Yamada K,Ohtake S,Saitoh Y,Yanagisawa N,Aizawa Y

    更新日期:2003-02-01 00:00:00

  • Lengths of hepatitis B viremia and antigenemia in blood donors: preliminary evidence of occult (hepatitis B surface antigen-negative) infection in the acute stage.

    abstract:BACKGROUND:The Japanese Red Cross (JRC) implemented a fully automated pooling and nucleic acid amplification test (NAT) system for testing seronegative donations. The JRC sample repository and repeat blood donations allowed for lookback and follow-up studies of hepatitis B virus (HBV) DNA-positive donors, who tested ne...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01234.x

    authors: Yoshikawa A,Gotanda Y,Minegishi K,Taira R,Hino S,Tadokoro K,Ohnuma H,Miyakawa K,Tachibana K,Mizoguchi H,Japanese Red Cross NAT Screening Research Group.

    更新日期:2007-07-01 00:00:00

  • Improving health profile of blood donors as a consequence of transfusion safety efforts.

    abstract:BACKGROUND:Transfusion safety rests heavily on the health of blood donors. Although they are perceived as being healthier than average, little is known about their long-term disease patterns and to which extent the blood banks' continuous efforts to optimize donor selection has resulted in improvements. Mortality and c...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01425.x

    authors: Edgren G,Tran TN,Hjalgrim H,Rostgaard K,Shanwell A,Titlestad K,Wikman A,Norda R,Jersild C,Wideroff L,Gridley G,Adami J,Melbye M,Nyrén O,Reilly M

    更新日期:2007-11-01 00:00:00

  • Outliers in RhD membrane integration are explained by variant RH haplotypes.

    abstract:BACKGROUND:Variations in a multipass transmembrane protein may affect its membrane integration. To study this effect, the systematic molecular characterization of variant D antigen density is a suitable model. Unlike most other membrane proteins, the expression of the D antigen is often determined by a single allele, b...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.00902.x

    authors: Yu X,Wagner FF,Witter B,Flegel WA

    更新日期:2006-08-01 00:00:00

  • Hemovigilance survey of pathogen-reduced blood components in the Warsaw Region in the 2009 to 2013 period.

    abstract:BACKGROUND:In 2009 the Mirasol Pathogen Reduction Technology (PRT) was introduced to the routine blood component production of the Regional Blood Transfusion Center in Warsaw (RBTCW). The goal of this study was to investigate the safety of Mirasol-treated blood components. STUDY DESIGN AND METHODS:The accumulated pass...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.13330

    authors: Łętowska M,Przybylska Z,Piotrowski D,Lachert E,Rosiek A,Rzymkiewicz L,Cardoso M

    更新日期:2016-03-01 00:00:00

  • Lung transplantation complicated by graft-versus-host disease and confounded by incidental transfusion-associated macrochimerism.

    abstract:BACKGROUND:Passenger lymphocyte-mediated graft-versus-host disease (GVHD) in solid organ transplantation (SOT-GVHD) is considered a rare complication, particularly among recipients of lung allografts. The risk of transfusion-associated GVHD (TA-GVHD) in solid organ transplants is also considered rare. The suspicion of ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2008.01835.x

    authors: Pavenski K,Kamel-Reid S,Wei C,Cserti-Gazdewich CM

    更新日期:2008-10-01 00:00:00

  • Incidence of breakage of human RBCs frozen with 40-percent wt/vol glycerol using two different methods for storage at -80 degrees C.

    abstract:BACKGROUND:We reported previously that the incidence of breakage was 34.2 percent when human RBCs were frozen with 40-percent wt/vol glycerol in polyolefin plastic bags stored in aluminum containers at -80 degrees C and subjected to transportation. When human RBCs were frozen with 40-percent wt/vol glycerol at -80 degr...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2003.00317.x

    authors: Valeri CR,Lane JP,Srey R,Ragno G

    更新日期:2003-03-01 00:00:00

  • Red blood cell product utilization in patients undergoing allogeneic stem cell transplantation.

    abstract:BACKGROUND:The risk of transfusion reactions (TR) and the cost of blood has led to efforts to reduce blood use. We changed our practice to transfuse just one instead of two units of red blood cells (RBC) when hemoglobin ≤8 g/dL due to patient blood management (PBM) recommendations. METHODS AND MATERIALS:We compared RB...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15285

    authors: Gastecki K,Shanley R,Welbig J,Cohn C,Brunstein CG

    更新日期:2019-07-01 00:00:00

  • Maternal human platelet antigen-1a antibody level correlates with the platelet count in the newborns: a retrospective study.

    abstract:BACKGROUND:Maternal plasma and/or serum levels of anti-HPA-1a at delivery were compared to neonatal platelet (PLT) counts. STUDY DESIGN AND METHODS:Samples from HPA-1bb women who gave birth to children with thrombocytopenia or had anamnestic information about a previous child with neonatal alloimmune thrombocytopenia ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01063.x

    authors: Killie MK,Husebekk A,Kaplan C,Taaning E,Skogen B

    更新日期:2007-01-01 00:00:00

  • Prospective-concurrent audits and medical consultation for platelet transfusions.

    abstract::Prospective-concurrent audits and medical consultation were associated with a 56 percent decrease in the use of platelet concentrates at a tertiary care medical center. This study documents that mandatory pretransfusion review of component requests can alter hospital transfusion practices. ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1987.27287150198.x

    authors: Simpson MB

    更新日期:1987-03-01 00:00:00

  • Safety of blood donation by individuals over age 70 and their contribution to the blood supply in five developed countries: a BEST Collaborative group study.

    abstract:BACKGROUND:Some countries impose an upper age limit on whole blood and double RBC donation while others do not. We evaluated the safety of blood donation in older individuals (≥71 years), and their contribution to the blood supply of five countries. STUDY DESIGN AND METHODS:Twelve blood center members of the Biomedica...

    journal_title:Transfusion

    pub_type: 杂志文章,多中心研究

    doi:10.1111/trf.15132

    authors: Goldman M,Germain M,Grégoire Y,Vassallo RR,Kamel H,Bravo M,Irving DO,Di Angelantonio E,Steele WR,O'Brien SF,Biomedical Excellence for Safer Transfusion Collaborative (BEST) Investigators.

    更新日期:2019-04-01 00:00:00

  • The role of inflammation in intravenous immune globulin-mediated hemolysis.

    abstract::Intravenous immune globulin (IVIG) therapy has shown great success in a number of autoimmune and inflammatory conditions and its use continues to increase worldwide. There is growing awareness of significant side effects of high-dose IVIG: however, particularly severe hemolysis in patients that are non-group O. It has...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.13097

    authors: Pendergrast J,Willie-Ramharack K,Sampson L,Laroche V,Branch DR

    更新日期:2015-07-01 00:00:00

  • A single-center prospective study on the safety of plasma exchange procedures using a double-viral-inactivated and prion-reduced solvent/detergent fresh-frozen plasma as the replacement fluid in the treatment of thrombotic microangiopathy.

    abstract:BACKGROUND:Patients presenting with acute episodes of thrombotic microangiopathies (TMAs) require urgent access to plasma exchange (PEX). OctaplasLG, a solvent/detergent fresh-frozen plasma product that has undergone viral inactivation and prion reduction step, has been used in our institution since 2013, replacing Oct...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1111/trf.13877

    authors: Vendramin C,McGuckin S,Alwan F,Westwood JP,Thomas M,Scully M

    更新日期:2017-01-01 00:00:00

  • Demographic changes and predicting blood supply and demand in the Netherlands.

    abstract:BACKGROUND:Concerns have been raised that aging of the general population will increase the demand for blood products. Modeling can be applied to assess trends in blood demand and supply and predict how these will develop over time. STUDY DESIGN AND METHODS:We developed mathematical models to describe and predict the ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02716.x

    authors: Borkent-Raven BA,Janssen MP,Van Der Poel CL

    更新日期:2010-11-01 00:00:00

  • Electronic enhancements to blood ordering reduce component waste.

    abstract:BACKGROUND:The effect on component wastage after enhancing the clinician's ability to order blood products in the computerized physician order entry (CPOE) system was investigated in a multihospital network. METHODS:A novel field was added to the CPOE of eight hospitals within a health care system allowing the physici...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13399

    authors: Yazer MH,Deandrade DS,Triulzi DJ,Wisniewski MK,Waters JH

    更新日期:2016-03-01 00:00:00

  • The impact of temporary deferral due to low hemoglobin: future return, time to return, and frequency of subsequent donation.

    abstract:BACKGROUND:This study investigated the effects of a 6-month deferral due to low hemoglobin (Hb) on the subsequent donation patterns of Australian whole blood donors. STUDY DESIGN AND METHODS:The study was a retrospective cohort study of the donation patterns of all whole blood donors deferred for low Hb during a 2-mon...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02881.x

    authors: Hillgrove T,Moore V,Doherty K,Ryan P

    更新日期:2011-03-01 00:00:00