Abstract:
:In the present work we developed a MPN quantitative real-time PCR (MPN-qPCR) method for a fast and reliable detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in minimally processed vegetables. In order to validate the proposed technique, the results were compared with conventional MPN followed by phenotypic and biochemical assays methods. When L. monocytogenes and E. coli O157:H7 were artificially inoculated in fresh-cut vegetables, a concentration as low as 1 CFU g(-1) could be detected in 48 hours for both pathogens. qPCR alone allowed a limit of detection of 10(1) CFU g(-1) after 2 hours of enrichment for L. monocytogenes and E. coli O157:H7. Since minimally processed ready-to-eat vegetables are characterized by very short shelf life, our method can potentially address the consistent reduction of time for microbial analysis, allowing a better management of quality control. Moreover, the occurrences of both pathogenic bacteria in mixed salad samples and fresh-cut melons were monitored in two production plants from the receipt of the raw materials to the early stages of shelf life. No sample was found to be contaminated by L. monocytogenes. One sample of raw mixed salad was found positive to an H7 enterohemorrhagic serotype.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Russo P,Botticella G,Capozzi V,Massa S,Spano G,Beneduce Ldoi
10.1155/2014/608296subject
Has Abstractpub_date
2014-01-01 00:00:00pages
608296eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
杂志文章abstract::Recent progress in microscopy technologies, biological markers, and automated processing methods is making possible the development of gene expression atlases at cellular-level resolution over whole embryos. Raw data on gene expression is usually very noisy. This noise comes from both experimental (technical/methodolo...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/986436
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
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更新日期:2019-03-21 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/3162649
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/786480
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pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2019/8575398
更新日期:2019-02-07 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究
doi:10.1155/2015/828120
更新日期:2015-01-01 00:00:00
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pub_type: 杂志文章,随机对照试验
doi:10.1155/2016/9190814
更新日期:2016-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/861460
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/403120
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
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doi:10.1155/2015/849571
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doi:10.1155/2018/1871598
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/2538765
更新日期:2018-01-29 00:00:00
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journal_title:BioMed research international
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doi:10.1155/2020/7926754
更新日期:2020-12-02 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/314543
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/6274545
更新日期:2019-04-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/256549
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4725141
更新日期:2020-08-18 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8465971
更新日期:2020-02-25 00:00:00