Abstract:
:Adenine nucleotides through P2Y1 receptor stimulation are known to control retinal progenitor cell (RPC) proliferation by modulating expression of the p57KIP2, a cell cycle regulator. However, the role of Gi protein-coupled P2Y12 and P2Y13 receptors also activated by adenine nucleotides in RPC proliferation is still unknown. Gene expression of the purinergic P2Y12 subtype was detected in rat retina during early postnatal days (P0 to P5), while expression levels of P2Y13 were low. Immunohistochemistry assays performed with rat retina on P3 revealed P2Y12 receptor expression in both Ki-67-positive cells in the neuroblastic layer and Ki-67-negative cells in the ganglion cell layer and inner nuclear layer. Nonetheless, P2Y13 receptor expression could not be detected in any stratum of rat retina. Intravitreal injection of PSB 0739 or clopidogrel, both selective P2Y12 receptor antagonists, increased by 20 and 15%, respectively, the number of Ki-67-positive cells following 24 h of exposure. Moreover, the P2Y12 receptor inhibition increased cyclin D1 and decreased p57KIP2 expression. However, there were no changes in the S phase of the cell cycle (BrdU-positive cells) or in mitosis (phospho-histone-H3-positive cells). Interestingly, an increase in the number of cyclin D1/TUNEL-positive cells after treatment with PSB 0739 was observed. These data suggest that activation of P2Y12 receptors is required for the successful exit of RPCs from cell cycle in the postnatal rat retina.
journal_name
Mol Neurobioljournal_title
Molecular neurobiologyauthors
de Almeida-Pereira L,Repossi MG,Magalhães CF,Azevedo RF,Corrêa-Velloso JDC,Ulrich H,Ventura ALM,Fragel-Madeira Ldoi
10.1007/s12035-018-1012-1subject
Has Abstractpub_date
2018-11-01 00:00:00pages
8612-8624issue
11eissn
0893-7648issn
1559-1182pii
10.1007/s12035-018-1012-1journal_volume
55pub_type
杂志文章abstract::For the epigenetic characterization of neuromyelitis optica (NMO), we determined whether messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) are expressed differentially in subjects with and without NMO. lncRNA and mRNA expression profiles of NMO patients and healthy controls were generated by using microarray an...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-9754-0
更新日期:2017-04-01 00:00:00
abstract::Military personnel are often subjected to sleep deprivation (SD) during combat operations. Since SD is a severe stress and alters neurochemical metabolism in the brain, a possibility exists that acute or long-term SD will influence blood-brain barrier (BBB) function and brain pathology. This hypothesis was examined in...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9236-9
更新日期:2015-10-01 00:00:00
abstract::The aim of this work was to study the effect of glucagon-like peptide-2 (GLP-2) on the cyclic guanosine monophosphate (cGMP) signalling pathway and whether insulin or epidermal growth factor (EGF) might modulate the effects of GLP-2. GLP-2 produced a dose-dependent decrease in intracellular sodium nitroprusside-induce...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-012-8298-1
更新日期:2012-10-01 00:00:00
abstract::Spinal cord injury (SCI) is a traumatic event resulting in disturbances to normal sensory, motor, or autonomic functions, which ultimately impacts a patient's physical, psychological, and social well-being. Until now, no available therapy for SCI can effectively slow down or halt the disease progression. Compared to t...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-015-9157-7
更新日期:2016-07-01 00:00:00
abstract::Cannabidiol (CBD), one of the most abundant Cannabis sativa-derived compounds, has been implicated with neuroprotective effect in several human pathologies. Until now, no undesired side effects have been associated with CBD. In this study, we evaluated CBD's neuroprotective effect in terminal differentiation (mature) ...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-014-8843-1
更新日期:2015-08-01 00:00:00
abstract::NMDA receptor signaling is critical for the development of synaptic plasticity, learning, and memory, and dysregulation of NMDAR signaling is implicated in a number of neurological disorders including schizophrenia (SZ). Previous work has demonstrated that the STriatal-Enriched protein tyrosine Phosphatase 61 kDa (STE...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-017-0555-x
更新日期:2018-04-01 00:00:00
abstract::Ascorbate, the reduced form of vitamin C, is highly concentrated in the central nervous system (CNS), including the retina, where it plays important physiological functions. In the CNS, the plasma membrane transporter sodium vitamin C co-transporter 2 (SVCT2) is responsible for ascorbate transport in neurons. The neur...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-0962-7
更新日期:2018-10-01 00:00:00
abstract::The signaling cascades triggered by neurotrophins such as BDNF and by several neurotransmitters and hormones lead to the rapid induction of gene transcription by increasing the intracellular concentration of cAMP and Ca2+. This review examines the mechanisms by which these second messengers control transcriptional ini...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1385/MN:30:1:091
更新日期:2004-08-01 00:00:00
abstract::Topoisomerase I (topo I) is a nuclear enzyme which participates in most DNA transactions. It was shown to be inhibited in depolarized neurons by poly adenosine diphosphate (ADP)-ribosylation of the enzyme protein. We demonstrated previously an age and sex dependent topo I activity and enzyme protein level in the vario...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-008-8044-x
更新日期:2008-12-01 00:00:00
abstract::The retina is an extension of the central nervous system and has been considered to be a simplified, more tractable and accessible version of the brain for a variety of neuroscience investigations. The optic nerve displays changes in response to underlying neurodegenerative diseases, such as stroke, multiple sclerosis...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-019-01863-8
更新日期:2020-04-01 00:00:00
abstract::Recent evidence suggests that hypoxia preconditioning can alter the microRNA (miRNA) profile of extracellular vesicles (EVs) and has better neuroprotective effects when enriched miRs are delivered to recipients. However, the roles of exosomal miRNAs in regulating ischaemia-reperfusion (IR)-induced pain hypersensitivit...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-020-02159-y
更新日期:2021-02-01 00:00:00
abstract::Sphingosine-1-phosphate (S1P) produced by sphingosine kinases (SPHK1 and SPHK2) is a signaling molecule involved in cell proliferation and formation of cellular junctions. In this study, we characterized the retinas of Sphk1 knockout (KO) mice by electron microscopy and immunocytochemistry. We also tested cultured Mül...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-019-1599-x
更新日期:2019-10-01 00:00:00
abstract::Multiple players are involved in motor and sensory dysfunctions after spinal cord injury (SCI). Therefore, therapeutic approaches targeting these various players in the damage cascade hold considerable promise for the treatment of traumatic spinal cord injury. Soluble epoxide hydrolase (sEH) is an endogenous key enzym...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9118-1
更新日期:2016-04-01 00:00:00
abstract::Protein aggregation is involved in a variety of diseases. Alteration of the aggregation pathway, either to produce less toxic structures or to increase aggregate clearance, is a promising therapeutic route. Both active and passive immunization has been used for this purpose. However, the mechanism of action of antibod...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9148-8
更新日期:2016-04-01 00:00:00
abstract::Rotenone, a pesticide, causes neurotoxicity via the mitochondrial complex-I inhibition. The present study was conducted to evaluate the role of endoplasmic reticulum (ER) stress in rotenone-induced neuronal death. Cell viability, cytotoxicity, reactive oxygen species (ROS) generation, nitrite level, mitochondrial memb...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-014-9001-5
更新日期:2016-01-01 00:00:00
abstract::For more than a century dendritic spines have been a source of fascination and speculation. The long-held belief that these anatomical structures are involved in learning and memory are addressed. Specifically, two lines of evidence that support this claim are reviewed. In the first, we review evidence that experiment...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1385/MN:29:2:117
更新日期:2004-04-01 00:00:00
abstract::Nicotine has a broad impact on both the central and peripheral nervous systems. Over the past decades, an increasing number of genes potentially involved in nicotine addiction have been identified by different technical approaches. However, the molecular mechanisms underlying nicotine addiction remain largely unknown....
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-014-8874-7
更新日期:2015-08-01 00:00:00
abstract::Aromatase inhibitors (AIs) have been commonly used as an effective adjuvant therapy in treatment of breast cancer, especially for menopausal women with estrogen receptor-positive breast cancer. Due to the nature of aromatase, the key enzyme for endogenous estrogen synthesis, inhibitory of aromatase-induced side effect...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-015-9341-9
更新日期:2016-08-01 00:00:00
abstract::We have recently demonstrated that δ-opioid receptor (DOR) activation attenuates α-synuclein expression/aggregation induced by MPP(+) and/or severe hypoxia. Since α-synuclein plays a critical role in the pathogenesis of Parkinson's disease, DOR activation may trigger an antiparkinson pathway(s) against α-synuclein-ind...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-018-1316-1
更新日期:2019-05-01 00:00:00
abstract::Astrocytes comprise the major cell type in the central nervous system (CNS) and they are essential for support of neuronal functions by providing nutrients and regulating cell-to-cell communication. Astrocytes also are immune-like cells that become reactive in response to neuronal injury. Phospholipases A2 (PLA2) are ...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1385/MN:31:1-3:027
更新日期:2005-01-01 00:00:00
abstract::Familial (FAD) and sporadic (SAD) Alzheimer's disease do not share all pathomechanisms, but knowledge on their molecular differences is limited. We previously reported that cell cycle control distinguishes lymphocytes from SAD and FAD patients. Significant differences were found in p21 levels of SAD compared to FAD ly...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-0105-y
更新日期:2017-09-01 00:00:00
abstract::Up to now, survivin has been recommended as a prognostic and diagnostic indicator in glioma patients. However, there are still many controversies. Here, a meta-analysis was conducted to draw a more definitive conclusion on the correlation of survivin with overall survival (OS), age, gender, and WHO grade. Eligible stu...
journal_title:Molecular neurobiology
pub_type: 杂志文章,meta分析,收录出版
doi:10.1007/s12035-014-8823-5
更新日期:2015-01-01 00:00:00
abstract::The global trend of the phenomenon of population ageing has dramatic consequences on public health and the incidence of neurodegenerative diseases. Physiological changes that occur during normal ageing of the brain may exacerbate and initiate pathological processes that may lead to neurodegenerative disorders, especia...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-012-8282-9
更新日期:2012-08-01 00:00:00
abstract::Reelin is an extracellular glycoprotein involved in key cellular processes in developing and adult nervous system, including regulation of neuronal migration, synapse formation, and plasticity. Most of these roles are mediated by the intracellular phosphorylation of disabled-1 (Dab1), an intracellular adaptor molecule...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-016-0177-8
更新日期:2017-10-01 00:00:00
abstract::The striatum is divided into two compartments, the striosomes and extrastriosomal matrix, which differ in several cytochemical markers, input-output connections, and time of neurogenesis. Since it is thought that limbic, reward-related information and executive aspects of behavioral information may be differentially p...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-008-8023-2
更新日期:2008-04-01 00:00:00
abstract::Lafora disease (LD, OMIM 254780, ORPHA501) is a fatal neurodegenerative disorder characterized by the presence of glycogen-like intracellular inclusions called Lafora bodies and caused, in the vast majority of cases, by mutations in either EPM2A or EPM2B genes, encoding respectively laforin and malin. In the last year...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-014-8747-0
更新日期:2015-01-01 00:00:00
abstract::The NMDA receptor, which is heavily involved in several human brain diseases, is a heteromeric ligand-gated ion channel that interacts with multiple intracellular proteins through the C-termini of different subunits. GluN2A and GluN2B are the two primary types of GluN2 subunits in the forebrain. During the development...
journal_title:Molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s12035-016-9715-7
更新日期:2017-03-01 00:00:00
abstract::Sonic hedgehog (Shh) has been found to regulate the angiogenic growth factor such as VEGF, Ang-1, and Ang-2 during ischemic insults, but the underlying mechanism is not fully understood. In this study, we employed oxygen-glucose deprivation (OGD) in astrocytes to mimic the ischemia in vitro. We found that OGD could in...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-013-8395-9
更新日期:2013-06-01 00:00:00
abstract::α-Synuclein (α-syn) has been recognized to induce neuroinflammation and to disturb nerve repair process in Parkinson's disease. However, the potential mechanisms underlying α-syn-induced impairment of adult neurogenesis remain unclear. In the present study, A53T mutant α--synuclein transgenic (A53Ttg/tg) mice, caspase...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9620-5
更新日期:2016-12-01 00:00:00
abstract::Parkinson's disease (PD) is characterized by dopaminergic (DA) neuron death in the substantia nigra (SN) and subsequent striatal adaptations. Mice treated with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrimidine (MPTP) are widely used as a model for PD. To assess the validity of the MPTP mouse model for PD p...
journal_title:Molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s12035-015-9103-8
更新日期:2016-04-01 00:00:00