Coexistence of two distinct Sulfurospirillum populations respiring tetrachloroethene-genomic and kinetic considerations.

Abstract:

:Two anaerobic bacterial consortia, each harboring a distinct Sulfurospirillum population, were derived from a 10 year old consortium, SL2, previously characterized for the stepwise dechlorination of tetrachloroethene (PCE) to cis-dichloroethene (cis-DCE) via accumulation of trichloroethene (TCE). Population SL2-1 dechlorinated PCE to TCE exclusively, while SL2-2 produced cis-DCE from PCE without substantial TCE accumulation. The reasons explaining the long-term coexistence of the populations were investigated. Genome sequencing revealed a novel Sulfurospirillum species, designated 'Candidatus Sulfurospirillum diekertiae', whose genome differed significantly from other Sulfurospirillum spp. (78%-83% ANI). Genome-wise, SL2-1 and SL2-2 populations are almost identical, but differences in their tetrachloroethene reductive dehalogenase sequences explain the distinct dechlorination patterns. An extended series of batch cultures were performed at PCE concentrations of 2-200 μM. A model was developed to determine their dechlorination kinetic parameters. The affinity constant and maximal growth rate differ between the populations: the affinity is 6- to 8-fold higher and the growth rate 5-fold lower for SL2-1 than SL2-2. Mixed cultivation of the enriched populations at 6 and 30 μM PCE showed that a low PCE concentration could be the driving force for both functional diversity of reductive dehalogenases and niche specialization of organohalide-respiring bacteria with overlapping substrate ranges.

journal_name

FEMS Microbiol Ecol

authors

Buttet GF,Murray AM,Goris T,Burion M,Jin B,Rolle M,Holliger C,Maillard J

doi

10.1093/femsec/fiy018

subject

Has Abstract

pub_date

2018-05-01 00:00:00

issue

5

eissn

0168-6496

issn

1574-6941

pii

4923013

journal_volume

94

pub_type

杂志文章
  • In vitro decomposition of Sphagnum by some microfungi resembles white rot of wood.

    abstract::The abilities of some ascomycetes (Myxotrichaceae) from a Sphagnum bog in Alberta to degrade cellulose, phenolics, and Sphagnum tissue were compared with those of two basidiomycetes. Most Myxotrichaceae degraded cellulose and tannic acid, and removed cell-wall components simultaneously from Sphagnum tissues, whereas t...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00071.x

    authors: Rice AV,Tsuneda A,Currah RS

    更新日期:2006-06-01 00:00:00

  • Phytate addition to soil induces changes in the abundance and expression of Bacillus β-propeller phytase genes in the rhizosphere.

    abstract::Phytate-mineralizing rhizobacteria (PMR) perform an essential function for the mineralization of organic phosphorus but little is known about their ecology in soils and rhizosphere. In this study, PCR-based methods were developed for detection and quantification of the Bacillus β-propeller phytase (BPP) gene. Experime...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2012.01480.x

    authors: Jorquera MA,Saavedra N,Maruyama F,Richardson AE,Crowley DE,del C Catrilaf R,Henriquez EJ,de la Luz Mora M

    更新日期:2013-02-01 00:00:00

  • The bottlenose dolphin (Tursiops truncatus) faecal microbiota.

    abstract::Cetaceans have evolved from herbivorous terrestrial artiodactyls closely related to ruminants and hippopotamuses. Delphinidae, a family included in this order, represent an extreme and successful re-adaptation of mammalian physiology to the marine habitat and piscivorous diet. The anatomical aspects of Delphinidae suc...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw055

    authors: Soverini M,Quercia S,Biancani B,Furlati S,Turroni S,Biagi E,Consolandi C,Peano C,Severgnini M,Rampelli S,Brigidi P,Candela M

    更新日期:2016-04-01 00:00:00

  • Multiple physiological states of a Pseudomonas fluorescens DR54 biocontrol inoculant monitored by a new flow cytometry protocol.

    abstract::A new fluorescence staining and flow cytometry protocol was developed to monitor several physiological states in biocontrol strain Pseudomonas fluorescens DR54 during storage survival in a stationary-phase culture, preparation of clay carrier for seed formulation, and establishment in a sugar beet spermosphere. The hi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00631.x

    authors: Nielsen TH,Sjøholm OR,Sørensen J

    更新日期:2009-03-01 00:00:00

  • The bacterial microbiota of Stolotermes ruficeps (Stolotermitidae), a phylogenetically basal termite endemic to New Zealand.

    abstract::Stolotermes ruficeps is a widespread, primitive, lower termite occupying dead and decaying wood of many tree species in New Zealand's temperate forests. We identified core bacterial taxa involved in gut processes through combined DNA- and RNA (cDNA)-based pyrosequencing analysis of the 16S nucleotide sequence from fiv...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12424

    authors: Reid NM,Addison SL,West MA,Lloyd-Jones G

    更新日期:2014-12-01 00:00:00

  • Beyond host regulation: Changes in gut microbiome of permissive and non-permissive hosts following parasitization by the wasp Cotesia flavipes.

    abstract::Koinobiont parasitoids regulate the physiology of their hosts, possibly interfering with the host gut microbiota and ultimately impacting parasitoid development. We used the parasitoid Cotesia flavipes to investigate if the regulation of the host would also affect the host gut microbiota. We also wondered if the effec...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz206

    authors: Cavichiolli de Oliveira N,Cônsoli FL

    更新日期:2020-02-01 00:00:00

  • Distinct composition signatures of archaeal and bacterial phylotypes in the Wanda Glacier forefield, Antarctic Peninsula.

    abstract::Several studies have shown that microbial communities in Antarctic environments are highly diverse. However, considering that the Antarctic Peninsula is among the regions with the fastest warming rates, and that regional climate change has been linked to an increase in the mean rate of glacier retreat, the microbial d...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiu005

    authors: Pessi IS,Osorio-Forero C,Gálvez EJ,Simões FL,Simões JC,Junca H,Macedo AJ

    更新日期:2015-01-01 00:00:00

  • Pezizomycotina dominates the fungal communities of South China Sea sponges Theonella swinhoei and Xestospongia testudinaria.

    abstract::Compared with the knowledge of sponge-associated bacterial diversity and ecological roles, the fungal diversity and ecological roles of sponges remain largely unknown. In this study, the fungal diversity and protein synthesis potential in two South China Sea sponges Theonella swinhoei and Xestospongia testudinaria wer...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12446

    authors: Jin L,Liu F,Sun W,Zhang F,Karuppiah V,Li Z

    更新日期:2014-12-01 00:00:00

  • Effects of selected root exudate components on soil bacterial communities.

    abstract::Low-molecular-weight organic compounds in root exudates play a key role in plant-microorganism interactions by influencing the structure and function of soil microbial communities. Model exudate solutions, based on organic acids (OAs) (quinic, lactic, maleic acids) and sugars (glucose, sucrose, fructose), previously i...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01150.x

    authors: Shi S,Richardson AE,O'Callaghan M,DeAngelis KM,Jones EE,Stewart A,Firestone MK,Condron LM

    更新日期:2011-09-01 00:00:00

  • Aspect has a greater impact on alpine soil bacterial community structure than elevation.

    abstract::Gradients in environmental conditions, including climate factors and resource availability, occur along mountain inclines, providing a 'natural laboratory' to explore their combined impacts on microbial distributions. Conflicting spatial patterns observed across elevation gradients in soil bacterial community structur...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw253

    authors: Wu J,Anderson BJ,Buckley HL,Lewis G,Lear G

    更新日期:2017-03-01 00:00:00

  • Structure and activity of the denitrifying community in a maize-cropped field fertilized with composted pig manure or ammonium nitrate.

    abstract::One alternative to mineral fertilization is to use organic fertilizers. Our aim was to compare the impacts of 7-year applications of composted pig manure and ammonium nitrate on the structure and activity of the denitrifying community. Mineralization and organization of N, denitrification rates and N2O/N2 ratio were a...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00064.x

    authors: Dambreville C,Hallet S,Nguyen C,Morvan T,Germon JC,Philippot L

    更新日期:2006-04-01 00:00:00

  • Bulk soil and maize rhizosphere resistance genes, mobile genetic elements and microbial communities are differently impacted by organic and inorganic fertilization.

    abstract::Organic soil fertilizers, such as livestock manure and biogas digestate, frequently contain bacteria carrying resistance genes (RGs) to antimicrobial substances and mobile genetic elements (MGEs). The effects of different fertilizers (inorganic, manure, digestate) on RG and MGE abundance and microbial community compos...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiy027

    authors: Wolters B,Jacquiod S,Sørensen SJ,Widyasari-Mehta A,Bech TB,Kreuzig R,Smalla K

    更新日期:2018-04-01 00:00:00

  • Food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization.

    abstract::A modified fluorescence in situ hybridization (FISH) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. Under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-Proteobacteria -- Aeromonas hydrophila and Pse...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.12.001

    authors: Jezbera J,Hornák K,Simek K

    更新日期:2005-05-01 00:00:00

  • Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils.

    abstract::The severity of environmental conditions at Earth's frigid zones present attractive opportunities for microbial biomining due to their heightened potential as reservoirs for novel secondary metabolites. Arid soil microbiomes within the Antarctic and Arctic circles are remarkably rich in Actinobacteria and Proteobacter...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz031

    authors: Benaud N,Zhang E,van Dorst J,Brown MV,Kalaitzis JA,Neilan BA,Ferrari BC

    更新日期:2019-04-01 00:00:00

  • Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatum truffle-ground.

    abstract::Truffles are hypogeous ectomycorrhizal fungi. They belong to the genus Tuber and are currently considered a hot spot in fungal biology due to their ecological and economic relevance. Among all the species, Tuber magnatum is the most appreciated because of its special taste and aroma. The aim of this work was to set up...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00783.x

    authors: Zampieri E,Murat C,Cagnasso M,Bonfante P,Mello A

    更新日期:2010-01-01 00:00:00

  • Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats.

    abstract::Two wild-type strains of Lactobacillus plantarum previously isolated from fermented dry sausages were analysed for their ability to transfer antibiotic resistance plasmids in the gastrointestinal tract. For this purpose, we used gnotobiotic rats as an in vivo model. Rats were initially inoculated with the recipient En...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00212.x

    authors: Jacobsen L,Wilcks A,Hammer K,Huys G,Gevers D,Andersen SR

    更新日期:2007-01-01 00:00:00

  • An improved electroelution method for separation of DNA from humic substances in marine sediment DNA extracts.

    abstract::We present a method for the rapid and simple extraction of DNA from marine sediments using electroelution. It effectively separates DNA from compounds, including humic substances, that interfere with subsequent DNA quantification and amplification. After extraction of the DNA from the sediment into an aqueous solution...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00684.x

    authors: Kallmeyer J,Smith DC

    更新日期:2009-07-01 00:00:00

  • Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH.

    abstract::We combined denaturing gradient gel electrophoresis (DGGE), catalysed reporter deposition-FISH (CARD-FISH) and clone libraries to investigate the seasonality of the bacterial assemblage composition in north-west Mediterranean coastal waters. DGGE analysis indicated that bacterial diversity changed gradually throughout...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00276.x

    authors: Alonso-Sáez L,Balagué V,Sà EL,Sánchez O,González JM,Pinhassi J,Massana R,Pernthaler J,Pedrós-Alió C,Gasol JM

    更新日期:2007-04-01 00:00:00

  • Spatial distribution of protists in the presence of macroaggregates in a marine system.

    abstract::The spatial distribution of marine protistan communities in the presence of organic macroaggregates, formed from natural seawater, was studied in several microcosm experiments. The presence of macroaggregates had two main effects. First, the size of the communities of bacteria, flagellates and ciliates increased, as t...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2000.tb00741.x

    authors: Artolozaga I I,Ayo B,Latatu A,Azúa I I,Unanue M,Iriberri J

    更新日期:2000-09-01 00:00:00

  • Mitochondrial sequences of Rhipicephalus and Coxiella endosymbiont reveal evidence of lineages co-cladogenesis.

    abstract::Rhipicephalus ticks are competent vectors of several pathogens, such as Spotted Fever Group Rickettsiae (SFGR) and many Babesia species. Within this genus, different R. sanguineus s.l. lineages show an unequal vector competence and resistance regarding some pathogenic strains. Current literature supports that tick end...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa072

    authors: Coimbra-Dores MJ,Jaarsma RI,Carmo AO,Maia-Silva M,Fonville M,da Costa DFF,Brandão RML,Azevedo F,Casero M,Oliveira AC,Afonso SMS,Sprong H,Rosa F,Dias D

    更新日期:2020-06-01 00:00:00

  • microeco: An R package for data mining in microbial community ecology.

    abstract::A large amount of sequencing data is produced in microbial community ecology studies using the high-throughput sequencing technique, especially amplicon-sequencing-based community data. After conducting the initial bioinformatic analysis of amplicon sequencing data, performing the subsequent statistics and data mining...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa255

    authors: Liu C,Cui Y,Li X,Yao M

    更新日期:2020-12-17 00:00:00

  • Microbial interactions affecting the natural transformation of Bacillus subtilis in a model aquatic ecosystem.

    abstract::The involvement of microbial interactions in natural transformation of bacteria was evaluated using an aquatic model system. For this purpose, the naturally transformable Bacillus subtilis was used as the model bacterium which was co-cultivated with the protist Tetrahymena thermophila (a consumer) and/or the photosynt...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00148-X

    authors: Matsui K,Ishii N,Kawabata Z

    更新日期:2003-08-01 00:00:00

  • Spatial distribution and genetic structure of Cenococcum geophilum in coastal pine forests in Japan.

    abstract::The asexual ectomycorrhizal fungus Cenococcum geophilum has a wide geographic range in diverse forest ecosystems. Although its genetic diversity has been documented at a stand or regional scale, knowledge of spatial genetic structure is limited. We studied the genetic diversity and spatial structure of C. geophilum in...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv108

    authors: Matsuda Y,Takeuchi K,Obase K,Ito S

    更新日期:2015-10-01 00:00:00

  • New insight into stratification of anaerobic methanotrophs in cold seep sediments.

    abstract::Methane seepages typically harbor communities of anaerobic methane oxidizers (ANME); however, knowledge about fine-scale vertical variation of ANME in response to geochemical gradients is limited. We investigated microbial communities in sediments below a white microbial mat in the G11 pockmark at Nyegga by 16S rRNA g...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01153.x

    authors: Roalkvam I,Jørgensen SL,Chen Y,Stokke R,Dahle H,Hocking WP,Lanzén A,Haflidason H,Steen IH

    更新日期:2011-11-01 00:00:00

  • Diversity of RuBisCO and ATP citrate lyase genes in soda lake sediments.

    abstract::Sediments from six soda lakes of the Kulunda Steppe (Altai, Russia) and from hypersaline alkaline lakes of Wadi Natrun (Egypt) were analyzed for the presence of cbb and aclB genes encoding key enzymes Ci assimilation (RuBisCO in Calvin-Benson and ATP citrate lyase in rTCA cycles, respectively). The cbbL gene (RuBisCO ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00996.x

    authors: Kovaleva OL,Tourova TP,Muyzer G,Kolganova TV,Sorokin DY

    更新日期:2011-01-01 00:00:00

  • The role of plant-microbiome interactions in weed establishment and control.

    abstract::The soil microbiome plays an important role in the establishment of weeds and invasive plants. They associate with microorganisms supporting their growth and health. Weed management strategies, like tillage and herbicide treatments, to control weeds generally alter soil structure going alongside with changes in the mi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw138

    authors: Trognitz F,Hackl E,Widhalm S,Sessitsch A

    更新日期:2016-10-01 00:00:00

  • Response of a kleptoplastidic foraminifer to heterotrophic starvation: photosynthesis and lipid droplet biogenesis.

    abstract::The aim of this work is to document the complex nutritional strategy developed by kleptoplastic intertidal foraminifera. We study the mixotrophic ability of a common intertidal foraminifer, Elphidium williamsoni, by (i) investigating the phylogenetic identity of the foraminiferal kleptoplasts, (ii) following their oxy...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz046

    authors: Jauffrais T,LeKieffre C,Schweizer M,Jesus B,Metzger E,Geslin E

    更新日期:2019-05-01 00:00:00

  • Microbial diversity in production waters of a low-temperature biodegraded oil reservoir.

    abstract::Microbial communities in two production waters of a low-temperature and low-salinity petroleum reservoir in Canada were examined using cultural and molecular approaches. The predominant cultivated microorganisms were homoacetogens but sulfate-reducers, acetoclastic methanogens and denitrifiers also gave significant co...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2005.05.007

    authors: Grabowski A,Nercessian O,Fayolle F,Blanchet D,Jeanthon C

    更新日期:2005-11-01 00:00:00

  • Rhizosphere persistence of three Pythium oligandrum strains in tomato soilless culture assessed by DNA macroarray and real-time PCR.

    abstract::In tomato soilless culture, plant-disease optimal control and growth promotion are achieved when the rhizosphere is heavily colonized by the biocontrol agent Pythium oligandrum. Discrepancies in performance are generally attributed to the poor persistence of P. oligandrum on roots. In this study, three selected strain...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2007.00348.x

    authors: Le Floch G,Tambong J,Vallance J,Tirilly Y,Lévesque A,Rey P

    更新日期:2007-08-01 00:00:00

  • Diversity and function of soil microbes on montane gradients: the state of knowledge in a changing world.

    abstract::Mountains have a long history in the study of diversity. Like macroscopic taxa, soil microbes are hypothesized to be strongly structured by montane gradients, and recently there has been important progress in understanding how microbes are shaped by these conditions. Here, we summarize this literature and synthesize p...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa122

    authors: Looby CI,Martin PH

    更新日期:2020-09-01 00:00:00