An improved electroelution method for separation of DNA from humic substances in marine sediment DNA extracts.

Abstract:

:We present a method for the rapid and simple extraction of DNA from marine sediments using electroelution. It effectively separates DNA from compounds, including humic substances, that interfere with subsequent DNA quantification and amplification. After extraction of the DNA from the sediment into an aqueous solution, the crude sample is encased in 2% agarose gel and exposed to an electrical current, which draws the DNA out of the gel into a centrifugal filter vial. After electroelution, the sample is centrifuged to remove contaminants

journal_name

FEMS Microbiol Ecol

authors

Kallmeyer J,Smith DC

doi

10.1111/j.1574-6941.2009.00684.x

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

125-31

issue

1

eissn

0168-6496

issn

1574-6941

pii

FEM684

journal_volume

69

pub_type

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