Measuring growth of a phenanthrene-degrading bacterial inoculum in soil with a quantitative competitive polymerase chain reaction method.

Abstract:

:We measured growth of a phenanthrene-degrading bacterium, Arthrobacter, strain RP17, in Forbes soil, amended with 500 µg g(-1) phenanthrene using a quantitative competitive polymerase chain reaction method. The inoculum, which was not indigenous to Forbes soil, grew from 5.55x10(5) colony forming units (cfu) g(-1) to 1.97x10(7) cfu g(-1) within 100 h after the cells were added to the soil. Maximum population density was reached before the highest degradation rate was observed 150 h after the cells were added to soil. Population density remained stable even after 56% of the phenanthrene had mineralized. This study is one of the few documented examples of growth by a non-indigenous bacterium in a non-sterile soil amended with a pollutant.

journal_name

FEMS Microbiol Ecol

authors

Schwartz E,V Trinh S,M Scow K

doi

10.1111/j.1574-6941.2000.tb00748.x

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

1-7

issue

1

eissn

0168-6496

issn

1574-6941

pii

S0168649600000660

journal_volume

34

pub_type

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