Abstract:
:Abstract Studies on the diversity of environmental culturable Pseudomonas populations are dependent on the isolation procedure. This procedure includes the use of selective media which may influence the recovery of strains and thus the diversity described. In this study, we assessed the use of two agar isolation media for describing the diversity of soil- and root-inhabiting Pseudomonas associated with the perennial grass Molinia coerulea. A total of 382 Pseudomonas strains were recovered on either non-selective Angle's medium, or on Gould's modified S1 (mS1) Pseudomonas-selective medium. Their diversity was assessed by restriction analysis of PCR (polymerase chain reaction)-amplified 16S-23S rDNA internal transcript spacer sequences. The comparison of mS1- and Angle-recovered populations showed that the use of mS1 selective medium led to an underestimation of both Pseudomonas counts and diversity, especially in the soil environment.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Tarnawski S,Hamelin J,Locatelli L,Aragno M,Fromin Ndoi
10.1016/S0168-6496(03)00130-2subject
Has Abstractpub_date
2003-07-01 00:00:00pages
97-104issue
2eissn
0168-6496issn
1574-6941pii
FEM97journal_volume
45pub_type
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