Abstract:
:Abstract In a previous study, we isolated a collection of atrazine-degrading bacteria from various soils. The aim of this study was to localise the atrazine-degrading genes in these 25 atrazine-degrading strains. In the case of the Gram-negative strains of Chelatobacter heintzii, six to seven plasmids were observed. The atzABC and trzD genes were located on two or three plasmids with variable molecular masses. For the Gram-positive strains of Arthrobacter crystallopoietes, the atzBC genes were located on a single plasmid of 117 kb. The organisation of atrazine-degrading genes seems to be highly variable between the strains studied. We have shown by a specific PCR the occurrence of IS1071-like sequences in all strains carrying atzBC genes, while these sequences seem to be absent in strains carrying only the atzA gene. Thus, we show a strong correlation between the presence of these insertion sequences and the presence of some genes involved in atrazine degradation.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Rousseaux S,Soulas G,Hartmann Adoi
10.1111/j.1574-6941.2002.tb00967.xsubject
Has Abstractpub_date
2002-07-01 00:00:00pages
69-75issue
1eissn
0168-6496issn
1574-6941pii
FEM69journal_volume
41pub_type
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