Plasmid localisation of atrazine-degrading genes in newly described Chelatobacter and Arthrobacter strains.

Abstract:

:Abstract In a previous study, we isolated a collection of atrazine-degrading bacteria from various soils. The aim of this study was to localise the atrazine-degrading genes in these 25 atrazine-degrading strains. In the case of the Gram-negative strains of Chelatobacter heintzii, six to seven plasmids were observed. The atzABC and trzD genes were located on two or three plasmids with variable molecular masses. For the Gram-positive strains of Arthrobacter crystallopoietes, the atzBC genes were located on a single plasmid of 117 kb. The organisation of atrazine-degrading genes seems to be highly variable between the strains studied. We have shown by a specific PCR the occurrence of IS1071-like sequences in all strains carrying atzBC genes, while these sequences seem to be absent in strains carrying only the atzA gene. Thus, we show a strong correlation between the presence of these insertion sequences and the presence of some genes involved in atrazine degradation.

journal_name

FEMS Microbiol Ecol

authors

Rousseaux S,Soulas G,Hartmann A

doi

10.1111/j.1574-6941.2002.tb00967.x

subject

Has Abstract

pub_date

2002-07-01 00:00:00

pages

69-75

issue

1

eissn

0168-6496

issn

1574-6941

pii

FEM69

journal_volume

41

pub_type

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