Impact of streambed morphology on the abundance and activity of ammonia-oxidizing bacteria.

Abstract:

:Ammonia oxidizers catalyze the first step of nitrification. Combined microbial nitrification-denitrification activities are essential for the removal of excess nitrogen from water bodies. In sandy streambeds, bed form structures are created by water flow and lead to the creation of heterogeneous microenvironments. The objective of this study, therefore, was to investigate the effect of bed form morphology on the abundance and activity of ammonia-oxidizing bacteria (AOB) within a benthic biofilm. An 8-month-old benthic biofilm was established in a recirculating laboratory flume under controlled flow conditions and frequent amendment with ammonium. The sand bed was arranged into bed form structures. The highest concentrations of chlorophyll a (indicative of algae) were measured on the upstream side of the bed forms. The biofilm was dominated by Nitrosospira species, and amoA gene abundance was higher on the downstream sides of the bed forms with no significant difference in oxygen consumption between the upstream and downstream sections of the bed form. In contrast, potential ammonium oxidation rates were higher on the upstream sides of the bed forms. The results suggest that bed form morphology can affect the spatial distribution and activity of AOB, possibly through the creation of distinct microhabitats. These results contribute to our understanding of nitrogen transformations and removal from streams.

journal_name

FEMS Microbiol Ecol

authors

Yanuka-Golub K,Arnon S,Nejidat A

doi

10.1111/1574-6941.12385

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

175-83

issue

1

eissn

0168-6496

issn

1574-6941

journal_volume

90

pub_type

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