Abstract:
:Sulfate-reducing bacteria (SRB) pose a serious problem to offshore oil industries by producing sulfide, which is highly reactive, corrosive and toxic. The dissimilatory sulfite reductase (dsr) gene encodes for enzyme dissimilatory sulfite reductase and catalyzes the conversion of sulfite to sulfide. Because this gene is required by all sulfate reducers, it is a potential candidate as a functional marker. Denaturing gradient gel electrophoresis fingerprints revealed the presence of considerable genetic diversity in the DNA extracts achieved from production water collected from various oil fields. A quantitative PCR (qPCR) assay was developed for rapid and accurate detection of dsrB in oil field samples. A standard curve was prepared based on a plasmid containing the appropriate dsrB fragment from Desulfomicrobium norvegicum. The quantification range of this assay was six orders of magnitude, from 4.5 x 10(7) to 4.5 x 10(2) copies per reaction. The assay was not influenced by the presence of foreign DNA. This assay was tested against several DNA samples isolated from formation water samples collected from geographically diverse locations of India. The results indicate that this qPCR approach can provide valuable information related to the abundance of the bisulfite reductase gene in harsh environmental samples.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Agrawal A,Lal Bdoi
10.1111/j.1574-6941.2009.00714.xsubject
Has Abstractpub_date
2009-08-01 00:00:00pages
301-12issue
2eissn
0168-6496issn
1574-6941pii
FEM714journal_volume
69pub_type
杂志文章abstract::Tedania sp. is a dominant sponge that is ubiquitous along the southeast coast of China. High-throughput sequencing and transmission electron microscopy were used to describe a detailed profile of sponge-associated microbiomes at seven life stages: adult, embryo-containing spawning adult, embryo, pre-competent larva at...
journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
pub_type: 杂志文章,评审
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journal_title:FEMS microbiology ecology
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pub_type: 共识发展会议,杂志文章
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journal_title:FEMS microbiology ecology
pub_type: 杂志文章
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journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
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journal_title:FEMS microbiology ecology
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