Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods.


:Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 x 10(7) cm(-3), representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the alpha-, beta-, gamma-, and delta-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community.


FEMS Microbiol Ecol


Süss J,Engelen B,Cypionka H,Sass H




Has Abstract


2004-12-27 00:00:00














  • Subterranean microbial populations metabolize hydrogen and acetate under in situ conditions in granitic groundwater at 450 m depth in the Äspö Hard Rock Laboratory, Sweden.

    abstract::Pressure-resistant circulating systems were constructed to enable the investigation of attached and unattached microbial populations under in situ pressure (2.5 MPa), diversity, dissolved gas and chemistry conditions. Three parallel flow cell cabinets were configured to allow observation of the effect on microbial met...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Pedersen K

    更新日期:2012-07-01 00:00:00

  • Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.

    abstract::Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Jourand P,Ducousso M,Loulergue-Majorel C,Hannibal L,Santoni S,Prin Y,Lebrun M

    更新日期:2010-05-01 00:00:00

  • Two dnaB genes are associated with the origin of replication of pQBR55, an exogenously isolated plasmid from the rhizosphere of sugar beet.

    abstract::Plasmid pQBR55 ( approximately 149 kb) represents one of five (Groups I-V) genetically distinct transfer proficient, mercury resistance plasmid groups that have been observed in the phytosphere pseudomonad community at a single geographic location in Oxford, UK. A 4.9-kb HindIII fragment was cloned from pQBR55 (a Grou...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Turner SL,Lilley AK,Bailey MJ

    更新日期:2002-11-01 00:00:00

  • Mitochondrial sequences of Rhipicephalus and Coxiella endosymbiont reveal evidence of lineages co-cladogenesis.

    abstract::Rhipicephalus ticks are competent vectors of several pathogens, such as Spotted Fever Group Rickettsiae (SFGR) and many Babesia species. Within this genus, different R. sanguineus s.l. lineages show an unequal vector competence and resistance regarding some pathogenic strains. Current literature supports that tick end...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Coimbra-Dores MJ,Jaarsma RI,Carmo AO,Maia-Silva M,Fonville M,da Costa DFF,Brandão RML,Azevedo F,Casero M,Oliveira AC,Afonso SMS,Sprong H,Rosa F,Dias D

    更新日期:2020-06-01 00:00:00

  • A bacterial culture-independent method to investigate chemically mediated control of bacterial epibiosis in marine invertebrates by using TRFLP analysis and natural bacterial populations.

    abstract::It has been postulated that a variety of physically undefended marine invertebrates have evolved strategies to control microbial epibiosis chemically. Ecologically meaningful experiments that demonstrate chemically mediated antibacterial effects are difficult due to the small number of cultivable bacteria. Based on th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Harder T,Lau SC,Tam WY,Qian PY

    更新日期:2004-01-01 00:00:00

  • Characterization of nifH gene expression, modification and rearrangement in Nodularia spumigena strain AV1.

    abstract::The annually reoccurring blooms that characterize the surface waters of the Baltic Sea are dominated by filamentous, heterocystous cyanobacteria such as Nodularia spumigena. In a previous study, we have demonstrated that N. spumigena strain AV1 differentiates heterocysts in the absence of detectable nitrogen fixation ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Vintila S,Selao T,Norén A,Bergman B,El-Shehawy R

    更新日期:2011-08-01 00:00:00

  • Stable symbionts across the HMA-LMA dichotomy: low seasonal and interannual variation in sponge-associated bacteria from taxonomically diverse hosts.

    abstract::Marine sponges host bacterial communities with important ecological and economic roles in nature and society, yet these benefits depend largely on the stability of host-symbiont interactions and their susceptibility to changing environmental conditions. Here, we investigated the temporal stability of complex host-micr...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Erwin PM,Coma R,López-Sendino P,Serrano E,Ribes M

    更新日期:2015-10-01 00:00:00

  • Spatial heterogeneity of eukaryotic microbial communities in an unstudied geothermal diatomaceous biological soil crust: Yellowstone National Park, WY, USA.

    abstract::Knowledge of microbial communities and their inherent heterogeneity has dramatically increased with the widespread use of high-throughput sequencing technologies, and we are learning more about the ecological processes that structure microbial communities across a wide range of environments, as well as the relative sc...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Meadow JF,Zabinski CA

    更新日期:2012-10-01 00:00:00

  • Genetic diversity of Mimosa pudica rhizobial symbionts in soils of French Guiana: investigating the origin and diversity of Burkholderia phymatum and other beta-rhizobia.

    abstract::The genetic diversity of 221 Mimosa pudica bacterial symbionts trapped from eight soils from diverse environments in French Guiana was assessed by 16S rRNA PCR-RFLP, REP-PCR fingerprints, as well as by phylogenies of their 16S rRNA and recA housekeeping genes, and by their nifH, nodA and nodC symbiotic genes. Interest...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Mishra RP,Tisseyre P,Melkonian R,Chaintreuil C,Miché L,Klonowska A,Gonzalez S,Bena G,Laguerre G,Moulin L

    更新日期:2012-02-01 00:00:00

  • Activity, structure and dynamics of the methanogenic archaeal community in a flooded Italian rice field.

    abstract::Methane production was studied in an Italian rice field over two consecutive years (1998, 1999) by measuring the rates of total and acetate-dependent methanogenesis in soil and root samples. Population dynamics of methanogens were followed by terminal restriction fragment length polymorphism and real-time PCR targetin...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Krüger M,Frenzel P,Kemnitz D,Conrad R

    更新日期:2005-02-01 00:00:00

  • Marine bacterial organisation around point-like sources of amino acids.

    abstract::Abstract To better understand the trigger for and use of motility in marine bacterial chemotaxis, specific (amino acids) chemical stimuli were used to test the effect on bacterial speed and reorientation. An assay system was developed to analyse bacterial behavioural responses to point-like nutrient sources (beads 10-...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Barbara GM,Mitchell JG

    更新日期:2003-02-01 00:00:00

  • Bacterial motility: links to the environment and a driving force for microbial physics.

    abstract::Bacterial motility was recognized 300 years ago. Throughout this history, research into motility has led to advances in microbiology and physics. Thirty years ago, this union helped to make run and tumble chemotaxis the paradigm for bacterial movement. This review highlights how this paradigm has expanded and changed,...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Mitchell JG,Kogure K

    更新日期:2006-01-01 00:00:00

  • Analysis of DGGE profiles to explore the relationship between prokaryotic community composition and biogeochemical processes in deep subseafloor sediments from the Peru Margin.

    abstract::The aim of this work was to relate depth profiles of prokaryotic community composition with geochemical processes in the deep subseafloor biosphere at two shallow-water sites on the Peru Margin in the Pacific Ocean (ODP Leg 201, sites 1228 and 1229). Principal component analysis of denaturing gradient gel electrophore...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Fry JC,Webster G,Cragg BA,Weightman AJ,Parkes RJ

    更新日期:2006-10-01 00:00:00

  • Diversity of methicillin-resistant staphylococci among wild Lepus granatensis: first detection of mecA-MRSA in hares.

    abstract::MRSA in humans, pets and livestock have been widely investigated, nevertheless, there is still little information of MRSA in wild animals. Therefore, this study aimed to investigate the occurrence and antimicrobial resistance profiles of methicillin-resistant staphylococci (MRS) in wild Iberian hares and to characteri...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Silva V,Pereira JE,Maltez L,Ferreira E,Manageiro V,Caniça M,Capelo JL,Igrejas G,Poeta P

    更新日期:2020-01-01 00:00:00

  • Inferring community dynamics of organohalide-respiring bacteria in chemostats by covariance of rdhA gene abundance.

    abstract::We have developed a novel approach to identifying and quantifying closely related organohalide-respiring bacteria. Our approach made use of the unique genomic associations of specific reductive dehalogenase subunit A encoding genes (rdhA) that exist in known strains of Dehalococcoides mccartyi and Desulfitobacterium a...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Marshall IP,Azizian MF,Semprini L,Spormann AM

    更新日期:2014-02-01 00:00:00

  • Identification of phenol- and p-cresol-producing intestinal bacteria by using media supplemented with tyrosine and its metabolites.

    abstract::To identify intestinal bacteria that produce phenols (phenol and p-cresol), we screened 153 strains within 152 species in 44 genera by culture-based assay using broth media supplemented with 200 µM each of tyrosine and its predicted microbial metabolic intermediates (4-hydroxyphenylpyruvate, DL-4-hydroxyphenyllactate,...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Saito Y,Sato T,Nomoto K,Tsuji H

    更新日期:2018-09-01 00:00:00

  • Methods for field measurement of antibiotic concentrations: limitations and outlook.

    abstract::The growing prevalence of antibiotic resistance poses an increasingly serious threat to human health. Although an important driver of antibiotic resistance is the continuous exposure of bacteria to sublethal concentrations of antibiotics in natural environments, antibiotic pollutants are not currently tracked globally...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Parthasarathy R,Monette CE,Bracero S,S Saha M

    更新日期:2018-08-01 00:00:00

  • Depth distribution of active bacteria and bacterial activity in lake sediment.

    abstract::Abstract The bacterial activity in sediments is often low considering the generally high bacterial abundance. Still, a large fraction of bacteria have been found active even in deep sediments. These findings suggest that sediment bacteria have comparatively low cell-specific production. We studied bacterial activity a...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Haglund AL,Lantz P,Törnblom E,Tranvik L

    更新日期:2003-10-01 00:00:00

  • The vertical distribution of tetA and intI1 in a deep lake is rather due to sedimentation than to resuspension.

    abstract::Lakes are exposed to anthropogenic pollution including the release of allochthonous bacteria into their waters. Antibiotic resistance genes (ARGs) stabilize in bacterial communities of temperate lakes, and these environments act as long-term reservoirs of ARGs. Still, it is not clear if the stabilization of the ARGs i...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Di Cesare A,Eckert EM,Cottin C,Bouchez A,Callieri C,Cortesini M,Lami A,Corno G

    更新日期:2020-02-01 00:00:00

  • Microbial dynamics and flagellate grazing during transition to winter in Lakes Hoare and Bonney, Antarctica.

    abstract::The planktonic microbial communities of Lakes Hoare and Bonney were investigated during transition into winter. We hypothesized that the onset of darkness induces changes in the functional role of autotrophic and heterotrophic microplankton. Bacteria decreased in Lake Hoare during March-April, while in Lake Bonney bac...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Thurman J,Parry J,Hill PJ,Priscu JC,Vick TJ,Chiuchiolo A,Laybourn-Parry J

    更新日期:2012-11-01 00:00:00

  • The structure and stability of the bacterioplankton community in Antarctic freshwater lakes, subject to extremely rapid environmental change.

    abstract::In this study, variation in the bacterioplankton community structure of three Antarctic lakes of different nutrient status, was determined in relation to physical and chemical gradients at depth and at time intervals, across the seasonal transition from winter ice-cover to the summer ice-free period. The three lakes s...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Pearce DA

    更新日期:2005-06-01 00:00:00

  • Strong linkage between plant and soil fungal communities along a successional coastal dune system.

    abstract::Complex interactions between plants and soil microorganisms drive key ecosystem and community properties such as productivity and diversity. In nutrient-poor systems such as sand dunes, plant traits and fungal symbioses related to nutrient acquisition can strongly influence vegetation dynamics. We investigated plant a...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Roy-Bolduc A,Laliberté E,Boudreau S,Hijri M

    更新日期:2016-10-01 00:00:00

  • Characterization of bacterial diversity to a depth of 1500 m in the Outokumpu deep borehole, Fennoscandian Shield.

    abstract::This paper demonstrates the first microbiological sampling of the Outokumpu deep borehole (2516 m deep) aiming at characterizing the bacterial community composition and diversity of sulphate-reducing bacteria (SRB) in Finnish crystalline bedrock aquifers. Sampling was performed using a 1500-m-long pressure-tight tube ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Itävaara M,Nyyssönen M,Kapanen A,Nousiainen A,Ahonen L,Kukkonen I

    更新日期:2011-08-01 00:00:00

  • Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis.

    abstract::Using different techniques of molecular biology we investigated the bacterial diversity of the chemocline of the meromictic Lake Cadagno. Cloning of a total community 16S rDNA PCR product and subsequent screening with a combination of amplified ribosomal DNA restriction analysis and temporal temperature gradient gel e...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Bosshard PP,Santini Y,Grüter D,Stettler R,Bachofen R

    更新日期:2000-02-01 00:00:00

  • Changes in fibre-adherent and fluid-associated microbial communities and fermentation profiles in the rumen of cattle fed diets differing in hay quality and concentrate amount.

    abstract::The rumen microbiota enable important metabolic functions to the host cattle. Feeding of starch-rich concentrate feedstuffs to cattle has been demonstrated to increase the risk of metabolic disorders and to significantly alter the rumen microbiome. Thus, alternative feeding strategies like the use of high-quality hay,...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Klevenhusen F,Petri RM,Kleefisch MT,Khiaosa-Ard R,Metzler-Zebeli BU,Zebeli Q

    更新日期:2017-09-01 00:00:00

  • Survey of selected antibiotic resistance genes in agricultural and non-agricultural soils in south-central Idaho.

    abstract::Improving our understanding of antibiotic resistance in soils is important for the protection of human, animal and ecological health. In south-central Idaho, antibiotic resistance genes (ARGs) [blaCTX-M-1, erm(B), sul1, tet(B), tet(M) and tet(X)] and a class 1 integron-integrase gene (intI1) were quantified in agricul...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Dungan RS,Strausbaugh CA,Leytem AB

    更新日期:2019-06-01 00:00:00

  • Extremotolerance in fungi: evolution on the edge.

    abstract::Our planet offers many opportunities for life on the edge: high and low temperatures, high salt concentrations, acidic and basic conditions and toxic environments, to name but a few extremes. Recent studies have revealed the diversity of fungi that can occur in stressful environments that are hostile to most eukaryote...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Gostincar C,Grube M,de Hoog S,Zalar P,Gunde-Cimerman N

    更新日期:2010-01-01 00:00:00

  • Sheep-urine-induced changes in soil microbial community structure.

    abstract::Soil microbial communities play an important role in nutrient cycling and nutrient availability, especially in unimproved soils. In grazed pastures, sheep urine causes local changes in nutrient concentration which may be a source of heterogeneity in microbial community structure. In the present study, we investigated ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Nunan N,Singh B,Reid E,Ord B,Papert A,Squires J,Prosser JI,Wheatley RE,McNicol J,Millard P

    更新日期:2006-05-01 00:00:00

  • Distinct bacterial assemblages reside at different depths in Arctic multiyear sea ice.

    abstract::Bacterial communities in Arctic sea ice play an important role in the regulation of nutrient and energy dynamics in the Arctic Ocean. Sea ice has vertical gradients in temperature, brine salinity and volume, and light and UV levels. Multiyear ice (MYI) has at least two distinct ice layers: old fresh ice with limited p...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Hatam I,Charchuk R,Lange B,Beckers J,Haas C,Lanoil B

    更新日期:2014-10-01 00:00:00

  • Mechanisms and recent advances in biological control mediated through the potato rhizosphere.

    abstract::Potato cultivation has a strategic role as a food source for the human population. Its promising future development relies on improving the control of the numerous microbial diseases that affect its growth. Numerous and recent studies on the potato rhizosphere, mycorrhizosphere and endorhiza reveal the presence of a d...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Diallo S,Crépin A,Barbey C,Orange N,Burini JF,Latour X

    更新日期:2011-03-01 00:00:00