Two dnaB genes are associated with the origin of replication of pQBR55, an exogenously isolated plasmid from the rhizosphere of sugar beet.

Abstract:

:Plasmid pQBR55 ( approximately 149 kb) represents one of five (Groups I-V) genetically distinct transfer proficient, mercury resistance plasmid groups that have been observed in the phytosphere pseudomonad community at a single geographic location in Oxford, UK. A 4.9-kb HindIII fragment was cloned from pQBR55 (a Group III plasmid) that facilitates autonomous replication of a narrow host range cloning vector, pKIL29, in the non-permissive host Pseudomonas putida UWC1. Sequencing revealed that the fragment contains a unique tandem array of dnaB genes (one partial and one complete), a homologue of the traA gene of plasmid RP4 and several potential open reading frames with little homology to any known sequences. The fragment also contains: a short region of direct and indirect repeats, two sequences that resemble the consensus Escherichia coli DnaA box motif, an A+T rich region and evidence of a GC-skew inversion. All features associated with origins of replication. Two specific oligonucleotide primer pairs, one targeted at the tandem dnaB genes and the other at the dnaB-traA arrangement were used for PCR analysis of other plasmids isolated from the same field site. PCR generated amplification products were only amplified from Group III plasmids (defined by RFLP typing) but not from plasmids belonging to Group I, II, IV or V. The nucleotide sequences of the amplified fragments were identical to those of pQBR55, even though the other Group III plasmids had distinct RFLP patterns. The results of a more general PCR screen, using primers to amplify a 389-bp fragment of all described pseudomonad dnaB genes, suggests that closely related dnaB sequences are only associated with Group III plasmids.

journal_name

FEMS Microbiol Ecol

authors

Turner SL,Lilley AK,Bailey MJ

doi

10.1111/j.1574-6941.2002.tb01010.x

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

209-15

issue

2

eissn

0168-6496

issn

1574-6941

pii

FEM209

journal_volume

42

pub_type

杂志文章
  • A bacterial culture-independent method to investigate chemically mediated control of bacterial epibiosis in marine invertebrates by using TRFLP analysis and natural bacterial populations.

    abstract::It has been postulated that a variety of physically undefended marine invertebrates have evolved strategies to control microbial epibiosis chemically. Ecologically meaningful experiments that demonstrate chemically mediated antibacterial effects are difficult due to the small number of cultivable bacteria. Based on th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00246-0

    authors: Harder T,Lau SC,Tam WY,Qian PY

    更新日期:2004-01-01 00:00:00

  • Impact of environmental factors on couplings between bacterial community composition and ectoenzymatic activities in a lacustrine ecosystem.

    abstract::This study examined the effects of temporal changes in bacterial community composition (BCC) and environmental factors on potential ectoenzymatic activities (alpha-glucosidase, beta-glucosidase, alkaline phosphatase and leucine aminopeptidase) in a lacustrine ecosystem (Sep reservoir, France). BCC was assessed by term...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00730.x

    authors: Boucher D,Debroas D

    更新日期:2009-10-01 00:00:00

  • Compartmentalization of gypsum and halite associated with cyanobacteria in saline soil crusts.

    abstract::The interface between biological and geochemical components in the surface crust of a saline soil was investigated using X-ray diffraction, and variable pressure scanning electron microscopy in combination with energy dispersive X-ray spectrometry. Mineral compounds such as halite and gypsum were identified crystalliz...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw080

    authors: Canfora L,Vendramin E,Vittori Antisari L,Lo Papa G,Dazzi C,Benedetti A,Iavazzo P,Adamo P,Jungblut AD,Pinzari F

    更新日期:2016-06-01 00:00:00

  • Soil fungal community composition and functional similarity shift across distinct climatic conditions.

    abstract::A large part of ecosystem function in woodland systems depends on soil fungal communities. However, global climate change has the potential to fundamentally alter these communities as fungal species are filtered with changing environmental conditions. In this study, we examined the potential effects of climate on host...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa193

    authors: Bui A,Orr D,Lepori-Bui M,Konicek K,Young HS,Moeller HV

    更新日期:2020-12-02 00:00:00

  • The vertical distribution of bacterial and archaeal communities in the water and sediment of Lake Taihu.

    abstract::This study was conducted to characterize the vertical distribution of bacterial and archaeal communities in the water and sediment of Lake Taihu, which underwent a change in trophic status from oligotrophic to hypertrophic in last half of the 20th century. The results revealed that the bacterial communities in differe...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00761.x

    authors: Ye W,Liu X,Lin S,Tan J,Pan J,Li D,Yang H

    更新日期:2009-11-01 00:00:00

  • Effects of host traits and land-use changes on the gut microbiota of the Namibian black-backed jackal (Canis mesomelas).

    abstract::Host traits and environmental factors drive the natural variation in gut microbiota, and disruption in homeostasis can cause infections and chronic diseases. African wildlife is increasingly facing human-induced agricultural habitats, which also amplifies the contact probability with livestock with unknown consequence...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix123

    authors: Menke S,Meier M,Mfune JKE,Melzheimer J,Wachter B,Sommer S

    更新日期:2017-11-01 00:00:00

  • Thermophilic methanotrophs: in hot pursuit.

    abstract::Methane is a potent greenhouse gas responsible for 20-30% of global climate change effects. The global methane budget is ∼500-600 Tg y-1, with the majority of methane produced via microbial processes, including anthropogenic-mediated sources such as ruminant animals, rice fields, sewage treatment facilities and landfi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审

    doi:10.1093/femsec/fiz125

    authors: Houghton KM,Carere CR,Stott MB,McDonald IR

    更新日期:2019-09-01 00:00:00

  • A cold-loving crenarchaeon is a substantial part of a novel microbial community in cold sulphidic marsh water.

    abstract::In this paper, we report the identification and first characterization of a novel, cold-loving, prokaryotic community thriving among white-greenish 'streamers' in the cold (c. 10 degrees C) sulphurous water of the marsh Sippenauer Moor near Regensburg, Bavaria, Germany. It consists of the bacterial genus Thiothrix, th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00088.x

    authors: Koch M,Rudolph C,Moissl C,Huber R

    更新日期:2006-07-01 00:00:00

  • A dietary polyunsaturated fatty acid improves consumer performance during challenge with an opportunistic bacterial pathogen.

    abstract::A dietary deficiency in polyunsaturated fatty acids (PUFAs) and/or sterols can severely constrain growth and reproduction of invertebrate consumers. Single nutrients are potentially assigned to different physiological processes, for example to support defence mechanisms; therefore, lipid requirements of healthy and pa...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12407

    authors: Schlotz N,Pester M,Freese HM,Martin-Creuzburg D

    更新日期:2014-11-01 00:00:00

  • Coexistence of two distinct Sulfurospirillum populations respiring tetrachloroethene-genomic and kinetic considerations.

    abstract::Two anaerobic bacterial consortia, each harboring a distinct Sulfurospirillum population, were derived from a 10 year old consortium, SL2, previously characterized for the stepwise dechlorination of tetrachloroethene (PCE) to cis-dichloroethene (cis-DCE) via accumulation of trichloroethene (TCE). Population SL2-1 dech...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiy018

    authors: Buttet GF,Murray AM,Goris T,Burion M,Jin B,Rolle M,Holliger C,Maillard J

    更新日期:2018-05-01 00:00:00

  • Different diversity-functioning relationship in lake and stream bacterial communities.

    abstract::Biodiversity patterns have been successfully linked to many ecosystem functions, and microbial communities have been suspected to harbour a large amount of functionally redundant taxa. We manipulated the diversity of stream and lake water column bacterial communities and investigated how the reduction in diversity aff...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12101

    authors: Ylla I,Peter H,Romaní AM,Tranvik LJ

    更新日期:2013-07-01 00:00:00

  • Photosynthetic capacity of the endosymbiotic dinoflagellate Cladocopium sp. is preserved during digestion of its jellyfish host Mastigias papua by the anemone Entacmaea medusivora.

    abstract::The sea anemone Entacmaea medusivora (Actiniaria, Anthozoa) commonly feeds on the golden jellyfish Mastigias papua (Rhizostomeae, Scyphozoa) which harbours an endosymbiotic dinoflagellate of the genus Cladocopium (Symbiodiniaceae). In this study, we monitored the photosynthetic activity of the endosymbiotic microalgae...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz141

    authors: Vega de Luna F,Dang KV,Cardol M,Roberty S,Cardol P

    更新日期:2019-10-01 00:00:00

  • Characterisation of the culturable heterotrophic bacterial community in a small eutrophic lake (Priest Pot).

    abstract::The community composition and structure of planktonic heterotrophic bacteria (903 isolates) sampled from a small eutrophic lake in northern England (Priest Pot) was studied with respect to season (four samples) and depth (to 3.1 m). Bacteria (887) were isolated on tryptic soy broth agar and identified to 48 genera usi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2001.tb00815.x

    authors: Edwards ML,Lilley AK,Timms-Wilson TH,Thompson IP,Cooper I

    更新日期:2001-05-01 00:00:00

  • Growing season drives the compositional changes and assembly processes of epiphytic bacterial communities of two submerged macrophytes in Taihu Lake.

    abstract::Large amounts of epiphytic bacteria live on the leaf surfaces of submerged macrophytes in freshwater lakes. Despite their important roles in affecting host plant's health and biogeochemical cycling, knowledge about epiphytic bacteria assembly is not sufficient. We studied epiphytic bacteria on two cohabiting plant spe...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa025

    authors: He D,Ren L,Wu QL

    更新日期:2020-04-01 00:00:00

  • Variations of algal communities cause darkening of a Greenland glacier.

    abstract::We have assessed the microbial ecology on the surface of Mittivakkat glacier in SE-Greenland during the exceptional high melting season in July 2012 when the so far most extreme melting rate for the Greenland Ice Sheet has been recorded. By employing a complementary and multi-disciplinary field sampling and analytical...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12351

    authors: Lutz S,Anesio AM,Jorge Villar SE,Benning LG

    更新日期:2014-08-01 00:00:00

  • Spatial distribution of protists in the presence of macroaggregates in a marine system.

    abstract::The spatial distribution of marine protistan communities in the presence of organic macroaggregates, formed from natural seawater, was studied in several microcosm experiments. The presence of macroaggregates had two main effects. First, the size of the communities of bacteria, flagellates and ciliates increased, as t...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2000.tb00741.x

    authors: Artolozaga I I,Ayo B,Latatu A,Azúa I I,Unanue M,Iriberri J

    更新日期:2000-09-01 00:00:00

  • Succession in the intestinal microbiota of preadolescent turkeys.

    abstract::In the present study, automated ribosomal intergenic spacer analysis (ARISA), library sequence analysis, real-time PCR detection of Bacteroides uniformis and Campylobacter coli and dot-blot hybridizations of Clostridiaceae were used to identify trends in microbial colonization of the ceca of male turkeys. Two separate...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00245.x

    authors: Scupham AJ

    更新日期:2007-04-01 00:00:00

  • Deciphering the genome and secondary metabolome of the plant pathogen Fusarium culmorum.

    abstract::Fusarium culmorum is one of the most important fungal plant pathogens that causes diseases on a wide diversity of cereal and non-cereal crops. We report herein for the first time the genome sequence of F. culmorum strain PV and its associated secondary metabolome that plays a role in the interaction with other microor...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiy078

    authors: Schmidt R,Durling MB,de Jager V,Menezes RC,Nordkvist E,Svatoš A,Dubey M,Lauterbach L,Dickschat JS,Karlsson M,Garbeva P

    更新日期:2018-06-01 00:00:00

  • Microbial dynamics and flagellate grazing during transition to winter in Lakes Hoare and Bonney, Antarctica.

    abstract::The planktonic microbial communities of Lakes Hoare and Bonney were investigated during transition into winter. We hypothesized that the onset of darkness induces changes in the functional role of autotrophic and heterotrophic microplankton. Bacteria decreased in Lake Hoare during March-April, while in Lake Bonney bac...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2012.01423.x

    authors: Thurman J,Parry J,Hill PJ,Priscu JC,Vick TJ,Chiuchiolo A,Laybourn-Parry J

    更新日期:2012-11-01 00:00:00

  • Metaproteomic and metagenomic analyses of defined oceanic microbial populations using microwave cell fixation and flow cytometric sorting.

    abstract::A major obstacle in the molecular investigation of natural, especially oceanic, microbial cells is their adequate preservation for further land-based molecular analyses. Here, we examined the use of microwaves for cell fixation before high-speed flow cytometric sorting to define the metaproteomes and metagenomes of ke...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00927.x

    authors: Mary I,Oliver A,Skipp P,Holland R,Topping J,Tarran G,Scanlan DJ,O'Connor CD,Whiteley AS,Burkill PH,Zubkov MV

    更新日期:2010-10-01 00:00:00

  • The effects of soil horizons and faunal excrement on bacterial distribution in an upland grassland soil.

    abstract::The density and spatial location of bacteria were investigated within different horizons of an upland grassland soil before and after a liming treatment to increase the numbers of large soil fauna. Bacterial cells were located by image analysis of stained thin sections and densities calculated from these data. Excreme...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.10.010

    authors: Bruneau PM,Davidson DA,Grieve IC,Young IM,Nunan N

    更新日期:2005-03-01 00:00:00

  • Exploitation of immunofluorescence for the quantification and characterization of small numbers of Pasteuria endospores.

    abstract::The Pasteuria group of endospore-forming bacteria has been studied as a biocontrol agent of plant-parasitic nematodes. Techniques have been developed for its detection and quantification in soil samples, and these mainly focus on observations of endospore attachment to nematodes. Characterization of Pasteuria populati...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00188.x

    authors: Costa SR,Kerry BR,Bardgett RD,Davies KG

    更新日期:2006-12-01 00:00:00

  • Weekly variations of viruses and heterotrophic nanoflagellates and their potential impact on bacterioplankton in shallow waters of the central Red Sea.

    abstract::Bacterioplankton play a pivotal role in marine ecosystems. However, their temporal dynamics and underlying control mechanisms are poorly understood in tropical regions such as the Red Sea. Here, we assessed the impact of bottom-up (resource availability) and top-down (viruses and heterotrophic nanoflagellates) control...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa033

    authors: Sabbagh EI,Huete-Stauffer TM,Calleja MLL,Silva L,Viegas M,Morán XAG

    更新日期:2020-04-01 00:00:00

  • The bacterial microbiota of Stolotermes ruficeps (Stolotermitidae), a phylogenetically basal termite endemic to New Zealand.

    abstract::Stolotermes ruficeps is a widespread, primitive, lower termite occupying dead and decaying wood of many tree species in New Zealand's temperate forests. We identified core bacterial taxa involved in gut processes through combined DNA- and RNA (cDNA)-based pyrosequencing analysis of the 16S nucleotide sequence from fiv...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12424

    authors: Reid NM,Addison SL,West MA,Lloyd-Jones G

    更新日期:2014-12-01 00:00:00

  • Modification of Escherichia coli-bacteriophage interactions by surfactants and antibiotics in vitro.

    abstract::Although experiments indicate that the abiotic environment plays an important role in bacterial interactions with their parasitic viruses (bacteriophages or phages), it is not yet clear how exposure to compounds present in nature alters the impact of phages on bacterial growth and evolution. To address this question, ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw211

    authors: Scanlan PD,Bischofberger AM,Hall AR

    更新日期:2017-01-01 00:00:00

  • Non-Redfield, nutrient synergy and flexible internal elemental stoichiometry in a marine bacterium.

    abstract::The stoichiometric constraints of algal growth are well understood, whereas there is less knowledge for heterotrophic bacterioplankton. Growth of the marine bacterium Phaeobacter inhibens DSM 17395, belonging to the globally distributed Roseobacter group, was studied across a wide concentration range of NH4+ and PO43-...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix059

    authors: Trautwein K,Feenders C,Hulsch R,Ruppersberg HS,Strijkstra A,Kant M,Vagts J,Wünsch D,Michalke B,Maczka M,Schulz S,Hillebrand H,Blasius B,Rabus R

    更新日期:2017-05-01 00:00:00

  • Comparison of the molecular diversity of the methanogenic community at the brackish and marine ends of a UK estuary.

    abstract::Abstract The 16S rRNA sequence diversity of the euryarchaeal community in a predominately freshwater sediment at East Hill Bridge (EHB) on the River Colne estuary, Essex, UK was investigated and compared to that from marine sediments at the mouth of the river (Colne Point). The East Hill Bridge sediments appear to sup...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2002.tb00902.x

    authors: Purdy KJ,Munson MA,Nedwell DB,Martin Embley T

    更新日期:2002-01-01 00:00:00

  • The effect of soil acidification on atmospheric methane uptake by a Maine forest soil(1).

    abstract::Soil from the zone of maximal methanotrophic activity (approximately 5-8 cm depth) in a mixed coniferous-hardwood forest consumed atmospheric methane over a wide pH range (3.5-7.5) with a broad optimum between 4.8 and 6.0. Methane uptake at native soil pH values (4.4-4.8) was only slightly less rapid than rates at opt...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2001.tb00771.x

    authors: Benstead J,King GM

    更新日期:2001-01-01 00:00:00

  • Characterization and virulence retention of viable but nonculturable Vibrio harveyi.

    abstract::Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. Two clinical V. harveyi strains, SF1 and CW2, and the type strain, VIB295(T), were incubated in sterilized seawater at 4 degrees C. Plate counts in these strains declined to undetectable levels (<0.1 CFU mL(-1)) within 69, 67 and 6...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00442.x

    authors: Sun F,Chen J,Zhong L,Zhang XH,Wang R,Guo Q,Dong Y

    更新日期:2008-04-01 00:00:00

  • Effects of warming and drought on potential N2O emissions and denitrifying bacteria abundance in grasslands with different land-use.

    abstract::Increased warming in spring and prolonged summer drought may alter soil microbial denitrification. We measured potential denitrification activity and denitrifier marker gene abundances (nirK, nirS, nosZ) in grasslands soils in three geographic regions characterized by site-specific land-use indices (LUI) after warming...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv066

    authors: Keil D,Niklaus PA,von Riedmatten LR,Boeddinghaus RS,Dormann CF,Scherer-Lorenzen M,Kandeler E,Marhan S

    更新日期:2015-07-01 00:00:00