Characterization and virulence retention of viable but nonculturable Vibrio harveyi.

Abstract:

:Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. Two clinical V. harveyi strains, SF1 and CW2, and the type strain, VIB295(T), were incubated in sterilized seawater at 4 degrees C. Plate counts in these strains declined to undetectable levels (<0.1 CFU mL(-1)) within 69, 67 and 65 days, respectively. The direct viable count (DVC) declined from 10(6) to 10(4) active cells mL(-1) and remained constant at this level by a DVC. VBNC cell numbers could be restored via a temperature upshift when grown in yeast extract with the addition of Tween 20 or compound vitamin B. Reverse transcriptase-PCR was used to monitor virulence gene expression within VBNC cells. No expression of the hemolysin gene was detected in VBNC cells. VBNC and resuscitative cells were intraperitoneally injected into zebra fish separately. No death was observed in the groups inoculated with VBNC cells. The fish inoculated with the resuscitative cells died within 7 days, the lethal dose 50% (LD(50)) being 2.85 x 10(4) CFU mL(-1), a value similar to that for groups inoculated with normal cells (2.28 x 10(4) CFU mL(-1)). This suggested that VBNC V. harveyi might retain pathogenic potential.

journal_name

FEMS Microbiol Ecol

authors

Sun F,Chen J,Zhong L,Zhang XH,Wang R,Guo Q,Dong Y

doi

10.1111/j.1574-6941.2008.00442.x

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

37-44

issue

1

eissn

0168-6496

issn

1574-6941

pii

FEM442

journal_volume

64

pub_type

杂志文章
  • Transcriptomics analysis of the metabolic mechanisms of iron reduction induced by sulfate reduction mediated by sulfate-reducing bacteria.

    abstract::Sulfate-reducing bacteria (SRB) play an important role in sulfur, iron, and carbon cycling. The majority of studies have illustrated the role of SRB in biogeochemical cycling in pure cultures. In this study, we established three SRB enrichment cultures (designated HL, NB, and WC) from different paddy soils and conduct...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiab005

    authors: Li GX,Bao P

    更新日期:2021-01-13 00:00:00

  • The effect of grazing and nutrient supply on periphyton associated bacteria.

    abstract::The effects of nutrient additions and grazing by macro-invertebrates on periphyton-associated algae and bacteria were studied by performing an enclosure experiment on three occasions from early spring to summer at mesotrophic Lake Erken and Väddö, at the Swedish Baltic coast. There were significant interactions betwee...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.10.003

    authors: Haglund AL,Hillebrand H

    更新日期:2005-03-01 00:00:00

  • Integrating conjugative elements of the SXT/R391 family from fish-isolated Vibrios encode restriction-modification systems that confer resistance to bacteriophages.

    abstract::Integrating conjugative elements (ICEs) of the SXT/R391 family have been described in Vibrios, mainly Vibrio cholerae, and other bacteria as carriers of variable gene content conferring adaptive advantages upon their hosts, including antimicrobial resistance and motility regulation. However, our knowledge on their hos...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12007

    authors: Balado M,Lemos ML,Osorio CR

    更新日期:2013-02-01 00:00:00

  • Impacts of heavy metal contamination and phytoremediation on a microbial community during a twelve-month microcosm experiment.

    abstract::The effects of heavy metals and phytoextraction practices on a soil microbial community were studied during 12 months using a hyperaccumulating plant (Thlaspi caerulescens) grown in an artificially contaminated soil. The 16S ribosomal RNA genes of the Bacteria and the beta-Proteobacteria and the amoA gene (encoding th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.02.004

    authors: Gremion F,Chatzinotas A,Kaufmann K,Von Sigler W,Harms H

    更新日期:2004-05-01 00:00:00

  • Resistance and resilience of Cu-polluted soil after Cu perturbation, tested by a wide range of soil microbial parameters.

    abstract::Copper (Cu)-polluted and unpolluted soils were used to study the effect of initial pollution on soil biological resistance and resilience by measuring the responses to perturbation using different parameters. Microbial biomass carbon, substrate-induced respiration and copy numbers of 16S rRNA gene were grouped as gene...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00741.x

    authors: Deng H,Li XF,Cheng WD,Zhu YG

    更新日期:2009-11-01 00:00:00

  • Physiology of phototrophic iron(II)-oxidizing bacteria: implications for modern and ancient environments.

    abstract::Phototrophic iron(II) [Fe(II)]-oxidizing bacteria are present in modern environments and evidence suggests that this metabolism was present already on early earth. We determined Fe(II) oxidation rates depending on pH, temperature, light intensity, and Fe(II) concentration for three phylogenetically different phototrop...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00592.x

    authors: Hegler F,Posth NR,Jiang J,Kappler A

    更新日期:2008-11-01 00:00:00

  • An appeal for a more evidence based approach to biopesticide safety in the EU.

    abstract::EFSA responded to our perspective article on the safe use of the insect pathogen Bacillus thrurigiensis (Bt). In doing so they admitted that there is no direct evidence to suggest that B. thuringiensis can cause diarrhoea. They nevertheless continue to repeat the assertion that Bt cannot be distinguished from Bacillus...

    journal_title:FEMS microbiology ecology

    pub_type: 评论,信件

    doi:10.1093/femsec/fix169

    authors: Raymond B,Federici BA

    更新日期:2018-01-01 00:00:00

  • Molecular characterization of plasmid-mediated AmpC beta-lactamase- and extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae among corvids (Corvus brachyrhynchos and Corvus corax) roosting in Canada.

    abstract::This study evaluated the carriage of AmpC and extended-spectrum beta-lactamase (ESBL) genes and associated plasmids in faecal bacteria of Canadian corvids. Faecal samples from 449 birds in five roosting sites across Canada were analyzed using selective media, screening for AmpC and ESBL genes by PCR, and sequencing. G...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiy166

    authors: Jamborova I,Janecko N,Halova D,Sedmik J,Mezerova K,Papousek I,Kutilova I,Dolejska M,Cizek A,Literak I

    更新日期:2018-11-01 00:00:00

  • Coral-associated bacterial communities on Ningaloo Reef, Western Australia.

    abstract::Coral-associated microbial communities from three coral species (Pocillopora damicornis, Acropora tenuis and Favites abdita) were examined every 3 months (January, March, June, October) over a period of 1 year on Ningaloo Reef, Western Australia. Tissue from corals was collected throughout the year and additional samp...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00986.x

    authors: Ceh J,Van Keulen M,Bourne DG

    更新日期:2011-01-01 00:00:00

  • More functional genes and convergent overall functional patterns detected by GEOCHIP in phenanthrene-spiked soils.

    abstract::To explore the effect of phenanthrene on the functional diversity of soil microbial communities, Luvisol and Cambisol spiked with phenanthrene and their corresponding control soils were incubated in soil microcosms. Total community DNA extracted from samples taken at days 0 and 21 was analyzed by geochip. The number o...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2012.01413.x

    authors: Ding GC,Heuer H,He Z,Xie J,Zhou J,Smalla K

    更新日期:2012-10-01 00:00:00

  • Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25-3T studied in an in vitro gut model.

    abstract::Butyrate-producing bacteria are promising probiotic candidates to target microbial dysbiosis in gastrointestinal disorders like inflammatory bowel diseases. Butyricicoccus pullicaecorum 25-3(T), a butyrate-producing clostridial cluster IV strain, is such a candidate. Little is known about its abundance in the colon mi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv054

    authors: Geirnaert A,Wang J,Tinck M,Steyaert A,Van den Abbeele P,Eeckhaut V,Vilchez-Vargas R,Falony G,Laukens D,De Vos M,Van Immerseel F,Raes J,Boon N,Van de Wiele T

    更新日期:2015-06-01 00:00:00

  • Mitochondrial sequences of Rhipicephalus and Coxiella endosymbiont reveal evidence of lineages co-cladogenesis.

    abstract::Rhipicephalus ticks are competent vectors of several pathogens, such as Spotted Fever Group Rickettsiae (SFGR) and many Babesia species. Within this genus, different R. sanguineus s.l. lineages show an unequal vector competence and resistance regarding some pathogenic strains. Current literature supports that tick end...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa072

    authors: Coimbra-Dores MJ,Jaarsma RI,Carmo AO,Maia-Silva M,Fonville M,da Costa DFF,Brandão RML,Azevedo F,Casero M,Oliveira AC,Afonso SMS,Sprong H,Rosa F,Dias D

    更新日期:2020-06-01 00:00:00

  • Succession of N cycling processes in biological soil crusts on a Central European inland dune.

    abstract::Biological soil crusts (BSCs) are microbial assemblages that occur worldwide and facilitate ecosystem development by nitrogen (N) and carbon accumulation. N turnover within BSC ecosystems has been intensively studied in the past; however, shifts in the N cycle during BSC development have not been previously investigat...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2012.01459.x

    authors: Brankatschk R,Fischer T,Veste M,Zeyer J

    更新日期:2013-01-01 00:00:00

  • Growth, lipid accumulation, and fatty acid composition in obligate psychrophilic, facultative psychrophilic, and mesophilic yeasts.

    abstract::Obligate psychrophilic, facultative psychrophilic, and mesophilic yeasts were cultured in a carbon-rich medium at different temperatures to investigate whether growth parameters, lipid accumulation, and fatty acid (FA) composition were adaptive and/or acclimatory responses. Acclimation of facultative psychrophiles and...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00727.x

    authors: Rossi M,Buzzini P,Cordisco L,Amaretti A,Sala M,Raimondi S,Ponzoni C,Pagnoni UM,Matteuzzi D

    更新日期:2009-09-01 00:00:00

  • Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatum truffle-ground.

    abstract::Truffles are hypogeous ectomycorrhizal fungi. They belong to the genus Tuber and are currently considered a hot spot in fungal biology due to their ecological and economic relevance. Among all the species, Tuber magnatum is the most appreciated because of its special taste and aroma. The aim of this work was to set up...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00783.x

    authors: Zampieri E,Murat C,Cagnasso M,Bonfante P,Mello A

    更新日期:2010-01-01 00:00:00

  • Investigations of Rhizobium biofilm formation.

    abstract::The development of nitrogen-fixing nodules of the Rhizobium-legume symbiosis, especially the early stages of root hair deformation and curling, infection thread formation, and nodule initiation, has been well studied from a genetic standpoint. In contrast, the factors important for the colonization of surfaces by rhiz...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2005.00044.x

    authors: Fujishige NA,Kapadia NN,De Hoff PL,Hirsch AM

    更新日期:2006-05-01 00:00:00

  • Three types of phenol and p-cresol catabolism in phenol- and p-cresol-degrading bacteria isolated from river water continuously polluted with phenolic compounds.

    abstract::A total of 39 phenol- and p-cresol-degraders isolated from the river water continuously polluted with phenolic compounds of oil shale leachate were studied. Species identification by BIOLOG GN analysis revealed 21 strains of Pseudomonas fluorescens (4, 8 and 9 of biotypes A, C and G, respectively), 12 of Pseudomonas m...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2000.tb00684.x

    authors: Heinaru E,Truu J,Stottmeister U,Heinaru A

    更新日期:2000-03-01 00:00:00

  • Members of Microvirga and Bradyrhizobium genera are native endosymbiotic bacteria nodulating Lupinus luteus in Northern Tunisian soils.

    abstract::The genetic diversity of bacterial populations nodulating Lupinus luteus (yellow lupine) in Northern Tunisia was examined. Phylogenetic analyses of 43 isolates based on recA and gyrB partial sequences grouped them in three clusters, two of which belong to genus Bradyrhizobium (41 isolates) and one, remarkably, to Micr...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix068

    authors: Msaddak A,Rejili M,Durán D,Rey L,Imperial J,Palacios JM,Ruiz-Argüeso T,Mars M

    更新日期:2017-06-01 00:00:00

  • Effects of host traits and land-use changes on the gut microbiota of the Namibian black-backed jackal (Canis mesomelas).

    abstract::Host traits and environmental factors drive the natural variation in gut microbiota, and disruption in homeostasis can cause infections and chronic diseases. African wildlife is increasingly facing human-induced agricultural habitats, which also amplifies the contact probability with livestock with unknown consequence...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix123

    authors: Menke S,Meier M,Mfune JKE,Melzheimer J,Wachter B,Sommer S

    更新日期:2017-11-01 00:00:00

  • DNA stable-isotope probing identifies uncultivated members of Pseudonocardia associated with biodegradation of pyrene in agricultural soil.

    abstract::Identifying functional microorganisms involved in the degradation of high-molecular-mass polycyclic aromatic hydrocarbons (HMM-PAHs) in agricultural soil environments could assist in developing bioremediation strategies for soil PAH contamination. Active populations of HMM-PAH degraders in agricultural soils are curre...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiy026

    authors: Chen SC,Duan GL,Ding K,Huang FY,Zhu YG

    更新日期:2018-03-01 00:00:00

  • Weekly variations of viruses and heterotrophic nanoflagellates and their potential impact on bacterioplankton in shallow waters of the central Red Sea.

    abstract::Bacterioplankton play a pivotal role in marine ecosystems. However, their temporal dynamics and underlying control mechanisms are poorly understood in tropical regions such as the Red Sea. Here, we assessed the impact of bottom-up (resource availability) and top-down (viruses and heterotrophic nanoflagellates) control...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa033

    authors: Sabbagh EI,Huete-Stauffer TM,Calleja MLL,Silva L,Viegas M,Morán XAG

    更新日期:2020-04-01 00:00:00

  • Effects of transgenic fructan-producing potatoes on the community structure of rhizosphere and phyllosphere bacteria.

    abstract::The rhizosphere and phyllosphere microbial communities of transgenic potatoes producing fructan were studied in comparison with isogenic controls and conventional varieties in a field release experiment over a period of 3 years. Population densities and 16S rRNA gene-based terminal restriction fragment length polymorp...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00562.x

    authors: Becker R,Behrendt U,Hommel B,Kropf S,Ulrich A

    更新日期:2008-11-01 00:00:00

  • A bacterial culture-independent method to investigate chemically mediated control of bacterial epibiosis in marine invertebrates by using TRFLP analysis and natural bacterial populations.

    abstract::It has been postulated that a variety of physically undefended marine invertebrates have evolved strategies to control microbial epibiosis chemically. Ecologically meaningful experiments that demonstrate chemically mediated antibacterial effects are difficult due to the small number of cultivable bacteria. Based on th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00246-0

    authors: Harder T,Lau SC,Tam WY,Qian PY

    更新日期:2004-01-01 00:00:00

  • Rhizosphere persistence of three Pythium oligandrum strains in tomato soilless culture assessed by DNA macroarray and real-time PCR.

    abstract::In tomato soilless culture, plant-disease optimal control and growth promotion are achieved when the rhizosphere is heavily colonized by the biocontrol agent Pythium oligandrum. Discrepancies in performance are generally attributed to the poor persistence of P. oligandrum on roots. In this study, three selected strain...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2007.00348.x

    authors: Le Floch G,Tambong J,Vallance J,Tirilly Y,Lévesque A,Rey P

    更新日期:2007-08-01 00:00:00

  • Phylogenetic and clonality analysis of Bacillus pumilus isolates uncovered a highly heterogeneous population of different closely related species and clones.

    abstract::Bacillus pumilus is a Gram-positive bacterium with a wide range of attributed applications, namely as a plant growth promoting rhizobacteria (PGPR), animal, and human probiotic. However, a rare putative role in human diseases has been reported, namely in food poisoning or as anthrax-like cutaneous infectious agent. Th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12426

    authors: Branquinho R,Meirinhos-Soares L,Carriço JA,Pintado M,Peixe LV

    更新日期:2014-12-01 00:00:00

  • Absence of carbon transfer between Medicago truncatula plants linked by a mycorrhizal network, demonstrated in an experimental microcosm.

    abstract::Carbon transfer between plants via a common extraradical network of arbuscular mycorrhizal (AM) fungal hyphae has been investigated abundantly, but the results remain equivocal. We studied the transfer of carbon through this fungal network, from a Medicago truncatula donor plant to a receiver (1) M. truncatula plant g...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00503.x

    authors: Voets L,Goubau I,Olsson PA,Merckx R,Declerck S

    更新日期:2008-08-01 00:00:00

  • Mathematical modelling of antimicrobial resistance in agricultural waste highlights importance of gene transfer rate.

    abstract::Antimicrobial resistance is of global concern. Most antimicrobial use is in agriculture; manures and slurry are especially important because they contain a mix of bacteria, including potential pathogens, antimicrobial resistance genes and antimicrobials. In many countries, manures and slurry are stored, especially ove...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw040

    authors: Baker M,Hobman JL,Dodd CE,Ramsden SJ,Stekel DJ

    更新日期:2016-04-01 00:00:00

  • Investigations into peach seedling stunting caused by a replant soil.

    abstract::Replant diseases often occur when pome and stone fruits are grown in soil that had previously been planted with the same or similar plant species. They typically lead to reductions in plant growth, crop yield and production duration. In this project, greenhouse assays were used to identify a peach orchard soil that ca...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00668.x

    authors: Bent E,Loffredo A,Yang JI,McKenry MV,Becker JO,Borneman J

    更新日期:2009-05-01 00:00:00

  • Elsinochrome A production by the bindweed biocontrol fungus Stagonospora convolvuli LA39 does not pose a risk to the environment or the consumer of treated crops.

    abstract::Biological control as an alternative to chemical pesticides is of increasing public interest. However, to ensure safe use of biocontrol methods, strategies to assess the possible risks need to be developed. The production of toxic metabolites is an aspect which has so far largely been neglected in the risk assessment ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00207.x

    authors: Boss D,Maurhofer M,Schläpfer E,Défago G

    更新日期:2007-01-01 00:00:00

  • The enrichment of an alkaliphilic biofilm consortia capable of the anaerobic degradation of isosaccharinic acid from cellulosic materials incubated within an anthropogenic, hyperalkaline environment.

    abstract::Anthropogenic hyperalkaline sites provide an environment that is analogous to proposed cementitious geological disposal facilities (GDF) for radioactive waste. Under anoxic, alkaline conditions cellulosic wastes will hydrolyze to a range of cellulose degradation products (CDP) dominated by isosaccharinic acids (ISA). ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv085

    authors: Charles CJ,Rout SP,Garratt EJ,Patel K,Laws AP,Humphreys PN

    更新日期:2015-08-01 00:00:00