Exploitation of immunofluorescence for the quantification and characterization of small numbers of Pasteuria endospores.

Abstract:

:The Pasteuria group of endospore-forming bacteria has been studied as a biocontrol agent of plant-parasitic nematodes. Techniques have been developed for its detection and quantification in soil samples, and these mainly focus on observations of endospore attachment to nematodes. Characterization of Pasteuria populations has recently been performed with DNA-based techniques, which usually require the extraction of large numbers of spores. We describe a simple immunological method for the quantification and characterization of Pasteuria populations. Bayesian statistics were used to determine an extraction efficiency of 43% and a threshold of detection of 210 endospores g(-1) sand. This provided a robust means of estimating numbers of endospores in small-volume samples from a natural system. Based on visual assessment of endospore fluorescence, a quantitative method was developed to characterize endospore populations, which were shown to vary according to their host.

journal_name

FEMS Microbiol Ecol

authors

Costa SR,Kerry BR,Bardgett RD,Davies KG

doi

10.1111/j.1574-6941.2006.00188.x

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

593-600

issue

3

eissn

0168-6496

issn

1574-6941

pii

FEM188

journal_volume

58

pub_type

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