Characterization of methanogenic Archaea in the leachate of a closed municipal solid waste landfill.

Abstract:

:Cultivation-independent molecular approaches were used to investigate the phylogenetic composition of Archaea and the relative abundance of phylogenetically defined groups of methanogens in the leachate of a closed municipal solid waste landfill. Cloning and phylogenetic analysis of archaeal 16S rRNA gene sequences (16S rDNA) revealed that the landfill leachate harbored a diverse Archaea community, with sequence types distributed within the two archaeal kingdoms of the Euryarchaeota and the Crenarchaeota. Of the 80 clones examined, 51 were phylogenetically associated with well-defined methanogen lineages covering two major methanogenic phenotypes; 20 were related to Thermoplasma and were grouped with some novel archaeal rRNA gene sequences recently recovered from various anaerobic habitats; finally, five belonged to Crenarchaeota and were not closely related to any hitherto cultivated species. Most of the methanogen-like clones were affiliated with the hydrogenotrophic Methanomicrobiales and the methylotrophic and acetoclastic Methanosarcinales. Quantitative oligonucleotide hybridization experiments showed that methanogens in the leachate accounted for only a very small fraction of the total community (approximately 2%) and that Methanomicrobiales and Methanosarcinales constituted the majority of the total methanogenic population.

journal_name

FEMS Microbiol Ecol

authors

Huang LN,Chen YQ,Zhou H,Luo S,Lan CY,Qu LH

doi

10.1016/S0168-6496(03)00218-6

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

171-7

issue

2

eissn

0168-6496

issn

1574-6941

pii

FEM171

journal_volume

46

pub_type

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