Abstract:
:A modified fluorescence in situ hybridization (FISH) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. Under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-Proteobacteria -- Aeromonas hydrophila and Pseudomonas fluorescens, respectively) to grazing by three protists: the flagellates Bodo saltans and Goniomonas sp., and the ciliate Cyclidium glaucoma. Both flagellate species preferably ingested A. hydrophila over P. fluorescens, while C. glaucoma showed no clear preferences. Differences were found in the digestion of bacterial prey with B. saltans digesting significantly faster P. fluorescens compared to two other protists. The field study was conducted in a reservoir as part of a larger experiment. We monitored changes in the bacterial prey composition available compared to the bacteria ingested in flagellate food vacuoles. Bacteria detected by probe HGC69a (Actinobacteria) and R-BT065 were negatively selected by flagellates. Bacteria detected by probe CF319a were initially positively selected but along with a temporal shift in bacterial cell size, this trend changed to negative selection during the experiment. Overall, our analysis of protistan food vacuole content indicated marked effects of flagellate prey selectivity on bacterioplankton community composition.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Jezbera J,Hornák K,Simek Kdoi
10.1016/j.femsec.2004.12.001subject
Has Abstractpub_date
2005-05-01 00:00:00pages
351-63issue
3eissn
0168-6496issn
1574-6941pii
S0168-6496(04)00358-7journal_volume
52pub_type
杂志文章abstract::The aim of this work was to relate depth profiles of prokaryotic community composition with geochemical processes in the deep subseafloor biosphere at two shallow-water sites on the Peru Margin in the Pacific Ocean (ODP Leg 201, sites 1228 and 1229). Principal component analysis of denaturing gradient gel electrophore...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2006.00144.x
更新日期:2006-10-01 00:00:00
abstract::Arbuscular mycorrhizal (AM) fungi in a chronosequence of 5-42-year-old Caragana korshinskii plantations in the semi-arid Loess Plateau region of northwestern China were investigated. AM fungi colonization, spore diversity and PCR-denatured gradient gel electrophoresis-based AM fungal SSU rRNA gene sequences were analy...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2008.00597.x
更新日期:2009-01-01 00:00:00
abstract::The Pasteuria group of endospore-forming bacteria has been studied as a biocontrol agent of plant-parasitic nematodes. Techniques have been developed for its detection and quantification in soil samples, and these mainly focus on observations of endospore attachment to nematodes. Characterization of Pasteuria populati...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2006.00188.x
更新日期:2006-12-01 00:00:00
abstract::The bias of widely used degenerate nifH-specific primer sets was first tested using denaturing gradient gel electrophoresis (DGGE), and their application for profiling of complex communities assessed for roots of Oryza longistaminata. When primers (P) with mismatches at nondegenerate positions were used on genomic DNA...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2008.00545.x
更新日期:2008-08-01 00:00:00
abstract::Denitrifying prokaryotes are phylogenetically and functionally diverse. Little is known about the relationship between soil denitrifier community composition and functional traits. We extracted bacterial cells from three cultivated peat soils with contrasting native pH by density gradient centrifugation and investigat...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01233.x
更新日期:2012-02-01 00:00:00
abstract::In an effort to identify the causes and patterns of temporal change in periphytic communities, we examined biomass accumulation, taxonomic and functional composition, rate of species turnover, and pairwise species correlations in response to variability in current velocity and nutrient supply in artificial stream flum...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01302.x
更新日期:2012-05-01 00:00:00
abstract::The stoichiometric constraints of algal growth are well understood, whereas there is less knowledge for heterotrophic bacterioplankton. Growth of the marine bacterium Phaeobacter inhibens DSM 17395, belonging to the globally distributed Roseobacter group, was studied across a wide concentration range of NH4+ and PO43-...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fix059
更新日期:2017-05-01 00:00:00
abstract::Biological control as an alternative to chemical pesticides is of increasing public interest. However, to ensure safe use of biocontrol methods, strategies to assess the possible risks need to be developed. The production of toxic metabolites is an aspect which has so far largely been neglected in the risk assessment ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2006.00207.x
更新日期:2007-01-01 00:00:00
abstract::Cyanobacterial blooms have become more frequent as a result of eutrophication in lakes. The accumulation and breakdown of huge cyanobacterial biomasses often cause hypoxia in lakes. However, little is known about microbial diversity in these areas. In this study, we characterized the bacterial community composition of...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01262.x
更新日期:2012-03-01 00:00:00
abstract::Marine macroalgae are subjected to large numbers of bacteria in their environment. These bacteria have the potential to affect the health and ecology of algae in a variety of ways and can be both beneficial and harmful to the algae. Therefore, algae have likely evolved mechanisms to differentially regulate the growth ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2010.01005.x
更新日期:2011-02-01 00:00:00
abstract::Photosynthetic picoeukaryotes (PPEs) are major oceanic primary producers. However, the diversity of such communities remains poorly understood, especially in the northwestern (NW) Pacific. We investigated the abundance and diversity of PPEs, and recorded environmental variables, along a transect from the coast to the ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiv170
更新日期:2016-02-01 00:00:00
abstract::A particular fungal population is present in the main stages of the manufacturing process of cork discs. Its diversity was studied using both dependent (isolation) and independent culture methods (denaturing gel gradient electrophoresis and cloning of the ITS1-5.8S-ITS2 region). The mycobiota in the samples taken in t...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01419.x
更新日期:2012-10-01 00:00:00
abstract::Ectomycorrhizal fungi (EMF) provide nutrients to their hosts by means of hyphae that extend beyond nutrient-depleted rhizosphere soil. Soil bacteria may compete with EMF for nutrients or may act synergistically to enhance nutrient supply to hosts. To assess the interactions between hyphae and bacteria, two types of sm...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01060.x
更新日期:2011-05-01 00:00:00
abstract::Biological soil crusts (BSCs) are microbial assemblages that occur worldwide and facilitate ecosystem development by nitrogen (N) and carbon accumulation. N turnover within BSC ecosystems has been intensively studied in the past; however, shifts in the N cycle during BSC development have not been previously investigat...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01459.x
更新日期:2013-01-01 00:00:00
abstract::Differences between endophytic and ectophytic bacterial communities with stress on antagonistic bacteria, were studied by comparing the composition of communities isolated from the rhizosphere, phyllosphere, endorhiza and endosphere of field-grown potato plants using a multiphasic approach. Terminal restriction fragme...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1016/j.femsec.2004.08.006
更新日期:2005-01-01 00:00:00
abstract::Integrating conjugative elements (ICEs) of the SXT/R391 family have been described in Vibrios, mainly Vibrio cholerae, and other bacteria as carriers of variable gene content conferring adaptive advantages upon their hosts, including antimicrobial resistance and motility regulation. However, our knowledge on their hos...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/1574-6941.12007
更新日期:2013-02-01 00:00:00
abstract::The influence of biotic and environmental variables on the abundance of virus-like particles (VLP) and lysogeny was investigated by examining 10 Antarctic lakes in the Vestfold Hills, Antarctica, in the Austral Spring. Abundances of viruses and bacteria and bacterial metabolic activity were estimated using SYBR Gold (...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2007.00407.x
更新日期:2008-01-01 00:00:00
abstract::We report first insights into a representative genome of rice cluster I (RC-I), a major group of as-yet uncultured methanogens. The starting point of our study was the methanogenic consortium MRE50 that had been stably maintained for 3 years by consecutive transfers to fresh medium and anaerobic incubation at 50 degre...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1016/j.femsec.2004.12.004
更新日期:2005-07-01 00:00:00
abstract::We present an individual-based experimental framework to identify and estimate the main parameters governing bacterial conjugation at the individual cell scale. From this analysis, we have established that transient periods of unregulated plasmid transfer within newly formed transconjugant cells, together with contact...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2010.00994.x
更新日期:2011-01-01 00:00:00
abstract::Aerobic anoxygenic phototrophic bacteria may play a particular role in carbon cycling of aquatic systems. However, little is known about the interaction between aerobic anoxygenic phototrophic bacteria and hydrochemistry in groundwater-surface water exchange systems of subtropical karst catchments. We carried out a de...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fix065
更新日期:2017-06-01 00:00:00
abstract::The diversity of protists was researched in the Alboran Sea (SW Mediterranean Sea) by means of high-throughput sequencing technologies based on the amplification of the V9 region of 18S rRNA. Samples were collected at different depths in seven stations following an environmental gradient from a coastal upwelling zone ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiaa197
更新日期:2020-10-20 00:00:00
abstract::Biogeographic patterns in microbial communities are an exciting but controversial topic in microbial ecology. Advances in theory pertaining to assembly of microbial communities have made strong assumptions about dispersal of bacteria without exploration. For this reason, we investigated rates of atmospheric bacterial ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2008.00476.x
更新日期:2008-06-01 00:00:00
abstract::Terminal-restriction fragment length polymorphism (T-RFLP) analysis is widely used in microbial ecology studies. In the present study, T-RFLP analysis of PCR products digested by five restriction enzymes (AluI, HaeIII, MspI, Sau3AI and TaqI) was applied for 20 samples from three contrasting coastal environments to ass...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2008.00493.x
更新日期:2008-07-01 00:00:00
abstract::Bacteria catalyze the dissolution and re-precipitation of gold, thereby driving the biogeochemical cycle of gold. Dissolution of gold/silver and re-precipitation of gold transforms gold particles by increasing gold purity. While soluble gold complexes are highly cytotoxic, little is known about how gold cycling affect...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiz078
更新日期:2019-07-01 00:00:00
abstract::Maintaining beneficial interactions with microbial symbionts is vital for animal health. Yet, for social insects, the stability of microbial associations within and between cohorts is largely unknown. We investigated temporal changes in the microbiomes of nine stingless bee (Tetragonula carbonaria) colonies at seven t...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiaa236
更新日期:2020-12-30 00:00:00
abstract::Flagellates are very important predators on bacteria in soil. Because of their high growth rates, flagellate populations respond rapidly to changes in bacterial numbers. Previous results indicate that actinobacteria are generally less suitable than proteobacteria as food for flagellates. In this study, we investigated...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2008.00486.x
更新日期:2008-07-01 00:00:00
abstract::This study evaluated the carriage of AmpC and extended-spectrum beta-lactamase (ESBL) genes and associated plasmids in faecal bacteria of Canadian corvids. Faecal samples from 449 birds in five roosting sites across Canada were analyzed using selective media, screening for AmpC and ESBL genes by PCR, and sequencing. G...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiy166
更新日期:2018-11-01 00:00:00
abstract::Knowledge of microbial communities and their inherent heterogeneity has dramatically increased with the widespread use of high-throughput sequencing technologies, and we are learning more about the ecological processes that structure microbial communities across a wide range of environments, as well as the relative sc...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01416.x
更新日期:2012-10-01 00:00:00
abstract::The annually reoccurring blooms that characterize the surface waters of the Baltic Sea are dominated by filamentous, heterocystous cyanobacteria such as Nodularia spumigena. In a previous study, we have demonstrated that N. spumigena strain AV1 differentiates heterocysts in the absence of detectable nitrogen fixation ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01127.x
更新日期:2011-08-01 00:00:00
abstract::Prolonged lack of enteral feeding has a negative impact on gut physiology, potentially via microbiota modulation. The aims were to investigate the impact of fasting and post-prandial changes in canine jejunal microbiota. To study post-prandial effects, jejunal brushings were analyzed in 8 healthy fistulated dogs 15 mi...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiw085
更新日期:2016-06-01 00:00:00