Abstract:
:Population levels of total and ampicillin-resistant culturable bacteria and the putative horizontal bla gene acquirement from Bt-corn were studied in commercial fields of transgenic corn in Spain during the years 2000-2003. Commercial fields consisting of conventional corn (Dracma) and Bt176 transgenic corn (Compa CB) were located in three climatic regions. The effect of corn type, plant material, field location, stage of sampling and year of study were studied on total and ampicillin resistant bacterial population levels, on median effective dose and on the slope of the dose-response curve to ampicillin. None of the parameters measured were significantly different (P<0.01) between transgenic and non-transgenic cornfields under the diverse conditions studied. However, in population levels of ampicillin resistant bacteria, the minimum difference between sample means to be significant with a likelihood of 80% was 8.9%. Specific detection of putative bacteria harbouring bla TEM-1 ampicillin resistance genes acquired from Bt176 corn was performed with a method based on the extraction of DNA from the culturable bacterial fraction and with PCR. Primers for PCR were targeted to the bla gene and the corresponding flanking regions present in the pUC18 cloning vector or the Bt176 construct. The culturable bacterial fraction of 144 field samples (up to 864 analysis, including ampicillin enrichments) was analysed by PCR. The estimated total number of bacteria analysed was 10(8). The level of detection of a transfer event according to the sensitivity of the methods used was 10(-6). Four samples of transgenic and five of non-transgenic corn gave positive signals. However, the amplification products did not correspond to the ones expected from Bt176 or pUC18. The limitations of the sampling design and of the methods used are discussed.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Badosa E,Moreno C,Montesinos Edoi
10.1016/j.femsec.2004.01.005subject
Has Abstractpub_date
2004-05-01 00:00:00pages
169-78issue
2eissn
0168-6496issn
1574-6941pii
FEM169journal_volume
48pub_type
杂志文章abstract::Denitrifying prokaryotes are phylogenetically and functionally diverse. Little is known about the relationship between soil denitrifier community composition and functional traits. We extracted bacterial cells from three cultivated peat soils with contrasting native pH by density gradient centrifugation and investigat...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01233.x
更新日期:2012-02-01 00:00:00
abstract::The polychlorinated biphenyl (PCB)-degrading Pseudomonas sp. B4 was tested for its motility and ability to sense and respond to biphenyl, its chloroderivatives and chlorobenzoates in chemotaxis assays. Pseudomonas sp. B4 was attracted to biphenyl, PCBs and benzoate in swarm plate and capillary assays. Chemotaxis towar...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2007.00293.x
更新日期:2007-05-01 00:00:00
abstract::Plasmid-mediated horizontal gene transfer influences bacterial community structure and evolution. However, an understanding of the forces which dictate the fate of plasmids in bacterial populations remains elusive. This is in part due to the enormous diversity of plasmids, in terms of size, structure, transmission, ev...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6941.2008.00505.x
更新日期:2008-10-01 00:00:00
abstract::Coral reefs are among the most productive ecosystems on the planet, but are rapidly declining due to global-warming-mediated changes in the oceans. Particularly for the Caribbean region, Acropora sp. stony corals have lost ∼80% of their original coverage, resulting in vast extensions of dead coral rubble. We analyzed ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiv162
更新日期:2016-01-01 00:00:00
abstract::DNA sequencing technology has proven very valuable for analysing the microbiota of poorly accessible ecosystems such as hydrothermal vents. Using a combination of amplicon and shotgun sequencing of small-subunit rRNA and its gene, we examined the composition and diversity of microbial communities from the recently dis...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01138.x
更新日期:2011-09-01 00:00:00
abstract::Organic soil fertilizers, such as livestock manure and biogas digestate, frequently contain bacteria carrying resistance genes (RGs) to antimicrobial substances and mobile genetic elements (MGEs). The effects of different fertilizers (inorganic, manure, digestate) on RG and MGE abundance and microbial community compos...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiy027
更新日期:2018-04-01 00:00:00
abstract::It has been postulated that a variety of physically undefended marine invertebrates have evolved strategies to control microbial epibiosis chemically. Ecologically meaningful experiments that demonstrate chemically mediated antibacterial effects are difficult due to the small number of cultivable bacteria. Based on th...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1016/S0168-6496(03)00246-0
更新日期:2004-01-01 00:00:00
abstract::Biodiversity patterns have been successfully linked to many ecosystem functions, and microbial communities have been suspected to harbour a large amount of functionally redundant taxa. We manipulated the diversity of stream and lake water column bacterial communities and investigated how the reduction in diversity aff...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/1574-6941.12101
更新日期:2013-07-01 00:00:00
abstract::Potato cultivation has a strategic role as a food source for the human population. Its promising future development relies on improving the control of the numerous microbial diseases that affect its growth. Numerous and recent studies on the potato rhizosphere, mycorrhizosphere and endorhiza reveal the presence of a d...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6941.2010.01023.x
更新日期:2011-03-01 00:00:00
abstract::Land plants directly contact soil through their roots. An enormous diversity of microbes dwelling in root-associated zones, including endosphere (inside root), rhizoplane (root surface) and rhizosphere (soil surrounding the root surface), play essential roles in ecosystem functioning and plant health. Rice is a staple...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiz040
更新日期:2019-05-01 00:00:00
abstract::Bioavailability of electron acceptors is probably the most limiting factor in the restoration of anoxic, contaminated environments. The oxidation of contaminants such as aromatic hydrocarbons, particularly in aquifers, often depends on the reduction of ferric iron or sulphate. We have previously detected a highly acti...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01083.x
更新日期:2011-10-01 00:00:00
abstract::Turf, consisting of closely spaced grasses and the subtending soil, is a unique ecosystem subject to intense management. Yet soil organic matter accumulates quickly and reaches equilibrium after 20 to 50 years. Resource availability is an important driver of species richness and theoretically their relationship is exp...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiy224
更新日期:2019-02-01 00:00:00
abstract::Methanogenic biodegradation involves the cooperative metabolism of syntrophic bacteria that catalyse the initial attack and subsequent degradation of hydrocarbons, and methanogens that convert intermediates such as hydrogen and carbon dioxide, formate, and/or acetate to methane. The identity of syntrophic microbes and...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/1574-6941.12364
更新日期:2014-09-01 00:00:00
abstract::Complex interactions between plants and soil microorganisms drive key ecosystem and community properties such as productivity and diversity. In nutrient-poor systems such as sand dunes, plant traits and fungal symbioses related to nutrient acquisition can strongly influence vegetation dynamics. We investigated plant a...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiw156
更新日期:2016-10-01 00:00:00
abstract::In contrast to the pervasive occurrence of denitrification in soils, anammox (anaerobic ammonium oxidation) is a spatially restricted process that depends on specific ecological conditions. To identify the factors that constrain the distribution and activity of anammox bacteria in terrestrial environments, we investig...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiz191
更新日期:2020-02-01 00:00:00
abstract::Phytate-mineralizing rhizobacteria (PMR) perform an essential function for the mineralization of organic phosphorus but little is known about their ecology in soils and rhizosphere. In this study, PCR-based methods were developed for detection and quantification of the Bacillus β-propeller phytase (BPP) gene. Experime...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01480.x
更新日期:2013-02-01 00:00:00
abstract::This study analysed the nitrifier community in the biofilter of a zero discharge, recirculating aquaculture system (RAS) for the production of marine shrimp in a low density (low ammonium production) system. The ammonia-oxidizing populations were examined by targeting 16S rRNA and amoA genes of ammonia-oxidizing bacte...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01448.x
更新日期:2013-01-01 00:00:00
abstract::Soil RNA and DNA were coextracted along a contamination gradient at a landfarming field with aged crude oil contamination to investigate pollution-dependent differences in 16S rRNA and rRNA gene pools. Microbial biomass correlated with nucleic acid yields as well as bacterial community change, indicating that the same...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/1574-6941.12376
更新日期:2014-10-01 00:00:00
abstract::This study examined the effects of temporal changes in bacterial community composition (BCC) and environmental factors on potential ectoenzymatic activities (alpha-glucosidase, beta-glucosidase, alkaline phosphatase and leucine aminopeptidase) in a lacustrine ecosystem (Sep reservoir, France). BCC was assessed by term...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2009.00730.x
更新日期:2009-10-01 00:00:00
abstract::High-elevation lakes in the tropics are subject to extreme environmental fluctuations and microbes may harbor a unique genomic repertoire, but their composition and diversity are largely unknown. Here, we compared the planktonic bacterial community composition (BCC) and diversity of three tropical lakes located in the...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiy004
更新日期:2018-03-01 00:00:00
abstract::Denaturing gradient gel electrophoresis (DGGE) was employed to resolve PCR-amplified nifH sequences from vegetated and unvegetated sediments from two oligotrophic seagrass bed sites on San Salvador Island, Bahamas, in order to assess diazotroph species composition. All DGGE profiles from these sites showed the same pr...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2002.tb00912.x
更新日期:2002-02-01 00:00:00
abstract::Rhipicephalus ticks are competent vectors of several pathogens, such as Spotted Fever Group Rickettsiae (SFGR) and many Babesia species. Within this genus, different R. sanguineus s.l. lineages show an unequal vector competence and resistance regarding some pathogenic strains. Current literature supports that tick end...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiaa072
更新日期:2020-06-01 00:00:00
abstract::The dissemination of extended-spectrum β-lactamases (ESBLs) genes among bacteria is commonly achieved by plasmid conjugation. In the last decade, the CTX-M type enzyme was the most widespread and prevalent ESBLs in the world. In Hong Kong and mainland China, among the commonly found CTX-M-carrying plasmids were pHK01 ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fix090
更新日期:2017-07-01 00:00:00
abstract::Drift ice, open water and under-ice water bacterial communities covering several developmental stages from open water to thick ice were studied in the northern Baltic Sea. The bacterial communities were assessed with 16S rRNA gene terminal-restriction fragment length polymorphism and cloning, together with bacterial a...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiu022
更新日期:2015-02-01 00:00:00
abstract::There is interest in understanding how cultivation, plant genotype, climate and soil conditions influence the biogeography of root nodule bacterial communities of legumes. For crops from regions with relict wild populations, this is of even greater interest because the effects of cultivation on symbiont communities ca...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiaa145
更新日期:2020-10-01 00:00:00
abstract::EFSA responded to our perspective article on the safe use of the insect pathogen Bacillus thrurigiensis (Bt). In doing so they admitted that there is no direct evidence to suggest that B. thuringiensis can cause diarrhoea. They nevertheless continue to repeat the assertion that Bt cannot be distinguished from Bacillus...
journal_title:FEMS microbiology ecology
pub_type: 评论,信件
doi:10.1093/femsec/fix169
更新日期:2018-01-01 00:00:00
abstract::In eastern Canada, climate change-related warming and increased precipitation may alter winter snow cover, with potential consequences for soil conditions, nitrogen (N) cycling, and microbes. We conducted a 2-year field study aimed at determining the influence of snow removal, snow accumulation, and ambient snow in a ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiz073
更新日期:2019-07-01 00:00:00
abstract::A large amount of sequencing data is produced in microbial community ecology studies using the high-throughput sequencing technique, especially amplicon-sequencing-based community data. After conducting the initial bioinformatic analysis of amplicon sequencing data, performing the subsequent statistics and data mining...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiaa255
更新日期:2020-12-17 00:00:00
abstract::We have assessed the microbial ecology on the surface of Mittivakkat glacier in SE-Greenland during the exceptional high melting season in July 2012 when the so far most extreme melting rate for the Greenland Ice Sheet has been recorded. By employing a complementary and multi-disciplinary field sampling and analytical...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/1574-6941.12351
更新日期:2014-08-01 00:00:00
abstract::Kombucha, historically an Asian tea-based fermented drink, has recently become trendy in Western countries. Producers claim it bears health-enhancing properties that may come from the tea or metabolites produced by its microbiome. Despite its long history of production, microbial richness and dynamics have not been fu...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fix048
更新日期:2017-05-01 00:00:00