Unraveling microbial ecology of industrial-scale Kombucha fermentations by metabarcoding and culture-based methods.

Abstract:

:Kombucha, historically an Asian tea-based fermented drink, has recently become trendy in Western countries. Producers claim it bears health-enhancing properties that may come from the tea or metabolites produced by its microbiome. Despite its long history of production, microbial richness and dynamics have not been fully unraveled, especially at an industrial scale. Moreover, the impact of tea type (green or black) on microbial ecology was not studied. Here, we compared microbial communities from industrial-scale black and green tea fermentations, still traditionally carried out by a microbial biofilm, using culture-dependent and metabarcoding approaches. Dominant bacterial species belonged to Acetobacteraceae and to a lesser extent Lactobacteriaceae, while the main identified yeasts corresponded to Dekkera, Hanseniaspora and Zygosaccharomyces during all fermentations. Species richness decreased over the 8-day fermentation. Among acetic acid bacteria, Gluconacetobacter europaeus, Gluconobacter oxydans, G. saccharivorans and Acetobacter peroxydans emerged as dominant species. The main lactic acid bacteria, Oenococcus oeni, was strongly associated with green tea fermentations. Tea type did not influence yeast community, with Dekkera bruxellensis, D. anomala, Zygosaccharomyces bailii and Hanseniaspora valbyensis as most dominant. This study unraveled a distinctive core microbial community which is essential for fermentation control and could lead to Kombucha quality standardization.

journal_name

FEMS Microbiol Ecol

authors

Coton M,Pawtowski A,Taminiau B,Burgaud G,Deniel F,Coulloumme-Labarthe L,Fall A,Daube G,Coton E

doi

10.1093/femsec/fix048

subject

Has Abstract

pub_date

2017-05-01 00:00:00

issue

5

eissn

0168-6496

issn

1574-6941

pii

3738478

journal_volume

93

pub_type

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