Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation.


:Qualitative and quantitative changes of microbial communities in soil microcosms during bioremediation were determined throughout one year. The soil was contaminated with 0%, 2.5%, 5%, 10% (wt/wt) of petrochemical sludge containing polynuclear aromatic hydrocarbons. We analyzed the hydrocarbon concentration in the microcosms, the number of cultivable bacteria using CFU and most probable number assays, the community structure using denaturing gradient gel electrophoresis, and the metabolic activity of soil using dehydrogenase activity and substrate-induced respiration assays. After one year of treatment, the chemical analysis suggested that the hydrocarbon elimination process was over. The biological analysis, however, showed that the contaminated microcosms suffered under long-term disturbance. The number of heterotrophic bacteria that increased after sludge addition (up to 10(8)-10(9) cells ml(-1)) has not returned to the level of the control soil (2-6 x 10(7) cells ml(-1)). The community structure in the contaminated soils differed considerably from that in the control. The substrate-induced respiration of the contaminated soils was significantly lower (approximately 10-fold) and the dehydrogenase activity was significantly higher (20-40-fold) compared to the control. Changes in the community structure of soils depended on the amount of added sludge. The species, which were predominant in the sludge community, could not be detected in the contaminated soils.


FEMS Microbiol Ecol


Del Panno MT,Morelli IS,Engelen B,Berthe-Corti L




Has Abstract


2005-07-01 00:00:00














  • Two dnaB genes are associated with the origin of replication of pQBR55, an exogenously isolated plasmid from the rhizosphere of sugar beet.

    abstract::Plasmid pQBR55 ( approximately 149 kb) represents one of five (Groups I-V) genetically distinct transfer proficient, mercury resistance plasmid groups that have been observed in the phytosphere pseudomonad community at a single geographic location in Oxford, UK. A 4.9-kb HindIII fragment was cloned from pQBR55 (a Grou...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Turner SL,Lilley AK,Bailey MJ

    更新日期:2002-11-01 00:00:00

  • Summer distribution and diversity of aerobic anoxygenic phototrophic bacteria in the Mediterranean Sea in relation to environmental variables.

    abstract::Aerobic anoxygenic phototrophic bacteria (AAP) represent an important fraction of bacterioplankton assemblages in various oceanic regimes. Although their abundance and distribution have been explored recently in diverse oceanic regions, the environmental factors controlling the population structure and diversity of th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Lehours AC,Cottrell MT,Dahan O,Kirchman DL,Jeanthon C

    更新日期:2010-11-01 00:00:00

  • Specialized microbiome of the cave-dwelling sponge Plakina kanaky (Porifera, Homoscleromorpha).

    abstract::The recent description of the polychromatic sponge Plakina kanaky revealed original microsymbionts, with some morphotypes recorded for the first time in Homoscleromorpha and others never before observed in other sponge groups. Illumina 16S amplicon sequencing was used to characterize this microbial community by compar...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Ruiz C,Villegas-Plazas M,Thomas OP,Junca H,Pérez T

    更新日期:2020-04-01 00:00:00

  • Impacts of copper and lead exposure on prokaryotic communities from contaminated contrasted coastal seawaters: the influence of previous metal exposure.

    abstract::Our understanding of environmental factors controlling prokaryotic community is largely hampered by the large environmental variability across spatial scales (e.g. trace metal contamination, nutrient enrichment and physicochemical variations) and the broad diversity of bacterial pre-exposure to environmental factors. ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Coclet C,Garnier C,Durrieu G,D'onofrio S,Layglon N,Briand JF,Misson B

    更新日期:2020-06-01 00:00:00

  • Methanogen communities along a primary succession transect of mire ecosystems.

    abstract::Peat accumulating mires are important sources of the greenhouse gas methane. Methane emissions and methanogenic Archaea communities have been shown to differ between fens and bogs, implying that mire succession includes an ecological succession in methanogen communities. We investigated methane production and the meth...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Merilä P,Galand PE,Fritze H,Tuittila ES,Kukko-Oja K,Laine J,Yrjälä K

    更新日期:2006-02-01 00:00:00

  • Revisiting life strategy concepts in environmental microbial ecology.

    abstract::Microorganisms are physiologically diverse, possessing disparate genomic features and mechanisms for adaptation (functional traits), which reflect on their associated life strategies and determine at least to some extent their prevalence and distribution in the environment. Unlike animals and plants, there is an unpre...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Ho A,Di Lonardo DP,Bodelier PL

    更新日期:2017-03-01 00:00:00

  • Selective elimination of aphid endosymbionts: effects of antibiotic dose and host genotype, and fitness consequences.

    abstract::Multiple endosymbionts commonly coexist in the same host insects. In order to gain an understanding of the biological roles of the individual symbionts in such complex systems, experimental techniques for enabling the selective removal of a specific symbiont from the host are of great importance. By using the pea aphi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Koga R,Tsuchida T,Sakurai M,Fukatsu T

    更新日期:2007-05-01 00:00:00

  • Multiple physiological states of a Pseudomonas fluorescens DR54 biocontrol inoculant monitored by a new flow cytometry protocol.

    abstract::A new fluorescence staining and flow cytometry protocol was developed to monitor several physiological states in biocontrol strain Pseudomonas fluorescens DR54 during storage survival in a stationary-phase culture, preparation of clay carrier for seed formulation, and establishment in a sugar beet spermosphere. The hi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Nielsen TH,Sjøholm OR,Sørensen J

    更新日期:2009-03-01 00:00:00

  • Diet shapes the gut microbiota of the omnivorous cockroach Blattella germanica.

    abstract::The gut microbiota of insects contributes positively to the physiology of its host mainly by participating in food digestion, protecting against pathogens, or provisioning vitamins or amino acids, but the dynamics of this complex ecosystem is not well understood so far. In this study, we have characterized the gut mic...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Pérez-Cobas AE,Maiques E,Angelova A,Carrasco P,Moya A,Latorre A

    更新日期:2015-04-01 00:00:00

  • Siderophore-mediated cooperation and virulence in Pseudomonas aeruginosa.

    abstract::Why should organisms cooperate with each other? Helping close relatives that are likely to share the same genes (kin selection) is one important explanation that is likely to apply across taxa. The production of metabolically costly extracellular iron-scavenging molecules (siderophores) by microorganisms is a cooperat...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Buckling A,Harrison F,Vos M,Brockhurst MA,Gardner A,West SA,Griffin A

    更新日期:2007-11-01 00:00:00

  • Detection of luciferase gene sequences in nonluminescent bacteria from the Chesapeake Bay.

    abstract::A 745-bp luxA fragment was amplified from Vibrio harveyi (UM 1503), radiolabeled, and used as a probe to detect and quantify luxA genotypes in culturable bacterial populations from the Chesapeake Bay. DNA samples from 53 reference strains were also examined for this gene. The luxA-positive bacteria comprised from 0-6%...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Ramaiah N,Chun J,Ravel J,Straube WL,Hill RT,Colwell RR

    更新日期:2000-07-01 00:00:00

  • Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25-3T studied in an in vitro gut model.

    abstract::Butyrate-producing bacteria are promising probiotic candidates to target microbial dysbiosis in gastrointestinal disorders like inflammatory bowel diseases. Butyricicoccus pullicaecorum 25-3(T), a butyrate-producing clostridial cluster IV strain, is such a candidate. Little is known about its abundance in the colon mi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Geirnaert A,Wang J,Tinck M,Steyaert A,Van den Abbeele P,Eeckhaut V,Vilchez-Vargas R,Falony G,Laukens D,De Vos M,Van Immerseel F,Raes J,Boon N,Van de Wiele T

    更新日期:2015-06-01 00:00:00

  • Bacterial communities associated with healthy and Acropora white syndrome-affected corals from American Samoa.

    abstract::Acropora white syndrome (AWS) is characterized by rapid tissue loss revealing the white underlying skeleton and affects corals worldwide; however, reports of causal agents are conflicting. Samples were collected from healthy and diseased corals and seawater around American Samoa and bacteria associated with AWS charac...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Wilson B,Aeby GS,Work TM,Bourne DG

    更新日期:2012-05-01 00:00:00

  • Mitochondrial sequences of Rhipicephalus and Coxiella endosymbiont reveal evidence of lineages co-cladogenesis.

    abstract::Rhipicephalus ticks are competent vectors of several pathogens, such as Spotted Fever Group Rickettsiae (SFGR) and many Babesia species. Within this genus, different R. sanguineus s.l. lineages show an unequal vector competence and resistance regarding some pathogenic strains. Current literature supports that tick end...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Coimbra-Dores MJ,Jaarsma RI,Carmo AO,Maia-Silva M,Fonville M,da Costa DFF,Brandão RML,Azevedo F,Casero M,Oliveira AC,Afonso SMS,Sprong H,Rosa F,Dias D

    更新日期:2020-06-01 00:00:00

  • Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community.

    abstract::Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Durand L,Zbinden M,Cueff-Gauchard V,Duperron S,Roussel EG,Shillito B,Cambon-Bonavita MA

    更新日期:2010-02-01 00:00:00

  • High-frequency monitoring of the genetic diversity and the potential toxicity of a Microcystis aeruginosa bloom in a French shallow lake.

    abstract::During cyanobacterial blooms, processes influencing the population dynamics of blooming species remain partially unexplained. To provide new information, we performed a high-frequency monitoring – every 2 days at six sampling points – of a Microcystis aeruginosa population blooming in a shallow lake. At each sampling ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Pobel D,Godon JJ,Humbert JF,Robin J

    更新日期:2012-01-01 00:00:00

  • Bacterial invasion potential in water is determined by nutrient availability and the indigenous community.

    abstract::In drinking water (DW) and the distribution systems, bacterial growth and biofilm formation have to be controlled both for limiting taste or odour development and preventing clogging or biocorrosion problems. After a contamination with undesired bacteria, factors like nutrient availability and temperature will influen...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Van Nevel S,De Roy K,Boon N

    更新日期:2013-09-01 00:00:00

  • Effect of temperature stress on structure and function of the methanogenic archaeal community in a rice field soil.

    abstract::The effect of a brief (24 h) temperature shift to 4 degrees C (low-temperature stress) or 50 degrees C (high-temperature stress) was studied in methanogenic slurries of Italian rice field soil incubated at either constant 30 degrees C or 15 degrees C. Low-temperature (low-T) stress showed no effect in either the 30 de...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Wu XL,Chin KJ,Conrad R

    更新日期:2002-03-01 00:00:00

  • Impact of streambed morphology on the abundance and activity of ammonia-oxidizing bacteria.

    abstract::Ammonia oxidizers catalyze the first step of nitrification. Combined microbial nitrification-denitrification activities are essential for the removal of excess nitrogen from water bodies. In sandy streambeds, bed form structures are created by water flow and lead to the creation of heterogeneous microenvironments. The...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Yanuka-Golub K,Arnon S,Nejidat A

    更新日期:2014-10-01 00:00:00

  • Influenza A virus subtype H9N2 infection disrupts the composition of intestinal microbiota of chickens.

    abstract::The impact of low pathogenic influenza viruses such as subtype H9N2, which infect the respiratory and the gastrointestinal tracts of chickens, on microbial composition are not known. Twenty-day-old specific pathogen-free chickens were assigned to two treatment groups, control (uninfected) and H9N2-infected (challenged...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Yitbarek A,Weese JS,Alkie TN,Parkinson J,Sharif S

    更新日期:2018-01-01 00:00:00

  • Integrating conjugative elements of the SXT/R391 family from fish-isolated Vibrios encode restriction-modification systems that confer resistance to bacteriophages.

    abstract::Integrating conjugative elements (ICEs) of the SXT/R391 family have been described in Vibrios, mainly Vibrio cholerae, and other bacteria as carriers of variable gene content conferring adaptive advantages upon their hosts, including antimicrobial resistance and motility regulation. However, our knowledge on their hos...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Balado M,Lemos ML,Osorio CR

    更新日期:2013-02-01 00:00:00

  • Analysis of bacterial communities in heavy metal-contaminated soils at different levels of resolution.

    abstract::The impact of heavy metal contamination on soil bacterial communities was studied in soils amended for many years with sewage sludge contaminated with heavy metals to varying extents. At the broad level of resolution, DNA reassociation analysis indicated a dramatic decrease in bacterial diversity from 16{ omitted}000 ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Sandaa R,Torsvik V V,Enger,Daae FL,Castberg T,Hahn D

    更新日期:1999-11-01 00:00:00

  • Identification of toluene degraders in a methanogenic enrichment culture.

    abstract::Methanogenic biodegradation involves the cooperative metabolism of syntrophic bacteria that catalyse the initial attack and subsequent degradation of hydrocarbons, and methanogens that convert intermediates such as hydrogen and carbon dioxide, formate, and/or acetate to methane. The identity of syntrophic microbes and...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Fowler SJ,Gutierrez-Zamora ML,Manefield M,Gieg LM

    更新日期:2014-09-01 00:00:00

  • Shifts in bacterial community structure during succession in a glacier foreland of the High Arctic.

    abstract::Primary succession after glacier retreat has been widely studied in plant communities, but bacterial succession is still poorly understood. In particular, few studies of microbial succession have been performed in the Arctic. We investigated the shifts in bacterial community structure and soil physicochemical properti...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Kim M,Jung JY,Laffly D,Kwon HY,Lee YK

    更新日期:2017-01-01 00:00:00

  • Dryland soil microbial communities display spatial biogeographic patterns associated with soil depth and soil parent material.

    abstract::Biological soil crusts (biocrusts) are common to drylands worldwide. We employed replicated, spatially nested sampling and 16S rRNA gene sequencing to describe the soil microbial communities in three soils derived from different parent material (sandstone, shale, and gypsum). For each soil type, two depths (biocrusts,...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Steven B,Gallegos-Graves LV,Belnap J,Kuske CR

    更新日期:2013-10-01 00:00:00

  • Conjugal properties of the Sinorhizobium meliloti plasmid mobilome.

    abstract::The biology and biochemistry of plasmid transfer in soil bacteria is currently under active investigation because of its central role in prokaryote adaptation and evolution. In this work, we examined the conjugal properties of the cryptic plasmids present in a collection of the N(2)-fixing legume-symbiont Sinorhizobiu...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Pistorio M,Giusti MA,Del Papa MF,Draghi WO,Lozano MJ,Tejerizo GT,Lagares A

    更新日期:2008-09-01 00:00:00

  • Improved isolation strategies allowed the phenotypic differentiation of two Nitrospira strains from widespread phylogenetic lineages.

    abstract::The second step of nitrification, the oxidation of nitrite to nitrate, is vital for the functioning of the nitrogen cycle, but our understanding of the ecological roles of the involved microorganisms is still limited. The known diversity of Nitrospira, the most widely distributed nitrite-oxidizing bacteria, has increa...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Nowka B,Off S,Daims H,Spieck E

    更新日期:2015-03-01 00:00:00

  • Synoptic determination of living/dead and active/dormant bacterial fractions in marine sediments.

    abstract::The most widely used methods for the estimation of the living/dead fractions of bacterial cells involve specific stains that are able to reveal membrane integrity. Here, we have compared two different probes (propidium iodide and ethidium homodimer-2) that have different molecular weights and steric hindrance effects....

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Manini E,Danovaro R

    更新日期:2006-03-01 00:00:00

  • Characterization of nifH gene expression, modification and rearrangement in Nodularia spumigena strain AV1.

    abstract::The annually reoccurring blooms that characterize the surface waters of the Baltic Sea are dominated by filamentous, heterocystous cyanobacteria such as Nodularia spumigena. In a previous study, we have demonstrated that N. spumigena strain AV1 differentiates heterocysts in the absence of detectable nitrogen fixation ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Vintila S,Selao T,Norén A,Bergman B,El-Shehawy R

    更新日期:2011-08-01 00:00:00

  • Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils.

    abstract::The severity of environmental conditions at Earth's frigid zones present attractive opportunities for microbial biomining due to their heightened potential as reservoirs for novel secondary metabolites. Arid soil microbiomes within the Antarctic and Arctic circles are remarkably rich in Actinobacteria and Proteobacter...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Benaud N,Zhang E,van Dorst J,Brown MV,Kalaitzis JA,Neilan BA,Ferrari BC

    更新日期:2019-04-01 00:00:00